Gene Dmel\AGO3

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General Information

Symbol

Dmel\AGO3

Species

D. melanogaster

Name

Argonaute 3

Annotation symbol

CG40300

Feature type

protein_coding_gene

FlyBase ID

FBgn0250816

Created / Updated

2008-02-21/2008-02-21

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

Sequence location

3L:23,547,715..23,682,722 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Argonaute 3 is referred to in FlyBase by the symbol AGO3 (CG40300, FBgn0250816). Its sequence location is 3L:23547715..23682722. Its molecular function is described as RNA binding. The biological processes in which it is involved are not known. No alleles are reported. It has 3 annotated transcripts and 3 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

80-80

h47

(Drosophila Heterochromatin Genome Project, 2004)

80B-80C

(determined by in situ hybridisation)

(Moshkin et al., 2002)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\AGO3 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

AGO3-RD

FBtr0299880

2718

867

AGO3-RE

FBtr0299881

2680

867

AGO3-RF

FBtr0299882

2733

867

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

AGO3-PD

FBpp0289158

99.1

867

9.04

AGO3-PE

FBpp0289159

99.1

867

9.04

AGO3-PF

FBpp0289160

99.1

867

9.04

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Argonaute and Dicer protein, PAZ (IPR003100)

Stem cell self-renewal protein Piwi (IPR003165)

Polynucleotidyl transferase, Ribonuclease H fold (IPR012337)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7PLK0

Maps to

Does NOT map to

Identified with

LD15785

(Moshkin et al., 2002)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of AGO3	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 0 )

For All Alleles Carried on Transgenic Constructs Show

Allele of AGO3	Class	Mutagen	Stocks	Known lesion

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 2 )

Molecular Function

CV term

Evidence

References

RNA binding

inferred from direct assay

(Brennecke et al., 2007)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Gunawardane et al., 2007)

inferred from direct assay

(Gunawardane et al., 2007)

inferred from direct assay

(Brennecke et al., 2007)

Sequence Ontology: Class of Gene

gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

AGO3 allele

Gene

References

External Data

Linkouts

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 2 )

cDNA Clones ( 24 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH03753

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

EP19658

RNAi & Array Information

Affy Oligo

Linkouts

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: AGO3 CG40300

(Gunawardane et al., 2007)

Source for merge of: AGO3 BEST:LD15785

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

AGO3 protein exhibits Slicer activity in vitro.

(Gunawardane et al., 2007)

AGO3 protein binds sense-strand piRNAs.

(Brennecke et al., 2007)

Other Comments

New annotation (CG40300) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

External Crossreferences & Linkouts

Sequence Crossreferences

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q7PLK0

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Argonaute and Dicer protein, PAZ (IPR003100)

Stem cell self-renewal protein Piwi (IPR003165)

Polynucleotidyl transferase, Ribonuclease H fold (IPR012337)

Linkouts

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/genebrief/ago3.htm

Synonyms & Secondary IDs ( 9 )

Reported As

Symbol Synonym

Ago3

(Zamore, 2007, Zaratiegui, 2007, Lin, 2007, Brennecke et al., 2007)

AGO3

(Grewal and Elgin, 2007, Gunawardane et al., 2007, Gunawardane et al., 2007)

ago3

(Vagin et al., 2006)

Ago-3

(Klenov, 2008)

BEST:LD15785

CG40300

(Gunawardane et al., 2007, )

dAgo3

(Denli and Hannon, 2003)

Name Synonym

AGO3

Argonaute3

(Zamore, 2007)

Argonaute 3

BEST:LD15785

CG40300

Secondary FlyBase IDs

FBgn0086780
FBgn0066316
FBgn0058300
FBan0040300
FBgn0069062

References ( 17 )

Research paper

Brennecke et al., 2007, Cell 128(6): 1089--1103: Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. [FBrf0200494]
Gunawardane et al., 2007, Science 315(5818): 1587--1590: A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila. [FBrf0194433]
Vagin et al., 2006, Science 313(5785): 320--324: A distinct small RNA pathway silences selfish genetic elements in the germline. [FBrf0194520]
Moshkin et al., 2002, Chromosoma 111(2): 114--125: Microdissection and sequence analysis of pericentric heterochromatin from the Drosophila melanogaster mutant Suppressor of Underreplication. [FBrf0151235]
Williams and Rubin, 2002, Proc. Natl. Acad. Sci. USA 99(10): 6889--6894: ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. [FBrf0149162]

Supplementary material

Gunawardane et al., 2007, Supporting online material.: Supporting online material. [FBrf0200143]

Review

Grewal and Elgin, 2007, Nature 447(7143): 399--406: Transcription and RNA interference in the formation of heterochromatin. [FBrf0200338]
Lin, 2007, Science 316(5823): 397: piRNAs in the germ line. [FBrf0200130]
Zamore, 2007, Nature 446(7138): 864--865: RNA silencing: genomic defence with a slice of pi. [FBrf0200335]
Zaratiegui, 2007, Cell 128(4): 736--776: [FBrf0198665]
Kavi et al., 2005, FEBS Lett. 579(26): 5940--5949: RNA silencing in Drosophila. [FBrf0190485]
Denli and Hannon, 2003, Trends Biochem. Sci. 28(28): 196--201: RNAi: an ever-growing puzzle. [FBrf0182729]

Personal communication to FlyBase

Drosophila Heterochromatin Genome Project, 2006, Release 5.1 of the annotated Drosophila melanogaster heterochromatin.: Release 5.1 of the annotated Drosophila melanogaster heterochromatin. [FBrf0188763]
Drosophila Heterochromatin Genome Project, 2004, Release 3.2 of the annotated Drosophila melanogaster heterochromatin.: Release 3.2 of the annotated Drosophila melanogaster heterochromatin. [FBrf0173307]

Abstract

Klenov, 2008, A. Dros. Res. Conf. 49: 896B: The role of Drosophila fmr1 gene in rasiRNA silencing. [FBrf0203619]

FlyBase analysis

FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome.: Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]