Reference Report

Reference
Citation	Harris, A.N., Macdonald, P.M. (2001). aubergine encodes a Drosophila polar granule component required for pole cell formation and related to eIF2C. Development 128(14): 2823--2832.
FlyBase ID	FBrf0138362
Type of publication	Research paper
Offprint Available	No
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PubMed ID	11526087
PubMed Abstract	In Drosophila oocytes, activation of Oskar translation from a transcript localized to the posterior pole is an essential step in the organization of the pole plasm, specialized cytoplasm that contains germline and abdominal body patterning determinants. Oskar is a component of polar granules, large particles associated with the pole plasm and the germline precursor pole cells of the embryo. aubergine mutants fail to translate oskar mRNA efficiently and are thus defective in posterior body patterning and pole cell formation. We have found that Aubergine protein is related to eukaryotic translation initiation factor 2C and suggest how it may activate translation. In addition, we found that Aubergine was recruited to the posterior pole in a vas-dependent manner and is itself a polar granule component. Consistent with its presence in these structures, Aubergine is required for pole cell formation independently of its initial role in oskar translation. Unlike two other known polar granule components, Vasa and Oskar, Aubergine remains cytoplasmic after pole cell formation, suggesting that the roles of these proteins diverge during embryogenesis.
Biosis	2001.398359
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Language of publication	English
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Abbreviation	Development
Title	Development
Authors
Volume range	99-
Year range	1987-
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Place of publication	Cambridge
Language of publication	English
ISBN/ISSN	0950-1991
CODEN	DEVPED
Data from Reference
Alleles (16)
	Scer\GAL4^{nos.UTR.T:Hsim\VP16} T:Avic\GFP^m6 aub^HN aub^K86 aub^N11 aub^{Scer\UAS.P\T.T:Avic\GFP-m6} aub^tMR-1 aub^tMR-3 aub^unspecified osk^short.bcd stau² vas¹ vas⁴ vas^AS vas^{αTub67C.T:Avic\GFP} w^+mC
Constructs (6)
	P{GAL4::VP16-nos.UTR} P{GFP-Vas} P{UASp-GFP-aub.H} P{aub^tMR-1} P{aub^tMR-3} P{short.osk-bcd}
Genes (12)
	AGO1 AGO2 RpL9 Scer\GAL4 T:Avic\GFP T:Hsim\VP16 aub osk piwi stau vas w
Insertions (1)
	P{NM}31E