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Citation	Cook, H.A., Koppetsch, B.S., Wu, J., Theurkauf, W.E. (2004). The Drosophila SDE3 homolog armitage is required for oskar mRNA silencing and embryonic axis specification. Cell 116(6): 817--829.
FlyBase ID	FBrf0174441
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	15035984
PubMed Abstract	Polarization of the microtubule cytoskeleton during early oogenesis is required to specify the posterior of the Drosophila oocyte, which is essential for asymmetric mRNA localization during mid-oogenesis and for embryonic axis specification. The posterior determinant oskar mRNA is translationally silent until mid-oogenesis. We show that mutations in armitage and three components of the RNAi pathway disrupt oskar mRNA translational silencing, polarization of the microtubule cytoskeleton, and posterior localization of oskar mRNA. armitage encodes a homolog of SDE3, a presumptive RNA helicase involved in posttranscriptional gene silencing (RNAi) in Arabidopsis, and is required for RNAi in Drosophila ovaries. Armitage forms an asymmetric network associated with the polarized microtubule cytoskeleton and is concentrated with translationally silent oskar mRNA in the oocyte. We conclude that RNA silencing is essential for establishment of the cytoskeletal polarity that initiates embryonic axis specification and for translational control of oskar mRNA.
Biosis	2004.261277
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Language of publication	English
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Abbreviation	Cell
Title	Cell
Authors
Volume range	1-
Year range	1974-
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Publisher	Cell Press
Place of publication	Cambridge, MA
Language of publication	English
ISBN/ISSN	0092-8674
CODEN	CELLB5
Data from Reference
Aberrations (2)
	Df(3L)E1 Df(3L)HD1
Alleles (11)
	Scer\GAL4^nos.PG T:Avic\GFP^EGFP armi¹ armi^72.1 armi^{Scer\UAS.P\T.T:Avic\GFP-EGFP} armi^rev39.2 aub^HN aub^QC42 mael^r20 osk¹ spn-E¹
Constructs (2)
	P{GAL4-nos.NGT} P{UASp-armi-EGFP}
Genes (15)
	CycJ Scer\GAL4 T:Avic\GFP armi aub bcd grk hts mael osk spn-E vas αTub67C αTub84B αTub84D
Insertions (1)
	P{lacW}armi¹