Recombinant construct P{UAS(-FRT)wg.ts}

General Information
Symbol	P{UAS(-FRT)wg.ts}	FlyBase ID	FBtp0000370
Feature type	transgenic_transposon	Created / Updated	1997-03-21/2001-12-17

Size		Expression data
Location-dependent role		Associated insertions	2 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon
Uses	CV term Qualifiers & info Reference characterization wg (Wilder and Perrimon, 1995) binary system (targeted) wg (Wilder and Perrimon, 1995)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Wilder and Perrimon, 1995) modified gene (Wilder and Perrimon, 1995) chimeric gene (Wilder and Perrimon, 1995) selectable marker (Wilder and Perrimon, 1995)
Component Alleles
wg^{l-12.Scer\UAS}
Reference(s)	(Brennan et al., 1999, Sato et al., 1999, Buratovich and Wilder, 2001, Lin et al., 2004, Weihe et al., 2004, Wilder and Perrimon, 1995, Takashima and Murakami, 2001, Baeg et al., 2001, Jhaveri et al., 2000, Buratovich et al., 2000, Yang et al., 1999, Sanson et al., 1999, Kennerdell and Carthew, 1998, Klein et al., 1997, Sanchez et al., 1997, Hacker et al., 1997, Rodriguez, 2004, Carmena et al., 1998, Alcedo et al., 1996, Baylies et al., 1995, Yoffe et al., 1995, Manoukian et al., 1995, Treisman and Rubin, 1995, Couso et al., 1995, Binari et al., 1997, van den Heuvel and Ingham, 1996, Jonsson and Knust, 1996, Johnston and Schubiger, 1996, Brook and Cohen, 1996, Lawrence et al., 1996, White et al., 1998, Liu et al., 2006, Bossing and Brand, 2006, Seto and Bellen, 2006)
Molecular data
Phenotypic class	lethal \| conditional - cold sensitive, with Scer\GAL4^dpp.blk1 (Wilder and Perrimon, 1995) visible, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) lethal \| conditional - cold sensitive, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) visible \| conditional - cold sensitive, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) visible, with Scer\GAL4^Bx-MS1096 (White et al., 1998) cell death increase \| pupal stage \| conditional - heat sensitive, with Scer\GAL4^GMR.PF (Lin et al., 2004)
Phenotype manifest in	embryonic epidermis, with Scer\GAL4^arm.PS (Sanson et al., 1999) denticle belt, with Scer\GAL4^arm.PS (Sanson et al., 1999, Kennerdell and Carthew, 1998) ommatidium, with Scer\GAL4^dpp.blk1 (Yang et al., 1999) leg, with Scer\GAL4^dpp.blk1 (Wilder and Perrimon, 1995) prothoracic unguis, with Scer\GAL4^dpp.blk1 (Wilder and Perrimon, 1995) morphogenetic furrow, with Scer\GAL4^dpp.blk1 (Treisman and Rubin, 1995) maxillary palpus \| conditional - cold sensitive, with Scer\GAL4^dpp.blk1 (Johnston and Schubiger, 1996) eye \| conditional - cold sensitive, with Scer\GAL4^dpp.blk1 (Johnston and Schubiger, 1996) antenna \| conditional - cold sensitive, with Scer\GAL4^dpp.blk1 (Johnston and Schubiger, 1996) leg \| conditional - cold sensitive, with Scer\GAL4^dpp.blk1 (Johnston and Schubiger, 1996) wing, with Scer\GAL4^sd-SG29.1 (Klein et al., 1997, Brennan et al., 1999) wing vein, with Scer\GAL4^sd-SG29.1 (Klein et al., 1997, Brennan et al., 1999) wing & macrochaeta, with Scer\GAL4^sd-SG29.1 (Klein et al., 1997) trichome of the posterior wing margin, with Scer\GAL4^sd-SG29.1 (Klein et al., 1997) wing margin bristle \| ectopic, with Scer\GAL4^sd-SG29.1 (Brennan et al., 1999) embryonic epidermis, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) leg, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) prothoracic tarsal segment 1 transverse row, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) prothoracic unguis, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) mesothoracic leg, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) ventral thoracic disc, with Scer\GAL4^h-1J3 (Wilder and Perrimon, 1995) epidermis, with Scer\GAL4^h-1J3 (Yoffe et al., 1995) denticle belt, with Scer\GAL4^h-1J3 (Yoffe et al., 1995) somatic muscle, with Scer\GAL4^69B (Baylies et al., 1995) wing, with Scer\GAL4^Bx-MS1096 (White et al., 1998) wing vein \| ectopic, with Scer\GAL4^Bx-MS1096 (White et al., 1998) wing margin bristle \| ectopic, with Scer\GAL4^Bx-MS1096 (White et al., 1998) sensillum coeloconicum \| supernumerary, with Scer\GAL4^Dll-981 (Jhaveri et al., 2000) epidermis, with Scer\GAL4^prd.RG1 (Yoffe et al., 1995) denticle belt, with Scer\GAL4^prd.RG1 (Yoffe et al., 1995) eye photoreceptor cell & pupa \| conditional ts, with Scer\GAL4^GMR.PF (Lin et al., 2004) embryonic hindgut, with Scer\GAL4^byn-Gal4 (Takashima and Murakami, 2001) embryonic large intestine, with Scer\GAL4^byn-Gal4 (Takashima and Murakami, 2001) embryonic rectum, with Scer\GAL4^byn-Gal4 (Takashima and Murakami, 2001) embryonic epidermis \| dorsal, with Scer\GAL4^da.G32 (Buratovich et al., 2000) dorsal denticle, with Scer\GAL4^da.G32 (Buratovich et al., 2000) maxillary palpus \| conditional - cold sensitive, with Scer\GAL4^ptc-559.1 (Johnston and Schubiger, 1996) antenna \| conditional - cold sensitive, with Scer\GAL4^ptc-559.1 (Johnston and Schubiger, 1996) labial segment \| conditional - cold sensitive, with Scer\GAL4^ptc-559.1 (Johnston and Schubiger, 1996) head \| conditional - cold sensitive, with Scer\GAL4^ptc-559.1 (Johnston and Schubiger, 1996) leg \| conditional - cold sensitive, with Scer\GAL4^ptc-559.1 (Johnston and Schubiger, 1996) genitalia \| conditional - cold sensitive, with Scer\GAL4^ptc-559.1 (Johnston and Schubiger, 1996)
Other information
w^+mC
Reference(s)	(Wilder and Perrimon, 1995)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{UAS(FRT.polyA)wg.ts} (Wilder and Perrimon, 1995)
descendant(s)
Comments
	report comment: Produces a temperature-sensitive wg protein. (Wilder and Perrimon, 1995) construct_comment: Generated in vivo by excision of FRT cassette from P{UAS-FRT-wg.ts}. (Wilder and Perrimon, 1995) construct_comment: Carries single FRT site. (Wilder and Perrimon, 1995)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{@w^+mC@ @wg^l-12.UAS@ @FRT@=@UAS-wg.ts@} (FlyBase, 1996-) P{UAS(-FRT)wg.ts} (FlyBase, 1996-) P{UAS-wg.ts} (FlyBase, 1996-) P{w^+mC wg^l-12.UAS=UAS-wg.ts} (FlyBase, 1996-) UAS-wg (Weihe et al., 2004, Baeg et al., 2001, Yang et al., 1999, Baylies et al., 1995, Liu et al., 2006, Seto and Bellen, 2006) UAS-Wg (Carmena et al., 1998) UASwg (Manoukian et al., 1995) UASwg^IL (Couso et al., 1995) UAS-wg^ts (Rodriguez, 2004, Takashima and Murakami, 2001, Jhaveri et al., 2000, Sato et al., 1999, Sanson et al., 1999, Treisman and Rubin, 1995, Bossing and Brand, 2006) UAS-Wg^ts (Lin et al., 2004, Kennerdell and Carthew, 1998) UASwg^ts (Buratovich and Wilder, 2001, Buratovich et al., 2000, Wilder and Perrimon, 1995) UASwgIL (Brennan et al., 1999)
Secondary FlyBase IDs

References ( 47 )

Generate a list of	All references relating to this transgenic transposon ( 47 )

List References by type	Research paper ( 45 ) Review ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 3 )
	Bossing and Brand, 2006, Development 133(6): 1001--1012 Determination of cell fate along the anteroposterior axis of the Drosophila ventral midline. [FBrf0190277] Liu et al., 2006, Dev. Biol. 290(2): 373--385 Hedgehog and RAS pathways cooperate in the anterior-posterior specification and positioning of cardiac progenitor cells. [FBrf0190138] Seto and Bellen, 2006, J. Cell Biol. 173(1): 95--106 Internalization is required for proper Wingless signaling in Drosophila melanogaster. [FBrf0190944] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2006 Show reviews ...