Reference Report

Reference
Citation	Tinker, R., Silver, D., Montell, D.J. (1998). Requirement for the vasa RNA helicase in gurken mRNA localization. Dev. Biol. 199(1): 1--10.
FlyBase ID	FBrf0103384
Type of publication	Research paper
Offprint Available	No
External Crossreferences
PubMed ID	9676188
PubMed Abstract	Localization of specific mRNAs to distinct sites within the Drosophila oocyte is an early and key step in establishing the anterior-posterior and dorsal-ventral axes. We describe a new function for the RNA helicase encoded by the "posterior" group gene vasa (vas) in control of localization of the mRNA encoded by the "dorsal-ventral" patterning gene gurken (grk). Two new ethyl methane sulfonate-induced, female sterile alleles of vas have been isolated. In these mutants grk mRNA fails to become localized properly and GRK protein is barely detectable. Surprisingly fs(1)K10, a recessive female sterile mutation that results in mislocalization of GRK mRNA to the anterior end of the oocyte, is epistatic to these vas alleles. This result demonstrates that GRK protein levels sufficient to dorsalize the egg chamber can accumulate in vas mutants, if fs(1)K10 is also mutant. Taken together these results suggest that regulation of GRK mRNA localization normally occurs, directly or indirectly, through the VAS RNA-dependent RNA helicase and may suggest that accumulation of GRK protein normally depends on GRK mRNA localization.
Biosis	1391213
Zoological record
Associated Information
Comments
Text of personal communication
Associated files
Related Publications

Also Published As

Other Reference Information
Secondary IDs
Language of publication	English
Additional language(s) of abstract
ISBN
Place of publication
Published In
Abbreviation	Dev. Biol.
Title	Developmental Biology
Authors
Volume range	1-
Year range	1959-
Page range
Publisher
Place of publication	New York
Language of publication	English
ISBN/ISSN	0012-1606
CODEN	DEBIAO
Data from Reference
Aberrations (9)
	Df(2L)64j Df(2L)A267 Df(2L)A48 Df(2L)TE35D-18 Df(2L)b84a2 Df(2L)b84a7 Df(2L)b87e25 Df(2L)b88c75 In(2L)b82a1
Alleles (8)
	Ecol\lacZ⁶³⁵⁶ Scer\GAL4^CY2 T:λ\cI^DD btl::Egfr^{Scer\UAS.T:λ\cI-DD} fs(1)K10^unspecified vas^6356-001 vas^6356-005 vas^RJ36
Constructs (1)
	P{UAS-Egfr.λtop}
Genes (14)
	Ecol\lacZ Egfr Scer\GAL4 Su(H) T:λ\cI TfIIS bcd btl btl::Egfr ck fs(1)K10 grk vas vig
Insertions (2)
	P{GawB}CY2 P{PZ}6356