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Reference
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| Citation |
Pentz, E.S., Black, B.C., Wright, T.R. (1990). Mutations affecting phenol oxidase activity in Drosophila: quicksilver and tyrosinase-1. Biochem. Genet. 28(3/4): 151--171.
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| FlyBase ID |
FBrf0051216 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
2116788 |
| PubMed Abstract |
The complex enzyme phenol oxidase plays a major role in sclerotization and melanization of cuticle in insects. Production
of active enzyme from the inactive proenzyme involves at least six protein components in Drosophila. We examine here the biochemical
phenotype of two loci that affect phenol oxidase activity--quicksilver (qs; 1-39.5) and tyrosinase-1 (tyr-1; 2-54.5). Three
mutations isolated by different procedures in three different laboratories are alleles at the quicksilver locus. The effects
of these mutations have been monitored by means of enzyme assays in vitro and in polyacrylamide gels and by measurement of
catecholamine pool sizes. The activity of all three active enzyme components (A1, A2, and A3) is reduced in qs mutants. The
activated enzyme of one qs allele is thermolabile, while its activator is normal. Deletion and genetic mapping place tyr-1
near purple (pr; 2-54.5). Enzyme activity is reduced to 10% of normal but is not thermolabile and the activator is normal.
The activity of all three A components is reduced. The diphenol oxidase activity in double mutant combinations shows that
these mutations and Dox-A2 (Pentz et al., 1986) affect this enzyme in different ways.
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| Biosis |
90050626 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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| Associated files |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Biochem. Genet. |
| Title |
Biochemical Genetics |
| Authors |
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| Volume range |
1- |
| Year range |
1967- |
| Page range |
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| Publisher |
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| Place of publication |
New York |
| Language of publication |
English |
| ISBN/ISSN |
0006-2928 |
| CODEN |
BIGEBA |
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Data from Reference
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| Aberrations (9)
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| Alleles (5)
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| Genes (6)
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