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Reference
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| Citation |
Stathakis, D.G., Pentz, E.S., Freeman, M.E., Kullman, J., Hankins, G.R., Pearlson, N.J., Wright, T.R.F. (1995). The genetic and molecular organization of the Dopa decarboxylase gene cluster of Drosophila melanogaster. Genetics 141(2): 629--655. |
| FlyBase ID |
FBrf0084402 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
8647399 |
| PubMed Abstract |
We report the complete molecular organization of the Dopa decarboxylase gene cluster. Mutagenesis screens recovered 77 new
Df(2L)TW130 recessive lethal mutations. These new alleles combined with 263 previously isolated mutations in the cluster to
define 18 essential genes. In addition, seven new deficiencies were isolated and characterized. Deficiency mapping, restriction
fragment length polymorphism (RFLP) analysis and P-element-mediated germline transformation experiments determined the gene
order for all 18 loci. Genomic and cDNA restriction endonuclease mapping, Northern blot analysis and DNA sequencing provided
information on exact gene location, mRNA size and transcriptional direction for most of these loci. In addition, this analysis
identified two transcription units that had not previously been identified by extensive mutagenesis screening. Most of the
loci are contained within two dense subclusters. We discuss the effectiveness of mutagens and strategies used in our screens,
the variable mutability of loci within the genome of Drosophila melanogaster, the cytological and molecular organization of
the Ddc gene cluster, the validity of the one band-one gene hypothesis and a possible purpose for the clustering of genes
in the Ddc region.
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| Biosis |
98498410 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Genetics |
| Title |
Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1916- |
| Page range |
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| Publisher |
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| Place of publication |
Austin, TX, etc |
| Language of publication |
English |
| ISBN/ISSN |
0016-6731 |
| CODEN |
GENTAE |
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Data from Reference
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| Aberrations (59)
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| Alleles (403)
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| Constructs (25)
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| Genes (26)
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| Insertions (1)
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