Reference Report

Reference
Citation	Zhang, Y.Q., Bailey, A.M., Matthies, H.J.G., Renden, R.B., Smith, M.A., Speese, S.D., Rubin, G.M., Broadie, K. (2001). Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. Cell 107(5): 591--603.
FlyBase ID	FBrf0141416
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	11733059
PubMed Abstract	Fragile X mental retardation gene (FMR1) encodes an RNA binding protein that acts as a negative translational regulator. We have developed a Drosophila fragile X syndrome model using loss-of-function mutants and overexpression of the FMR1 homolog (dfxr). dfxr nulls display enlarged synaptic terminals, whereas neuronal overexpression results in fewer and larger synaptic boutons. Synaptic structural defects are accompanied by altered neurotransmission, with synapse type-specific regulation in central and peripheral synapses. These phenotypes mimic those observed in mutants of microtubule-associated Futsch. Immunoprecipitation of dFXR shows association with futsch mRNA, and Western analyses demonstrate that dFXR inversely regulates Futsch expression. dfxr futsch double mutants restore normal synaptic structure and function. We propose that dFXR acts as a translational repressor of Futsch to regulate microtubule-dependent synaptic growth and function.
Biosis	2002.42664
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Language of publication	English
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Abbreviation	Cell
Title	Cell
Authors
Volume range	1-
Year range	1974-
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Publisher	Cell Press
Place of publication	Cambridge, MA
Language of publication	English
ISBN/ISSN	0092-8674
CODEN	CELLB5
Data from Reference
Aberrations (1)
	Df(3R)by62
Alleles (15)
	Fmr1^9N Fmr1^EP3422 Fmr1^EP3517 Fmr1^{I307N.Z.Scer\UAS} Fmr1^Scer\UAS.cZa Fmr1^Δ113M Fmr1^Δ192N Fmr1^Δ50M Fmr1^Δ83M Scer\GAL4^G7 Scer\GAL4^Mhc.PW Scer\GAL4^elav-C155 Scer\GAL4^hs.2sev futsch^EP1419 futsch^N94
Constructs (3)
	P{GAL4-Mhc.W} P{UAS-Fmr1.I307N.Z} P{UAS-Fmr1.Z}
Genes (6)
	Csp Fmr1 Scer\GAL4 dlg1 futsch syt
Insertions (5)
	P{EP}Fmr1^EP3422 P{EP}Fmr1^EP3517 P{EP}futsch^EP1419 P{GAL4}G7 P{GawB}elav^C155