Gene Dmel\crb

General Information
Symbol	Dmel\crb	Species	D. melanogaster
Name	crumbs	Annotation symbol	CG6383
Feature type	protein_coding_gene	FlyBase ID	FBgn0259685
Created / Updated	2008-05-29/2008-05-29
Genomic Location
Chromosome (arm)	3R	Recombination map	3-82
Cytogenetic map	95F10-95F11	Sequence location	3R:20,121,138..20,140,165 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene crumbs is referred to in FlyBase by the symbol crb (CG6383, FBgn0259685). It has the cytological map location 95F10-95F11. Its sequence location is 3R:20121138..20140165. Its molecular function is described as: calcium ion binding; spectrin binding; protein binding. It is involved in the biological processes described with 23 unique terms, many of which group under: anatomical structure development; establishment and/or maintenance of cell polarity; primary metabolic process; compound eye photoreceptor development; cell morphogenesis; intercellular junction assembly; positive regulation of cell proliferation; gamete generation; gland morphogenesis; epithelial cell differentiation; sensory organ development; zonula adherens maintenance; protein localization; membrane organization and biogenesis; establishment and/or maintenance of polarity of follicular epithelium; sensory perception of light stimulus. 68 alleles are reported. The phenotypes of these alleles are annotated with 38 unique terms, many of which group under: organ system; peripheral nervous system; nervous system; plasma membrane part; embryonic hindgut; spiracle; adult segment; plasma membrane; portion of tissue; integumentary system. It has 2 annotated transcripts and 2 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	crb: crumbs Homozygous lethal; many small holes in cuticle.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95F10-95F11 Limits computationally determined from genome sequence between P{PZ}crb⁰⁷²⁰⁷&P{PZ}BRWD3⁰⁵⁸⁴² and P{EP}CycB3^EP3127
Experimentally Determined Cytological Location
Cytogenetic map Notes References 95F3-95F10 (determined by in situ hybridisation) (Harden et al., 2002) 95F11-95F12 (determined by in situ hybridisation) (Spradling et al., 1999) 95F-95F (determined by in situ hybridisation) 95F3--10 (determined by in situ hybridisation) (Szeged Stock Center, 1998-) 95F9-95F13 (determined by in situ hybridisation) (Ranz et al., 1999) 95F-95F (determined by in situ hybridisation) (Deak et al., 1997) 95F11-95F12 (determined by in situ hybridisation) (Perrimon et al., 1996) 95F11-95F12 (BDGP Project Members, 1994-1999) 99F-99F (determined by in situ hybridisation) (Tepass et al., 1990) 95F-95F (determined by in situ hybridisation) (Knust et al., 1987)
Experimentally Determined Recombination Data
Location	3-82 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\crb for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) crb-RA FBtr0084603 NM_079756 7216 2146 crb-RB FBtr0111008 NM_001043286 7345 2189
Additional Transcript Data & Comments
Reported size (kB)	7.7, 7.5 (northern blot) (Tepass et al., 1990)
Comments	The exon/intron structure was roughy mapped. At least 4 introns are present in the primary transcript. (Tepass et al., 1990, Tepass et al., 1990)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein crb-PA FBpp0083987 233.6 2146 4.50 NP_524480 AAF56276 crb-PB FBpp0110307 238.6 2189 4.53 NP_001036751 ABI31202
Additional Polypeptide Data & Comments
Reported size (kD)	2139 (aa); 234 (kD) (Tepass et al., 1990)
Comments	Four Cys-poor regions of crb are distantly related to domains present in the C-terminal part of laminin-A chains and merosin. This homology suggests that the Cys-poor regions may be directly involved in its role in epithelial organization. (Wu and Lengyel, 1998) crb protein is used as a marker for the boundary cells that delineate the dorsal and ventral domains of the embryonic large intestine. (Maeda et al., 2007)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Aspartic acid and asparagine hydroxylation site (IPR000152) EGF-like, type 3 (IPR000742) EGF-like, type 2 (IPR001438) Laminin G (IPR001791) EGF-like calcium-binding (IPR001881) EGF-like (IPR006209) EGF (IPR006210) Concanavalin A-like lectin/glucanase (IPR008985) Laminin G, subdomain 1 (IPR012679) Laminin G, subdomain 2 (IPR012680) EGF-like region, conserved site (IPR013032) EGF calcium-binding (IPR013091) Concanavalin A-like lectin/glucanase, subgroup (IPR013320)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA439014 AA538865 AE003747 AAF56276 AQ026290 AQ073342 AW942272 AY118509 AAM49878 BH809473 BI165373 BP550115 BP550116 BP554271 BP558611 CL525925 CO185566 CO191688 CO267719 CO269501 CO286541 CO287178 CO296389 CO300419 CO306941 CO307197 CO308083 CO320855 CZ466902 CZ469513 CZ472564 CZ481877 CZ484322 CZ484323 CZ484341 CZ488604 CZ488605 EC060892 EC064272 EC064365 EC065893 EC066185 EC079887 EC081085 EC086262 EC208636 EC229736 EC233832 EC235272 EC245818 EC250614 M33753 AAA28428 X05144 CAA28793 Z71109

UniProtKB/Swiss-Prot	P10040
UniProtKB/TrEMBL

Maps to	126F3 (Ranz et al., 1999)
Does NOT map to
Identified with	LD13435 (BDGP Project Members, 2000-) Dm0334 (BDGP Project Members, 1994-1999, Spradling et al., 1999) D1400 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation crb[1] 3R:20,139,530..20,139,530 comment=wild-type protein. evidence=experimental na_change=C20139530T pr_change=Q2124@\|crb-PA reported_na_change=C6581T reported_pr_change=Q?@ (Wodarz et al., 1993)
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage-adult stage (Tepass et al., 1990) embryonic stage \| late adult salivary gland primordium (Myat and Andrew, 2002) embryonic stage (Tepass et al., 1990) embryonic stage embryonic/larval hindgut (Tepass et al., 1990)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference embryonic stage \| late adult salivary gland primordium (Myat and Andrew, 2002) larval stage \| third instar embryonic/larval garland cell (Tepass and Knust, 1990) adult stage \| male ejaculatory bulb (Tepass and Knust, 1990) embryonic stage ectoderm (Tepass et al., 1990) embryonic stage optic lobe (Tepass et al., 1990) embryonic stage \| stage 8-11 neuroblast (Tepass et al., 1990) pupal stage photoreceptor cell stalk \| apical (Nam and Choi, 2003) embryonic stage \| stage 16 hindgut (Wu and Lengyel, 1998) embryonic stage embryonic/larval salivary gland (Tepass et al., 1990) embryonic stage external sensory organ (Tepass et al., 1990) embryonic stage embryonic/larval foregut (Tepass et al., 1990) adult stage \| male ejaculatory duct (Tepass and Knust, 1990) embryonic stage embryonic peripheral nervous system (Tepass et al., 1990) adult stage \| male testicular duct (Tepass and Knust, 1990) larval stage \| third instar peripodial epithelium (Tepass and Knust, 1990) embryonic stage \| stage 8-11 stomodeum (Tepass et al., 1990) larval stage \| third instar salivary gland imaginal ring (Tepass and Knust, 1990) embryonic stage \| stage 8-11 posterior embryonic/larval midgut (Tepass et al., 1990) embryonic stage embryonic/larval hindgut (Tepass et al., 1990) embryonic stage Malpighian tubule (Tepass et al., 1990) embryonic stage epithelial cell (Tepass et al., 1990) adult stage \| male seminal vesicle (Tepass and Knust, 1990) oogenesis stage,adult stage follicle cell (Tepass and Knust, 1990) embryonic stage embryonic/larval trachea (Tepass et al., 1990) larval stage \| third instar imaginal disc (Tepass and Knust, 1990) embryonic stage stomatogastric nervous system (Tepass et al., 1990) embryonic stage endoderm (Tepass et al., 1990) embryonic stage chordotonal organ (Tepass et al., 1990) adult stage \| male male accessory gland (Tepass and Knust, 1990) larval stage \| third instar optic lobe (Tepass and Knust, 1990)
Marker for
	embryonic large intestine tracheal system ectodermally derived epithelia
Subcellular Localization
CV Term	plasma membrane (Tepass and Knust, 1990) plasma membrane \| apical (Tepass et al., 1990)
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable crb^EY06985 lethal crb^B-82 lethal (with Df(3R)crb87-4) crb^S050911 lethal (with Df(3R)crb87-5) crb^S010409 crb^S025807b crb^S050911 crb^S050920 crb^S058104 crb^S135506 crb^S138404 lethal \| recessive crb⁰⁷²⁰⁷ crb^07883b crb^D88-4 crb^D88-5 crb^D88-7 crb^D88-9 crb^R7 crb^S010409 crb^S025807b crb^S050911 crb^S050920 crb^S058104 crb^S135506 crb^S87-9 crb^j1B5 lethal \| embryonic stage crb^D88-3 lethal \| embryonic stage \| recessive crb¹ crb² crb^unspecified lethal \| embryonic stage, with Scer\GAL4^ptc-559.1 crb^Scer\UAS.cWa crb^{intra.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS} lethal \| embryonic stage, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa crb^{intra.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS} lethal \| larval stage \| recessive crb⁰⁷²⁰⁷ crb^D88-3 lethal \| pupal stage \| recessive crb^S010409 lethal, with Scer\GAL4^332.3 crb^Scer\UAS.cWa lethal, with Scer\GAL4^unspecified crb^Scer\UAS.cWa semi-lethal \| recessive crb^S138404 semi-lethal \| embryonic stage, with Scer\GAL4^en-e16E crb^{T6AT9AS11AS13A.intra.Scer\UAS} Other Phenotypes Allele wild-type crb^+t33 wild-type, with Scer\GAL4^GMR.PF crb^{IntraY10A.E16A.ΔERLI.Scer\UAS.T:Hsap\MYC} visible, with Scer\GAL4^en-e16E crb^{intra.Scer\UAS} visible, with Scer\GAL4^GMR.PF crb^{IntraY10A.E16A.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS} visible, with Scer\GAL4^sev.EP.M crb^{intra.Scer\UAS} cell shape defective, with Scer\GAL4^c381 crb^Scer\UAS.cWa cell polarity crb² cell polarity, with Scer\GAL4^en-e16E crb^{intra.Scer\UAS} cell polarity, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS.T:Hsap\MYC} cell polarity, with Scer\GAL4^unspecified crb^Scer\UAS.cWa hyperplastic, with Scer\GAL4^en-e16E crb^{intra.Scer\UAS} Sterility Allele female sterile crb⁰⁷²⁰⁷ fertile crb^EY06985 Phenotype manifest in Allele ovariole \| germ-line clone crb⁰⁷²⁰⁷ egg \| germ-line clone crb⁰⁷²⁰⁷ embryonic/first instar larval cuticle \| ventral crb⁰⁷²⁰⁷ larval salivary gland & embryo crb¹ crb² crb^D88-4 crb^D88-5 crb^D88-7 crb^D88-9 crb^R7 crb^S87-9 trachea crb¹ crb² crb^D88-4 crb^D88-5 crb^D88-7 crb^D88-9 crb^R7 crb^S87-9 embryonic epidermis crb¹ crb² amnioserosa crb¹ crb² crb^S010409 zonula adherens crb¹ crb² crb^D88-1 crb^unspecified spot adherens junction crb¹ crb² spiracular chamber crb¹ larval pharynx & embryo crb¹ larval esophagus & embryo crb¹ larval proventriculus & embryo crb¹ larval hindgut & embryo crb¹ crb^D88-4 crb^D88-5 crb^D88-7 crb^D88-9 crb^R7 crb^S87-9 embryonic Malpighian tubule crb¹ epithelial cell crb^11A2 crb^dsRNA.cLa ectoderm crb² follicle cell \| somatic clone crb² retina \| cell autonomous \| conditional qualifier \| somatic clone crb² rhabdomere \| cell autonomous \| somatic clone crb² crb^M82-4 photoreceptor cell stalk \| cell autonomous \| somatic clone crb² embryonic/first instar larval cuticle crb² crb^D88-1 crb^unspecified rhabdomere \| somatic clone crb² crb^D88-3 crb^S87-2 adherens junction \| somatic clone crb² crb^D88-3 photoreceptor cell stalk \| somatic clone crb² crb^D88-3 crb^S87-2 ommatidium \| somatic clone crb² interommatidial bristle \| somatic clone crb² zonula adherens & photoreceptor \| somatic clone crb² secondary pigment cell \| somatic clone crb² crb^S87-2 tertiary pigment cell \| somatic clone crb² crb^S87-2 adherens junction crb² adherens junction & stage 9 embryo crb² adherens junction & stage 10 embryo crb² epidermis crb^D88-2 crb^D88-4 crb^D88-5 crb^D88-7 crb^D88-9 crb^R7 crb^S87-2 crb^S87-9 embryonic/larval spiracle crb^D88-3 larval foregut & embryo crb^D88-4 crb^D88-5 crb^D88-7 crb^D88-9 crb^R7 crb^S87-9 embryonic epidermis, with Scer\GAL4^da.G32 crb^{Intra16-37.Scer\UAS.T:Rnor\CD2} crb^{IntraE16A.Scer\UAS.T:Hsap\MYC} crb^{IntraWT.Scer\UAS.T:Rnor\CD2} crb^{IntraY10A.E16A.Scer\UAS.T:Hsap\MYC} crb^{IntraY10A.Scer\UAS.T:Hsap\MYC} crb^Scer\UAS.cWa crb^{intra.Scer\UAS.T:Hsap\MYC} eye, with Scer\GAL4^GMR.PF crb^{IntraY10A.E16A.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} photoreceptor & adherens junction, with Scer\GAL4^GMR.PF crb^{IntraY10A.E16A.Scer\UAS.T:Hsap\MYC} crb^{intra.Scer\UAS.T:Hsap\MYC} embryonic hindgut crb^S010409 dorsal closure embryo crb^S010409 crb^S025807b crb^S025817 crb^S025819 crb^S050920 germ band crb^S010409 crb^S025807b crb^S025817 crb^S025819 crb^S050920 amnioserosa, with Scer\GAL4^332.3 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} embryonic/first instar larval cuticle, with Scer\GAL4^332.3 crb^Scer\UAS.cWa embryonic epidermis, with Scer\GAL4^332.3 crb^Scer\UAS.cWa dorsal closure embryo, with Scer\GAL4^c381 crb^Scer\UAS.cWa embryonic/first instar larval cuticle, with Scer\GAL4^c381 crb^Scer\UAS.cWa epidermis, with Scer\GAL4^ptc-559.1 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} amnioserosa, with Scer\GAL4^ptc-559.1 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} denticle belt, with Scer\GAL4^ptc-559.1 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} epidermis, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} tendon cell, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} crb^{intra.Scer\UAS.T:Hsap\MYC} zonula adherens, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} spot adherens junction, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} amnioserosa, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa crb^{intra.Scer\UAS} embryonic/first instar larval cuticle, with Scer\GAL4^da.G32 crb^Scer\UAS.cWa photoreceptor cell stalk, with Scer\GAL4^ninaE.PHS crb^Scer\UAS.cWa crb^{extra.Scer\UAS} crb^{extraTM.Scer\UAS.T:Avic\GFP} embryonic salivary gland, with Scer\GAL4^fkh.PH crb^Scer\UAS.cWa eye disc, with Scer\GAL4^unspecified crb^Scer\UAS.cWa eye, with Scer\GAL4^unspecified crb^Scer\UAS.cWa posterior crossvein, with Scer\GAL4^en-e16E crb^{T6AT9AS11AS13A.intra.Scer\UAS} embryonic/larval spiracle, with Scer\GAL4^ems.HRE crb^{T6AT9AS11AS13A.intra.Scer\UAS} dorsal mesothoracic disc & epithelial cell, with Scer\GAL4^en-e16E crb^{T6DT9D.intra.Scer\UAS} crb^{intra.Scer\UAS} ommatidium, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS} interommatidial bristle, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS} ommatidial cluster, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS} secondary pigment cell, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS} tertiary pigment cell, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS} eye disc, with Scer\GAL4^GMR.PF crb^{intra.Scer\UAS} eye, with Scer\GAL4^sev.EP.M crb^{intra.Scer\UAS} ommatidium, with Scer\GAL4^sev.EP.M crb^{intra.Scer\UAS} interommatidial bristle, with Scer\GAL4^sev.EP.M crb^{intra.Scer\UAS} wing, with Scer\GAL4^en-e16E crb^{intra.Scer\UAS} dorsal mesothoracic disc \| posterior compartment, with Scer\GAL4^en-e16E crb^{intra.Scer\UAS}