HomeReference Report
| Reference | |
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| Citation | Brennecke, J. (2005.7.3). mir-2b and miR-6 constructs. |
| FlyBase ID | FBrf0188415 |
| Type of publication | Personal communication to FlyBase |
| Offprint Available | No |
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| Text of personal communication |
From: Julius Brennecke <brenneck@XXXXXXXXXXXXXXX>
To: cy200@XXXXXXXXXXXXXXX Subject: Re: Fwd: FlyBase Query (cy2593) Date: Sun, 3 Jul 2005 16:15:05 \+0200 (15:15 BST) Dear Chihiro, here is the requested info. the mir-2b construct is based on miR-2b-2 (the miRNA in the spitz intron). the miR-6 construct was obtained from Eric Lai as indicated in the paper and it contains all miR-6 hairpins as they are clustered in the genome. i'll be happy to answer any further questions. best, julius >> Dear Dr Cohen, >> >> I am currently curating your paper for FlyBase: >> >> Brennecke et al., 2004, PLoS Biol. 3(3): 404--418 >> >> I have a couple of quick questions I was hoping you could answer for me: >> >> mir-2b >> ====== >> You make a UAS construct using mir-2b sequences. However we have two >> genes in our records mir-2b-1, mir-2b-2. Could you tell me which one of >> these you used to make your construct? >> >> mir-2b-1 >> CR32990 >> 29A1 >> AE003620 >> AJ421759 >> MIR:MI0000119 >> >> mir-2b-2 >> CR32964 >> 37F2 >> AE003663 >> AJ421760 >> MIR:MI0000120 >> >> mir-6 >> ====== >> Likewise with mir-6. You make a UAS construct using mir-2b sequences. >> However we have three genes in our records that this could be. Could >> you tell me which one of these you used to make your construct? >> >> mir-6-1 >> CR33004 >> 56E1 >> AE003795 >> AJ421764 >> MIR:MI0000124 >> Rfam:RF00143 >> >> mir-6-2 >> CR33040 >> 56E1 >> AE003795 >> AJ421765 >> MIR:MI0000125 >> Rfam:RF00143 >> >> mir-6-3 >> CR33039 >> 56E1 >> AE003795 >> AJ421766 >> MIR:MI0000126 >> Rfam:RF00143 >> >> Best wishes, >> >> Chihiro |
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Related Publications
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| Research paper | Principles of MicroRNA-Target Recognition. Brennecke, 2005, PLoS Biol. 3(3): 404--418 [FBrf0188411] |
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| Language of publication | English |
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| Language of publication | English |
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| Alleles (4)
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| Constructs (2)
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