Recombinant construct P{UAS-Cdc42.F89}

General Information
Symbol	P{UAS-Cdc42.F89}	FlyBase ID	FBtp0008218
Feature type	transgenic_transposon	Created / Updated	1999-09-22/2001-06-04

Size		Expression data
Location-dependent role		Associated insertions	1 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Eaton et al., 1995)
Uses	CV term Qualifiers & info Reference binary system (targeted) Cdc42 (Eaton et al., 1995) characterization Cdc42 (Eaton et al., 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Eaton et al., 1995)
Component Alleles
Cdc42^F89.Scer\UAS
Reference(s)	(Medioni and Noselli, 2005, Jacinto et al., 2000, Luo, 2001.5.2, Eaton et al., 1995, Eaton et al., 1996, Stramer et al., 2005, Hozumi et al., 2006)
Molecular data	Construct: A dominant negative form of Cdc42 is expressed under the control of Scer\UAS regulatory sequences. (Eaton et al., 1995) A dominant negative form of Cdc42 is expressed under the control of Scer\UAS regulatory sequences. (Eaton et al., 1996)
Phenotypic class	cell polarity defective, with Scer\GAL4^Pxn.PS, Scer\GAL4^crq.PA (Stramer et al., 2005)
Phenotype manifest in	microchaeta & wing, with Scer\GAL4^ptc-559.1 (Eaton et al., 1996, Eaton et al., 1995) dorsal mesothoracic disc \| anterior/posterior compartment boundary, with Scer\GAL4^ptc-559.1 (Eaton et al., 1995) wing, with Scer\GAL4^ptc-559.1 (Eaton et al., 1995) embryonic epidermis \| dorsal, with Scer\GAL4^en-e16E (Jacinto et al., 2000) dorsal closure embryo, with Scer\GAL4^en-e16E (Jacinto et al., 2000) embryonic hemocyte & leading edge, with Scer\GAL4^Pxn.PS, Scer\GAL4^crq.PA (Stramer et al., 2005) embryonic hemocyte, with Scer\GAL4^Pxn.PS, Scer\GAL4^crq.PA (Stramer et al., 2005)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-Cdc42.F89} (FlyBase, 1996-, Luo, 2001.5.2) P{UAS-Cdc42.S89} (FlyBase, 1996-) UAS-cdc42^S89 (Eaton et al., 1995, Eaton et al., 1996, Jacinto et al., 2000) UAS-Cdc42 F89 (Hozumi et al., 2006) UAS-Dcdc42^L89 (Baron et al., 2000)
Secondary FlyBase IDs

References ( 9 )
Research paper	Hozumi et al., 2006, Nature 440(7085): 798--802 An unconventional myosin in Drosophila reverses the default handedness in visceral organs. [FBrf0191242] Medioni and Noselli, 2005, Development 132(13): 3069--3077 Dynamics of the basement membrane in invasive epithelial clusters in Drosophila. [FBrf0187473] Stramer et al., 2005, J. Cell Biol. 168(4): 567--573 Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration. [FBrf0184064] Baron et al., 2000, Molec. gen. Genet. 264(1-2): 98--104 Multiple roles of the Dcdc42 GTPase during wing development in Drosophila melanogaster. [FBrf0129723] Jacinto et al., 2000, Curr. Biol. 10(22): 1420--1426 Dynamic actin-based epithelial adhesion and cell matching during Drosophila dorsal closure. [FBrf0131320] Eaton et al., 1996, J. Cell Biol. 135(5): 1277--1289 Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila. [FBrf0090541] Eaton et al., 1995, J. Cell Biol. 131(1): 151--164 CDC42 and Rac1 control different actin-dependent processes in the Drosophila wing disc epithelium. [FBrf0083883]
Personal communication to FlyBase	Luo, 2001.5.2, Luo constructs and insertions. Luo constructs and insertions. [FBrf0136038]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]