| Citation |
Dooijes, D., van Beest, M., van de Wetering, M., Boulanger, G., Jones, T., Clevers, H., Mortin, M.A. (1998). Genomic organization of the segment polarity gene pan in Drosophila melanogaster. Molec. gen. Genet. 258(1-2): 45--52.
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| PubMed Abstract |
We previously described the molecular cloning of a mammalian T cell factor 1 (TCF-1)-like protein from Drosophila melanogaster,
encoded by the pangolin (pan) locus, and demonstrated that it consists of a DNA binding domain similar to that of other high
mobility group proteins and a protein-protein interaction domain that binds beta-catenin (Armadillo in Drosophila) but that
it lacks a transcriptional activation domain. Here we show that the pan locus spans approximately 50 kb and the mRNA results
from the splicing of 13 exons. We note remarkable conservation of the exon/intron boundaries between the human and D. melanogaster
genes, suggesting that they share a common ancestor. Chromosomal in situ hybridization locates pan to the base of chromosome
4, near the cubitus interruptus locus. Restriction map and sequence analyses confirm their close proximity. The small fourth
chromosome undergoes little or no recombination and was previously reported to lack DNA polymorphisms; however, we note two
DNA polymorphisms occurring in three combinations within the pan locus, demonstrating the presence of synonymous substitutions
and the past occurrence of recombination. We present evidence suggesting that the protein encoded by pan is more similar to
mammalian TCF-1 and Caenorhabditis elegans POP-1 than to mammalian LEF-1.
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