Gene Dmel\CG32666

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General Information

Symbol

Dmel\CG32666

Species

D. melanogaster

Name

CG32666

Annotation symbol

CG32666

Feature type

protein_coding_gene

FlyBase ID

FBgn0052666

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

10C1-10C5

Sequence location

X:11,387,203..11,449,079 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG32666 (FBgn0052666). It has the cytological map location 10C1-10C5. Its sequence location is X:11387203..11449079. Its molecular function is described as: protein serine/threonine kinase activity; ATP binding. It is involved in the biological processes: protein amino acid phosphorylation; cell adhesion; regulation of cell shape. 10 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

10C1-10C5

Limits computationally determined from genome sequence between P{EP}CG11756^EP1610 and P{EP}CG32666^EP1452

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CG32666 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG32666-RA

FBtr0073508

NM_206688

4216

784

CG32666-RB

FBtr0073507

NM_167288

4404

784

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG32666-PA

FBpp0073357

85.7

784

6.64

NP_996411

AAS65317

CG32666-PB

FBpp0073356

85.7

784

6.64

NP_727533

AAF48053

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0052666

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, core (IPR000719)

Serine/threonine protein kinase (IPR002290)

Serine/threonine protein kinase, active site (IPR008271)

Protein kinase-like (IPR011009)

Serine/threonine protein kinase-related (IPR017442)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

RE12147

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2513

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI404003
AI064495

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0052666

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0052666

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0052666

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of CG32666	Mutagen	Stocks	Known lesion
CG32666^BG00876	P-element activity	1	--
CG32666^EP1452	P-element activity	2	--
CG32666^KG03058	P-element activity	1	--
CG32666^MB05079		1	--
CG32666^d06322	P-element activity	1	--
CG32666^e02427	piggyBac transposase	1	--
CG32666^e03054	piggyBac transposase	1	--
CG32666^f08006	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG32666	Class	Mutagen	Stocks	Known lesion
CG32666^GD6671		in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
CG32666^GD9422		in vitro construct \| RNAi in vitro construct \| regulatory fusion	3	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)N71

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6671}

P{GD9422}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}CG32666^e02427

PBac{RB}CG32666^e03054

PBac{WH}CG32666^f08006

P{GT1}BG01732

P{GT1}CG32666^BG00876

P{SUPor-P}CG32666^KG03058

insertion of enhancer trap binary system

Mi{ET1}CG32666^MB05079

insertion of mobile activating element

P{EP}CG32666^EP1452

P{XP}CG32666^d06322

Related Comments

Please look at the allele reports for the complete phenotype data

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: cells become round and detached.

(Kiger et al., 2003)

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017442

(FlyBase Curators et al., 2004-)

protein serine/threonine kinase activity

non-traceable author statement

(Morrison et al., 2000)

inferred from electronic annotation with InterPro:IPR002290, InterPro:IPR008271

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

cell adhesion

inferred from mutant phenotype

(Kiger et al., 2003)

protein amino acid phosphorylation

non-traceable author statement

(Morrison et al., 2000)

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008271, InterPro:IPR017442

(FlyBase Curators et al., 2004-)

regulation of cell shape

inferred from mutant phenotype

(Kiger et al., 2003)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG32666 allele

Gene

References

CG32666^EP1452

pnr

(Pena-Rangel et al., 2002)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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58507

Orthologs

Genome-wide drosophilid orthologs

Dana\GF19157

Dere\GG18425

Dgri\GH12374

Dmoj\GI14753

Dper\GL19896

Dpse\GA17051

Dsec\GM13087

Dvir\GJ19156

Dwil\GK16306

Dyak\GE15943

Curated drosophilid orthologs

Dpse\GA17051

Dsim\CG32666

(Orengo, 2004.6.4)

Linkouts

InParanoid orthologs - Eukaryotic orthologs

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ENSANGP00000011924
Anopheles gambiae (Ensembl)
ENSBTAP00000017564
Bos taurus (Ensembl)
ENSCAFP00000015677
Canis familiaris (Ensembl)
NEWSINFRUP00000127908
Takifugu rubripes (Ensembl)
ENSGALP00000020150
Gallus gallus (Ensembl)
ENSP00000319192
Homo sapiens (Ensembl)
ENSMMUP00000025013
Macaca mulatta (Ensembl)
ENSMODP00000015177
Monodelphis domestica (Ensembl)
ENSPTRP00000032664
Pan troglodytes (Ensembl)
ENSRNOP00000016857
Rattus norvegicus (Ensembl)
GSTENP00022038001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000034480
Xenopus tropicalis (Ensembl)
ZDB-CDNA-040425-2433
Danio rerio (Zfin)
ZDB-GENE-040426-1499
Danio rerio (Zfin)
MGI:2138162
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 13 )

PBac{RB}CG32666^e02427

PBac{RB}CG32666^e03054

PBac{WH}CG32666^f08006

Bloomington

12564

w¹¹¹⁸ P{GT1}BG01732

11300

w¹¹¹⁸ P{EP}CG32666^EP1452

12443

w¹¹¹⁸ P{GT1}CG32666^BG00876

24605

w¹¹¹⁸ Mi{ET1}CG32666^MB05079

VDRC

v32960

w¹¹¹⁸; P{GD9422}v32960

v32961

w¹¹¹⁸; P{GD9422}v32961/TM3

v44374

w¹¹¹⁸; P{GD9422}v44374/TM3

More stocks available...

Genomic Clones ( 2 )

cDNA Clones ( 29 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE12147

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

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FBgn0052666

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

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FBgn0052666

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG11713 CG1760 Annotations CG11713, CG1760 merged as CG32666 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: CG32666 EP1452 Source for merge of CG32666 EP1452 was that EP1452 insertion is within CG32666 (and in the right orientation) (date:040519).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)