A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Dmel\P{lacW}v(2)k05816k05816 Insertion

General Information
Symbol Dmel\P{lacW}v(2)k05816k05816 Species D. melanogaster
Name FlyBase ID FBti0006767
Feature type transposable_element_insertion_site Created / Updated 1998-01-03/2000-11-20
Description
Inserted element P{lacW} Localized function
Affected gene(s) v(2)k05816 Expression data
Causes allele(s) v(2)k05816k05816 Viability / fertility
LINE ID l(2)k05816 Stock availability
Genomic Location
Chromosomal location 2L ( 23C5 ) Sequence location 2L:3,046,753..3,046,753 [+]
Map ( GBrowse ) detailed view FBti0025819 FBti0018662 FBti0066208 FBti0040881 FBti0064820 FBti0055433 FBti0033482 FBti0006767 FBti0027105 FBti0007668 FBti0050808 FBti0067513 FBti0046123 FBti0046230 FBti0029668 FBti0024467 FBti0029843 FBti0007638 FBti0029794 FBti0066296 FBti0045815 FBti0066067 FBti0066295 FBti0100819 FBti0033325
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:3,046,753..3,046,753 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
23C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
23D1--23D2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
23D1-23D2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Chromosome in situ
hide Sequence Data
Flanking sequence
AQ025771
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role 		 
Size 10.691Kb
Associated alleles
w+mC
Ecol\lacZP\T.W
Molecular map
Transposon class
Element type
hide Affected Gene(s)
Insertion within gene
Insertion may
affect gene
v(2)k05816
(BDGP Project Members, 1994-1999)
Orientation
(relative to gene)
Gene
Orientation
References
 
 
 
hide Alleles and Phenotypes
Causes alleles
v(2)k05816k05816
(BDGP Project Members, 1994-1999, BDGP Project Members, 2000-, Levis, 2001.11.16)
Lethality
References
Sterility
References
hide Phenotype Manifest In
wing
(BDGP Project Members, 2000-)
leg
(BDGP Project Members, 2000-)
hide Detailed Description
Statement
Reference
v(2)k05816k05816/Df(2L)JS17 hemizygotes have collapsed shreds of wings which look rolled up along the proximal distal axis. Legs have abnormalities distal to the basitarsus, are spindly and malformed. These phenotypes are 100% penetrant.
(BDGP Project Members, 2000-)
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
Additional Information
Statement
Reference
Assay mode
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k05816
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Kyoto
102421
Df(1)w67c23, y1 ; P{lacW}v(2)k05816k05816 / CyO
Bloomington
10580
y1 w67c23; P{lacW}v(2)k05816k05816, l(2)k05816k05816/CyO
hide Linkouts
hide Comments
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
Location 2L:3046752-3046753 determined by FlyBase alignment of dbGSS accession AQ025771 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
Separable from l(2)k05816k05816.
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)k05816k05816
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}v(2)k05816k05816
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
    hide References ( 8 )
    Research paper
    Bellen et al., 2004, Genetics 167(2): 761--781
    The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
    Spradling et al., 1999, Genetics 153(1): 135--177
    The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
    Personal communication to FlyBase
    Levis, 2001.11.16, v(2)k05816 has a P insertion in CG3524.
    v(2)k05816 has a P insertion in CG3524. [FBrf0141845]
    FlyBase analysis
    FlyBase, 2005, Assessment of transgenic construct insertion sites.
    Assessment of transgenic construct insertion sites. [FBrf0184339]
    FlyBase, 1992-, FlyBase curation
    FlyBase curation. [FBrf0105495]
    Computer file
    Gene Disruption Project members, 2001-, [title not yet available]
    [title not yet available] [FBrf0132177]
    BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
    Berkeley Drosophila Genome Project. [FBrf0125078]
    BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
    Berkeley Drosophila Genome Project. [FBrf0067338]