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Reference
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| Citation |
Fehon, R.G., Oren, T., Lajeunesse, D.R., Melby, T.E., McCartney, B.M. (1997). Isolation of mutations in the Drosophila homologues of the human Neurofibromatosis 2 and yeast CDC42 genes using a simple
and efficient reverse-genetic method. Genetics 146(1): 245--252. |
| FlyBase ID |
FBrf0093443 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
9136014 |
| PubMed Abstract |
Reverse genetic analysis in Drosophila has been greatly aided by a growing collection of lethal P transposable element insertions
that provide molecular tags for the identification of essential genetic loci. However, because the screens performed to date
primarily have generated autosomal P-element insertions, this collection has not been as useful for performing reverse genetic
analysis of X-linked genes. We have designed a reverse genetic screen that takes advantage of the hemizygosity of the X chromosome
in males together with a cosmid-based transgene that serves as an autosomally linked duplication of a small region of the
X chromosome. The efficacy and efficiency of this method is demonstrated by the isolation of mutations in Drosophila homologues
of two well-studied genes, the human Neurofibromatosis 2 tumor suppressor and the yeast CDC42 gene. The method we describe
should be of general utility for the isolation of mutations in other X-linked genes, and should also provide an efficient
method for the isolation of new allcles of existing X-linked or autosomal mutations in Drosophila.
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| Biosis |
99548643 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
FBrf0094719 |
| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
| Page range |
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| Publisher |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
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Data from Reference
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| Aberrations (4)
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| Alleles (46)
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| Constructs (3)
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| Genes (10)
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