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Gene Dmel\CG32549

General Information
Symbol	Dmel\CG32549	Species	D. melanogaster
Name	CG32549	Annotation symbol	CG32549
Feature type	protein_coding_gene	FlyBase ID	FBgn0052549
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	17A10-17A11	Sequence location	X:18,284,521..18,304,168 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol CG32549 (FBgn0052549). It has the cytological map location 17A10-17A11. Its sequence location is X:18284521..18304168. Its molecular function is described by 5'-nucleotidase activity. It is involved in the biological process nucleobase, nucleoside, nucleotide and nucleic acid metabolic process. 15 alleles are reported. No phenotypic data is available. It has 6 annotated transcripts and 6 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 17A10-17A11   Limits computationally determined from genome sequence between P{EP}EP1378 and P{EP}CG32549EP1317
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\CG32549 for information on other features
Comments on Gene Model
	DGC clone GH10029 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG32549-RA FBtr0074573  NM_206787   2865   715 CG32549-RB FBtr0074574  NM_206786   2782   612 CG32549-RC FBtr0074572  NM_167615   1896   508 CG32549-RD FBtr0074570  NM_133061   2311   581 CG32549-RE FBtr0074571  NM_167614   2548   630 CG32549-RF FBtr0074575  NM_206785   2653   612
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein CG32549-PA   FBpp0074345   81.6   715   7.53     NP_996510   AAS65404 CG32549-PB   FBpp0074346   69.8   612   6.07     NP_996509   AAS65405 CG32549-PC   FBpp0074344   57.7   508   6.20     NP_728163   AAF48832 CG32549-PD   FBpp0074342   66.2   581   6.47     NP_573289   AAF48835 CG32549-PE   FBpp0074343   71.7   630   6.58     NP_728162   AAF48833 CG32549-PF   FBpp0074347   69.8   612   6.07     NP_996508   AAS65406
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0052549
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase (IPR008380) Purine 5'-nucleotidase (IPR016695)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697748   AA698832   AA698921   AA803936   AE003508 AAF48832   AE003508 AAF48833   AE003508 AAF48835   AE003508 AAS65404   AE003508 AAS65405   AE003508 AAS65406   AI110090   AI113635   AI296606   AI403205   AI404443   AI517397   AI517732   AJ783236   AJ783237   AJ783238   AJ783239   AJ783240   AJ783241   AJ783242   AJ783243   AJ783244   AJ783245   AJ783246   AJ783247   AJ783248   AW940345   AY051424 AAK92848   BF485918   BF487490   BF501828   BF505798   BG640357   BG640706   BI168346   BI212221   BI229904   BI236015   BI242269   BI373215   BI375024   BI565827   BI578698   BI611428   BI617739   BI619402   BI621361   BI622313   BI623345   BI624651   BI627963   CA803269   CA803411   CA804634   CA806131   CA806917   CK658095   CO274780   CO284096   CO284739   CO304503   CO308328   CO313974   CO331521   CZ467119   CZ471823   CZ472155   CZ472549   CZ474035   CZ477746   CZ481122   CZ488762   CZ490522   EC067471   EC195827   EC223146   EC225768   EC239619   EC243846   EC247824   EC248634   EL873229
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KUW2 Q7KUW3 Q9VWV2 Q9VWV4 Q9VWV5
Maps to
Does NOT map to
Identified with	GH10029 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 3239
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI110090 AI113635
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0052549 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0052549 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0052549 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG32549 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG32549EY16248 Sterility Allele fertile CG32549EY16248 Phenotype manifest in Allele
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of CG32549 Class Mutagen Stocks Known lesion CG32549EP1317 P-element activity 2 -- CG32549EY16248 P-element activity 1 -- CG32549c01384 piggyBac transposase 1 -- CG32549d00819 P-element activity 1 -- CG32549d01316 P-element activity 0 -- CG32549d01985 P-element activity 0 -- CG32549d04625 P-element activity 1 -- CG32549d11468 P-element activity 0 -- CG32549e03350 piggyBac transposase 1 -- CG32549f06115 piggyBac transposase 1 -- CG32549f07786 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG32549 Class Mutagen Stocks Known lesion CG32549GD13708 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes CG32549GD14528 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes CG32549GD14594 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes CG32549GD6515 in vitro construct | RNAi in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)N19
Useful duplication	Dp(1;1)Bxr49k
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13708} NA P{GD14528} NA P{GD14594} NA P{GD6515} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}CG32549c01384 NA PBac{RB}CG32549e03350 NA PBac{WH}CG32549f06115 NA PBac{WH}CG32549f07786 NA insertion of mobile activating element P{EPgy2}CG32549EY16248 NA P{EP}CG32549EP1317 NA P{XP}CG32549d00819 NA P{XP}CG32549d01316 NA P{XP}CG32549d01985 NA P{XP}CG32549d04625 NA P{XP}CG32549d11468 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 3 )
Molecular Function
CV term Evidence References
5'-nucleotidase activity inferred from sequence or structural similarity with EMBL:U73690 (FlyBase, 1992-)
Biological Process
CV term Evidence References
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process inferred from sequence or structural similarity with EMBL:U73690 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
cytosol inferred from sequence or structural similarity with EMBL:U73690 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG32549 allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 59139
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22508 Dere\GG18126 Dgri\GH12351 Dmoj\GI15598 Dper\GL27036 Dpse\GA22323 Dsec\GM22819 Dsim\GD15638 Dvir\GJ15201 Dwil\GK25265 Dyak\GE15532
Curated drosophilid orthologs	Dpse\GA16980   Dsim\GD15640 (Orengo, 2004.6.4)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000010003 Anopheles gambiae (Ensembl) ENSBTAP00000017090 Bos taurus (Ensembl) ENSCAFP00000015235 Canis familiaris (Ensembl) ENSCINP00000015119 Ciona intestinalis (Ensembl) NEWSINFRUP00000134882 Takifugu rubripes (Ensembl) ENSGALP00000013294 Gallus gallus (Ensembl) ENSP00000339479 Homo sapiens (Ensembl) ENSMMUP00000005000 Macaca mulatta (Ensembl) ENSMODP00000013955 Monodelphis domestica (Ensembl) ENSPTRP00000005117 Pan troglodytes (Ensembl) ENSRNOP00000027351 Rattus norvegicus (Ensembl) GSTENP00022699001 Tetraodon nigroviridis (Ensembl) ENSXETP00000041119 Xenopus tropicalis (Ensembl) Q7XZV8 Oryza sativa (Gramene) AT1G75210.1 Arabidopsis thaliana (Arabidopsis) WBGene00025181 Caenorhabditis briggsae (Wormbase) WBGene00022201 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040912-15 Danio rerio (Zfin) ZDB-GENE-040912-13 Danio rerio (Zfin) DDB0230202 Dictyostelium discoideum (Dictybase) MGI:2178563 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Harvard	- P{XP}CG32549d00819 - P{XP}CG32549d04625 - PBac{PB}CG32549c01384 - PBac{RB}CG32549e03350 - PBac{WH}CG32549f06115
Szeged	- P{EP}CG32549EP1317
VDRC	v42260 w1118 P{GD14528}v42260 v23597 w1118; P{GD13708}v23597 v23598 w1118; P{GD13708}v23598 v30079 w1118; P{GD14594}v30079 v42258 w1118; P{GD14528}v42258 v30080 w1118; P{GD14594}v30080/CyO
	More stocks available...
Genomic Clones ( 2 )
	BACR10E05 BACR03K10