A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Recombinant construct P{UAS-Cdc42.N17}

General Information
Symbol P{UAS-Cdc42.N17} FlyBase ID FBtp0000750
Feature type transgenic_transposon Created / Updated 1997-03-21/1997-12-30
Size Expression data
Location-dependent role Associated insertions 1 available
Molecular map
hide Description & Uses
Species synthetic construct
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Luo et al., 1994, FlyBase, 1992-)
Uses
CV term
Qualifiers & info
Reference
binary system (targeted)
Cdc42 | modified gene
(Luo et al., 1994)
Cdc42
(Luo et al., 1994)
characterization
Cdc42 | protein
(Luo et al., 1994)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
binary
component
(Luo et al., 1994)
modified
gene
(Luo et al., 1994)
chimeric
gene
(Luo et al., 1994)
selectable
marker
(Luo et al., 1994)
hide Component Alleles
hideCdc42N17.Scer\UAS
Reference(s) (Barrett et al., 1997, Medioni and Noselli, 2005, Bashaw et al., 2001, Sepp and Auld, 2003, Raymond et al., 2001, Jhaveri and Rodrigues, 2002, Lee and Kolodziej, 2002, Hu, 2005, Hacker and Perrimon, 1998, Suzanne et al., 2001, Harden et al., 2002, Magie et al., 2002, Schneeberger and Raabe, 2003, Lawrence et al., 2002, Fritz and VanBerkum, 2002, Wood et al., 2002, Kunwar et al., 2003, Fan et al., 2003, Fuss et al., 2004, Baron et al., 2000, Jacinto et al., 2000, Luo et al., 1994, Benlali et al., 2000, Martin-Bermudo et al., 1999, Harden et al., 1999, Ricos et al., 1999, Sotillos and Campuzano, 2000, Albin and Davis, 2004, Murphy and Montell, 1996, Eaton et al., 1995, Dobens et al., 2001, Riesgo-Escovar et al., 1996, Hutterer et al., 2004, Huelsmann et al., 2006, Stramer et al., 2005, Srahna et al., 2006)
Molecular data
Construct: A dominant-negative form of Cdc42 is expressed under the control of Scer\UAS regulatory sequences.
(Luo et al., 1994)
A dominant negative form of Cdc42 is expressed under the control of Scer\UAS regulatory sequences.
(Eaton et al., 1995)
Construct: Amino acid replacement: T17N. Scer\UAS sequences drive expression of a mutated form of Cdc42.
(Schneeberger and Raabe, 2003)
Phenotypic class
wound healing defective, with Scer\GAL4en-e16E
(Wood et al., 2002)
viable, with Scer\GAL4hs.PB
(Murphy and Montell, 1996)
neuroanatomy defective, with Scer\GAL4Mz317
(Jhaveri and Rodrigues, 2002)
lethal, with Scer\GAL4238Y
(Schneeberger and Raabe, 2003)
lethal | embryonic stage, with Scer\GAL469B
(Riesgo-Escovar et al., 1996)
lethal | partially, with Scer\GAL4elav.PLu
(Fritz and VanBerkum, 2002)
lethal | pupal stage, with Scer\GAL4how-24B
(Luo et al., 1994)
viable, with Scer\GAL4458
(Murphy and Montell, 1996)
viable, with Scer\GAL4306
(Murphy and Montell, 1996)
lethal, with Scer\GAL4198Y
(Murphy and Montell, 1996)
viable, with Scer\GAL4212
(Murphy and Montell, 1996)
cell polarity defective, with Scer\GAL4Pxn.PS, Scer\GAL4crq.PA
(Stramer et al., 2005)
Phenotype manifest in
embryonic epidermis | dorsal, with Scer\GAL4en-e16E
(Jacinto et al., 2000)
dorsal closure embryo, with Scer\GAL4en-e16E
(Jacinto et al., 2000)
embryonic/first instar larval cuticle, with Scer\GAL4ptc-559.1
(Harden et al., 1999)
embryonic/first instar larval cuticle | dorsal, with Scer\GAL4ptc-559.1
(Raymond et al., 2001)
embryonic/first instar larval cuticle | dorsal, with Scer\GAL4hs.PB
(Harden et al., 1999)
embryonic epidermis, with Scer\GAL4hs.PB
(Ricos et al., 1999)
wing hair | ectopic, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
wing vein | ectopic, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
wing margin, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
wing margin bristle, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
medial triple row, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
crossvein | cell non-autonomous | ectopic, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
wing, with Scer\GAL4Bx-MS1096
(Baron et al., 2000)
proventriculus inner layer primordium, with Scer\GAL4retn-GAL4
(Fuss et al., 2004)
proventriculus intermediate layer primordium, with Scer\GAL4retn-GAL4
(Fuss et al., 2004)
embryonic proventriculus, with Scer\GAL4retn-GAL4
(Fuss et al., 2004)
follicle cell, with Scer\GAL4Act5C.PP
(Dobens et al., 2001)
embryonic tracheal system, with Scer\GAL4btl.PS
(Lee and Kolodziej, 2002)
antenna & glial cell, with Scer\GAL4Mz317
(Jhaveri and Rodrigues, 2002)
antenna & sensory neuron, with Scer\GAL4Mz317
(Jhaveri and Rodrigues, 2002)
embryonic/first instar larval cuticle, with Scer\GAL4twi.PG
(Hacker and Perrimon, 1998)
larval midgut & embryo, with Scer\GAL448Y
(Martin-Bermudo et al., 1999)
embryonic/first instar larval cuticle | dorsal, with Scer\GAL469B
(Riesgo-Escovar et al., 1996)
dorsal appendage, with Scer\GAL4CY2
(Suzanne et al., 2001)
embryonic hemocyte & leading edge, with Scer\GAL4Pxn.PS, Scer\GAL4crq.PA
(Stramer et al., 2005)
embryonic hemocyte, with Scer\GAL4Pxn.PS, Scer\GAL4crq.PA
(Stramer et al., 2005)
Other information
hidew+mC
Reference(s) (Luo et al., 1994)
Molecular data
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
 
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
(Pirrotta, 1988)
Minigene of the white cDNA.
(Chung et al., 1993)
Deletion of w+mC 5' flanking sequences to position -305.
(Qian and Pirrotta, 1995)
Phenotypic class
courtship defective | male
(Lloyd et al., 2002, Hing and Carlson, 1996)
visible | dominant
(True et al., 1996)
visible | recessive
(Roseman et al., 1993)
eye color defective
(Soldatov et al., 1999, Hagstrom et al., 1996)
eye color defective, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Phenotype manifest in
pigment cell
(Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995)
pigment cell, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Other information
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
(FlyBase, 1992-)
descendant(s)
hide Comments
construct_comment: One of a series of UAS constructs with single amino acid modifications in the Cdc42 coding region.
(Luo et al., 1994)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
Cdc42N17
(Hutterer et al., 2004)
dnDcdc42
(Fritz and VanBerkum, 2002)
P{@w+mC@ @Cdc42N17.UAS@=@UAS-Dcdc42.N17@}
(FlyBase, 1996-)
P{@w+mC@ @Cdc42-likeN17.UAS@=@UAS-Cdc42-like.N17@}
(FlyBase, 1996-)
P{@w+mC@ @Cdc42-likeN17.UAS@=@UAS-Dcdc42.N17@}
(FlyBase, 1996-)
P{UAS-Cdc42.N17}
(FlyBase, 1996-)
P{UAS-Cdc42-like.N17}
(FlyBase, 1996-)
P{UAS-Dcdc42.N17}
(FlyBase, 1996-)
pUAST-Cdc42N17
(Lee and Kolodziej, 2002)
UAS::Cdc42N17
(Srahna et al., 2006)
UAS:Cdc42T17N
(Schneeberger and Raabe, 2003)
UAS.CDC42N17
(Lawrence et al., 2002)
UAS-Cdc42N17
(Albin and Davis, 2004, Fuss et al., 2004, Kunwar et al., 2003, Wood et al., 2002, Magie et al., 2002, Martin-Bermudo et al., 1999)
UAS-cdc42N17
(Jacinto et al., 2000, Huelsmann et al., 2006)
UAS-cdc42S89
(Jacinto et al., 2000)
UASCdc42N17
(Bashaw et al., 2001)
UAS-Dcdc42.N17
(Luo et al., 1994)
UAS-Dcdc42N17
(Sotillos and Campuzano, 2000, Baron et al., 2000, Benlali et al., 2000)
UAS-Dcdc42N17
(Sepp and Auld, 2003, Suzanne et al., 2001)
UASDcdc42N17
(Raymond et al., 2001)
UAS-DCdc42N17
(Dobens et al., 2001)
UAS-Dcdc421N17
(Harden et al., 1999)
UAS-DNCdc42
(Jhaveri and Rodrigues, 2002)
UCdc42N17
(Fan et al., 2003)
Secondary FlyBase IDs
    hide References ( 49 )
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    hide Recent research papers ( 2 )
    Huelsmann et al., 2006, Development 133(15): 2915--2924
    The PDZ-GEF dizzy regulates cell shape of migrating macrophages via Rap1 and integrins in the Drosophila embryo. [FBrf0192108]
    Srahna et al., 2006, PLoS Biol. 4(11):
    A signaling network for patterning of neuronal connectivity in the Drosophila brain. [FBrf0192599]
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