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Reference
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| Citation |
Tillib, S., Petruk, S., Sedkov, Y., Kuzin, A., Fujioka, M., Goto, T., Mazo, A. (1999). Trithorax- and polycomb-group response elements within an Ultrabithorax transcription maintenance unit consist of closely
situated but separable sequences. Molec. Cell. Biol. 19(7): 5189--5202. |
| FlyBase ID |
FBrf0109119 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
10373568 |
| PubMed Abstract |
In Drosophila, two classes of genes, the trithorax group and the Polycomb group, are required in concert to maintain gene
expression by regulating chromatin structure. We have identified Trithorax protein (TRX) binding elements within the bithorax
complex and have found that within the bxd/pbx regulatory region these elements are functionally relevant for normal expression
patterns in embryos and confer TRX binding in vivo. TRX was localized to three closely situated sites within a 3-kb chromatin
maintenance unit with a modular structure. Results of an in vivo analysis showed that these DNA fragments (each approximately
400 bp) contain both TRX- and Polycomb-group response elements (TREs and PREs) and that in the context of the endogenous Ultrabithorax
gene, all of these elements are essential for proper maintenance of expression in embryos. Dissection of one of these maintenance
modules showed that TRX- and Polycomb-group responsiveness is conferred by neighboring but separable DNA sequences, suggesting
that independent protein complexes are formed at their respective response elements. Furthermore, we have found that the activity
of this TRE requires a sequence (approximately 90 bp) which maps to within several tens of base pairs from the closest neighboring
PRE and that the PRE activity in one of the elements may require a binding site for PHO, the protein product of the Polycomb-group
gene pleiohomeotic. Our results show that long-range maintenance of Ultrabithorax expression requires a complex element composed
of cooperating modules, each capable of interacting with both positive and negative chromatin regulators.
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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Published In
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| Abbreviation |
Molec. Cell. Biol. |
| Title |
Molecular and Cellular Biology |
| Authors |
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| Volume range |
1- |
| Year range |
1981- |
| Page range |
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| Publisher |
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| Place of publication |
Washington, DC |
| Language of publication |
English |
| ISBN/ISSN |
0270-7306 |
| CODEN |
MCEBD4 |
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Data from Reference
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| Alleles (37)
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| Constructs (21)
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| Genes (11)
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