Gene Dmel\Ctr1B

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General Information

Symbol

Dmel\Ctr1B

Species

D. melanogaster

Name

Copper transporter 1B

Annotation symbol

CG7459

Feature type

protein_coding_gene

FlyBase ID

FBgn0062412

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

84F11-84F11

Sequence location

3R:4,143,340..4,145,285 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Copper transporter 1B is referred to in FlyBase by the symbol Ctr1B (CG7459, FBgn0062412). It has the cytological map location 84F11. Its sequence location is 3R:4143340..4145285. Its molecular function is described as: copper ion transmembrane transporter activity; copper uptake transmembrane transporter activity. It is involved in the biological processes: copper ion transport; response to metal ion. 12 alleles are reported. The phenotypes of these alleles are annotated with: adult cuticle; eye. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84F11-84F11

Limits computationally determined from genome sequence between P{EP}EP833 and P{lacW}l(3)L4740^L4740

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Ctr1B for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Ctr1B-RA

FBtr0081835

NM_141533

1305

174

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Ctr1B-PA

FBpp0081325

19.8

174

7.34

NP_649790

AAF54223

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ctr copper transporter (IPR007274)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VHS6

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8614

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0062412

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Ctr1B

Stage(s) 4-6

Stage(s) 7-8

Stage(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | conditional qualifier (with Df(3R)p40)

Ctr1B^3-4

lethal | conditional qualifier | recessive

Ctr1B^3-4

lethal, with Scer\GAL4^Act5C

Ctr1B^Scer\UAS.cSa

Other Phenotypes

Allele

visible | conditional qualifier, with Scer\GAL4^GMR.PF

Ctr1B^Scer\UAS.cSa

body color defective | conditional qualifier

Ctr1B^3-4

chemical sensitive

Ctr1B^3-4

Phenotype manifest in

Allele

adult cuticle | conditional qualifier

Ctr1B^3-4

eye | conditional qualifier, with Scer\GAL4^GMR.PF

Ctr1B^Scer\UAS.cSa

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of Ctr1B	Mutagen	Stocks	Known lesion
Ctr1B^11-19	P-element activity	0	Yes
Ctr1B^3-4	Δ2-3	0	Yes
Ctr1B^e01412	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Ctr1B	Mutagen	Stocks	Known lesion
Ctr1B^{AH1.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion in vitro construct \| genomic fragment in vitro construct \| deletion	0	Yes
Ctr1B^{AH2.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion in vitro construct \| genomic fragment in vitro construct \| deletion	0	Yes
Ctr1B^{AH3.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion in vitro construct \| genomic fragment in vitro construct \| deletion	0	Yes
Ctr1B^Act5C.PZ	in vitro construct \| regulatory fusion	0	Yes
Ctr1B^GD2859	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Ctr1B^Scer\UAS.cSa	in vitro construct \| regulatory fusion	0	Yes
Ctr1B^{m1-2-3-4.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion in vitro construct \| site directed mutagenesis in vitro construct \| genomic fragment	0	Yes
Ctr1B^{m1-m2-3-4.T:Avic\GFP-EGFP}	in vitro construct \| coding region fusion in vitro construct \| site directed mutagenesis in vitro construct \| genomic fragment	0	Yes
Ctr1B^{m1-m2-m3-m4.T:Avic\GFP-EGFP}	in vitro construct \| site directed mutagenesis in vitro construct \| genomic fragment in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)CA3

Df(3R)p40

Useful duplication

Dp(3;3)D1

Disrupted in

Df(3R)Exel6147

(Ryder, 2004.4.26)

Df(3R)p40

(Zhou et al., 2003)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD2859}

P{UAS-Ctr1B.S}

characterization construct

P{Ctr1B-EGFP.AH1}

P{Ctr1B-EGFP.AH3}

P{Ctr1B-EGFP.m1-2-3-4}

P{Ctr1B-EGFP.m1-m2-3-4}

P{Ctr1B-mMRE1-mMRE2-mMRE3-mMRE4.EGFP}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}Ctr1B^e01412

insertion of mobile activating element

P{EP}EP833

Related Comments

Please look at the allele reports for the complete phenotype data

S2 cells treated with Ctr1B dsRNA show significantly reduced copper uptake from the culture medium.

(Southon et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

copper ion transmembrane transporter activity

non-traceable author statement

(Zhou et al., 2002)

inferred from direct assay

(Zhou et al., 2003)

inferred from sequence or structural similarity with NCBI_gi:4507015

(FlyBase, 1992-)

copper uptake transmembrane transporter activity

inferred from mutant phenotype

(Southon et al., 2004)

Biological Process

CV term

Evidence

References

copper ion transport

inferred from direct assay

(Zhou et al., 2003)

response to metal ion

inferred from mutant phenotype

(Selvaraj et al., 2005)

Cellular Component

CV term

Evidence

References

integral to membrane

inferred from electronic annotation with InterPro:IPR007274

(FlyBase Curators et al., 2004-)

plasma membrane

non-traceable author statement

(Zhou et al., 2002)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Ctr1B allele

Gene

References

unspecified

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66170

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18342

Dere\GG25028

Dgri\GH14206

Dmoj\GI24869

Dper\GL23430

Dpse\GA20368

Dsec\GM23723

Dsim\GD15144

Dvir\GJ24513

Dwil\GK13179

Dyak\GE25868

Curated drosophilid orthologs

Dpse\GA20368

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

Q69P80
Oryza sativa (Gramene)
DDB0191340
Dictyostelium discoideum (Dictybase)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

v5804

w¹¹¹⁸; P{GD2859}v5804

v5805

w¹¹¹⁸; P{GD2859}v5805

Genomic Clones ( 1 )

BACR33N15

cDNA Clones ( 14 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE11491

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0062412

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Ctr1B CG7459

(Zhou et al., 2003)

Additional comments

Comments About Role

Regulation of Ctr1B upon copper starvation is lost in the absence of MTF-1.

(Selvaraj et al., 2005)

Comments About Molecular Function

Metal Response Elements (MREs), upstream of Ctr1B are critical for the up-regulation of Ctr1B transcription under copper-limiting conditions.

(Selvaraj et al., 2005)

Other Comments

dsRNA made from templates generated with primers directed against this gene used to investigate the role of this gene in copper homoeostasis in S2 cells.

(Southon et al., 2004)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_141533

RefSeq (Proteins)

•

NP_649790

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q9VHS6

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ctr copper transporter (IPR007274)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66170

DEDB - Drosophila exon database: splicing graphs

•

8614

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0062412

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0062412

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0062412

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0062412

InParanoid orthologs - Eukaryotic orthologs

•

Q69P80
Oryza sativa (Gramene)
DDB0191340
Dictyostelium discoideum (Dictybase)

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

CG7459

(Zhou et al., 2003, )

Ctr1B

(Egli et al., 2006, Egli et al., 2006, Hua et al., 2008, Yepiskoposyan et al., 2006, Turski and Thiele, 2007)

Name Synonym

Copper transporter 1B

(Zhou et al., 2002)

Secondary FlyBase IDs

FBgn0037575

References ( 20 )

Research paper

Turski and Thiele, 2007, J. Biol. Chem. 282(33): 24017--24026: Drosophila Ctr1A functions as a copper transporter essential for development. [FBrf0201727]
Egli et al., 2006, Molec. Cell. Biol. 26(6): 2286--2296: A family knockout of all four Drosophila metallothioneins reveals a central role in copper homeostasis and detoxification. [FBrf0191190]
Egli et al., 2006, Genes to Cells 11(6): 647--658: The four members of the Drosophila metallothionein family exhibit distinct yet overlapping roles in heavy metal homeostasis and detoxification. [FBrf0194402]
Yepiskoposyan et al., 2006, Nucleic Acids Res. 34(17): 4866--4877: Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc. [FBrf0193346]
Selvaraj et al., 2005, Genes Dev. 19(8): 891--896: Metal-responsive transcription factor (MTF-1) handles both extremes, copper load and copper starvation, by activating different genes. [FBrf0187596]
Southon et al., 2004, Biochem. J. 383(2): 303--309: Copper homoeostasis in Drosophila melanogaster S2 cells. [FBrf0179972]
Zhou et al., 2003, J. Biol. Chem. 278(48): 48210--48218: A copper-regulated transporter required for copper acquisition, pigmentation, and specific stages of development in Drosophila melanogaster. [FBrf0167752]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

Abstract

Hua et al., 2008, A. Dros. Res. Conf. 49: 368B: Metal sensing mechanism of metal-responsive transcription factor 1 (MTF-1). [FBrf0204011]
Turski et al., 2005, A. Dros. Res. Conf. 46: 261C: Analysis of Drosophila Copper Transporters: The Essential Role of Copper in Development. [FBrf0183575]
Yepiskoposyan et al., 2005, A. Dros. Res. Conf. 46: 377B: Transcriptional regulation of metal homeostasis. [FBrf0183670]
Zhou et al., 2003, A. Dros. Res. Conf. 44: 270C: The role of the essential nutrient copper in Drosophila development: function and transcriptional regulation of the Ctr1B copper transporter. [FBrf0154433]
Zhou et al., 2002, A. Dros. Res. Conf. 43 Suppl.: 1009A: Understanding Copper Homeostasis in Drosophila Physiology and Development. [FBrf0146685]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.: Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Curated genome annotation

Ketchum, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126677]