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Reference
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| Citation |
Kiehart, D.P., Franke, J.D., Chee, M.K., Montague, R.A., Chen, T.L., Roote, J., Ashburner, M. (2004). Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA. Genetics 168(3): 1337--1352. |
| FlyBase ID |
FBrf0180276 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
15579689 |
| PubMed Abstract |
Myosin VIIs provide motor function for a wide range of eukaryotic processes. We demonstrate that mutations in crinkled (ck)
disrupt the Drosophila myosin VIIA heavy chain. The ck/myoVIIA protein is present at a low level throughout fly development
and at the same level in heads, thoraxes, and abdomens. Severe ck alleles, likely to be molecular nulls, die as embryos or
larvae, but all allelic combinations tested thus far yield a small fraction of adult "escapers" that are weak and infertile.
Scanning electron microscopy shows that escapers have defects in bristles and hairs, indicating that this motor protein plays
a role in the structure of the actin cytoskeleton. We generate a homology model for the structure of the ck/myosin VIIA head
that indicates myosin VIIAs, like myosin IIs, have a spectrin-like, SH3 subdomain fronting their N terminus. In addition,
we establish that the two myosin VIIA FERM repeats share high sequence similarity with only the first two subdomains of the
three-lobed structure that is typical of canonical FERM domains. Nevertheless, the approximately 100 and approximately 75
amino acids that follow the first two lobes of the first and second FERM domains are highly conserved among myosin VIIs, suggesting
that they compose a conserved myosin tail homology 7 (MyTH7) domain that may be an integral part of the FERM domain or may
function independently of it. Together, our data suggest a key role for ck/myoVIIA in the formation of cellular projections
and other actin-based functions required for viability.
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| Biosis |
2005.125519 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
| Page range |
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| Publisher |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
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Data from Reference
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| Aberrations (2)
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| Alleles (9)
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| Genes (4)
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| Insertions (3)
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