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Citation	Frise, E., Knoblich, J.A., Younger-Shepherd, S., Jan, L.Y., Jan, Y.N. (1996). The Drosophila Numb protein inhibits signaling of the Notch receptor during cell-cell interaction in sensory organ lineage. Proc. Natl. Acad. Sci. USA 93(21): 11925--11932.
FlyBase ID	FBrf0090560
Type of publication	Research paper
Offprint Available	No
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PubMed ID	8876239
PubMed Abstract	Specification of unequal daughter cell fates in the Drosophila external sense organ lineage requires asymmetric localization of the intrinsic determinant Numb as well as cell-cell interactions mediated by the Delta ligand and Notch receptor. Previous genetic studies indicated that numb acts upstream of Notch, and biochemical studies revealed that Numb can bind Notch. For a functional assay of the action of Numb on Notch signaling, we expressed these proteins in cultured Drosophila cells and used nuclear translocation of Suppressor of Hairless [Su(H)] as a reporter for Notch activity. We found that Numb interfered with the ability of Notch to cause nuclear translocation of Su(H); both the C-terminal half of the phosphotyrosine binding domain and the C terminus of Numb are required to inhibit Notch. Overexpression of Numb during wing development, which is sensitive to Notch dosage, revealed that Numb is also able to inhibit the Notch receptor in vivo. In the external sense organ lineage, the phosphotyrosine binding domain of Numb was found to be essential for the function but not for asymmetric localization of Numb. Our results suggest that Numb determines daughter cell fates in the external sense organ lineage by inhibiting Notch signaling.
Biosis	99261172
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Language of publication	English
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Abbreviation	Proc. Natl. Acad. Sci. USA
Title	Proceedings of the National Academy of Sciences of the United States of America
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Volume range	1-
Year range	1915-
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Place of publication	Washington, DC
Language of publication	English
ISBN/ISSN	0027-8424
CODEN	PNASA6
Data from Reference
Alleles (25)
	Dl^MtnA.PF Ecol\lacZ^MtnA.PF N^55e11 N^MGIIa.MtnA N^Scer\UAS.cSa N^int.MtnA Scer\FLP1^Scer\UAS.cBa Scer\GAL4^h-1J3 Scer\GAL4^ptc-559.1 Scer\GAL4^sca-109-68 Su(H)^{hs.T:Hsap\MYC} numb² numb^MtnA.PF numb^{Scer\UAS.T:Hsap\MYC} numb^Scer\UAS.cWa numb^hs.PF numb^{Δ1.hs.T:Hsap\MYC} numb^{Δ2.Scer\UAS.T:Hsap\MYC} numb^{Δ2.hs.T:Hsap\MYC} numb^{Δ3.hs.T:Hsap\MYC} numb^{Δ4.hs.T:Hsap\MYC} numb^{Δ5.hs.T:Hsap\MYC} numb^{ΔPTB.Scer\UAS.T:Hsap\MYC} numb^{ΔPTB.hs.T:Hsap\MYC} w^+mC
Constructs (6)
	P{UAS-FLP1.B} P{UAS-N.S} P{UAS-numb.T:Myc} P{UAS-numb.W} P{UAS-numbΔ2} P{UAS-numbΔPTB}
Genes (9)
	Dl Ecol\lacZ N Scer\FLP1 Scer\GAL4 Su(H) T:Hsap\MYC numb w
Insertions (3)
	P{GawB}h^1J3 P{GawB}ptc^559.1 P{GawB}sca^109-68