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Gene Dmel\mir-1

General Information
Symbol	Dmel\mir-1	Species	D. melanogaster
Name	mir-1	Annotation symbol	CR32958
Feature type	miRNA_gene	FlyBase ID	FBgn0046834
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	38C8-38C8	Sequence location	2L:20,487,496..20,487,517 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol mir-1 (CR32958, FBgn0046834). It has the cytological map location 38C8. Its sequence location is 2L:20487496..20487517. Its molecular function is unknown. It is involved in the biological processes: cardiac cell differentiation; regulation of Notch signaling pathway. 9 alleles are reported. The phenotypes of these alleles are annotated with: cardioblast; embryonic/larval somatic muscle; abdominal 7 dorsal acute muscle 1; mesothoracic dorsal acute muscle 1; metathoracic dorsal acute muscle 1. It has one annotated transcript.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38C8-38C8   Limits computationally determined from genome sequence between P{EP}CG16798EP401&P{lacW}k07219 and P{lacW}k02501&P{lacW}k14810
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\mir-1 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt)   mir-1-RA FBtr0081379  NR_001675   22
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences	miRBase - the home of microRNA data • MI0000116
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence   Name AJ421756
Maps to
Does NOT map to
Identified with
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage-adult stage   (Sempere et al., 2003)
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0046834
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive | second instar larval stage mir-1KO lethal | embryonic stage mir-1Δ31kb lethal | embryonic stage, with Scer\GAL4how-24B mir-1Scer\UAS.cKa lethal | embryonic stage, with Scer\GAL4da.G32 mir-1Scer\UAS.cKa lethal | larval stage mir-1Δ31kb Other Phenotypes Allele hypoactive | larval stage | recessive mir-1KO Phenotype manifest in Allele visceral muscle of larval heart & embryonic/larval pericardial cell mir-1Δ31kb cardioblast mir-1Δ31kb embryonic/larval somatic muscle mir-1Δ31kb abdominal 7 dorsal acute muscle 1 mir-1Δ31kb mesothoracic dorsal acute muscle 1 mir-1Δ31kb metathoracic dorsal acute muscle 1 mir-1Δ31kb larval somatic muscle & second instar larva mir-1KO cardioblast, with Scer\GAL4da.G32 mir-1Scer\UAS.cKa embryonic/larval somatic muscle, with Scer\GAL4da.G32 mir-1Scer\UAS.cKa cardioblast, with Scer\GAL4how-24B mir-1Scer\UAS.cKa embryonic/larval somatic muscle, with Scer\GAL4how-24B mir-1Scer\UAS.cKa
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of mir-1 Class Mutagen Stocks Known lesion mir-1KO FLPase SCEI endonuclease recombination I-CreI endonuclease 0 Yes mir-1Δ31kb FLPase 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mir-1 Class Mutagen Stocks Known lesion mir-1+t8.6 in vitro construct | genomic fragment 0 Yes mir-18.6.XhoI in vitro construct | site directed mutagenesis 0 Yes mir-1KO.Scer\SceI.RS in vitro construct | other 0 Yes mir-1Scer\UAS.P\T.cSa in vitro construct | regulatory fusion 0 Yes mir-1Scer\UAS.T:Disc\RFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes mir-1Scer\UAS.cKa in vitro construct | regulatory fusion in vitro construct | genomic fragment 0 Yes mir-1αTub84B.PS in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)pr76
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{mir-1-lacZ.E1-5} No P{mir-1-lacZ.E1-7} No P{mir-1-lacZ.E2-5} No P{mir-1-lacZ.H-Pelican} No P{mir-1.eve-lacZ} No UAS construct P{UAS-mir-1.K} NA P{UASp-mir-1.S} NA characterization construct P{FRT(w+.mir-1KO.Scer\SceI.RS)} NA P{mir-1+t8.6} NA P{mir-18.6.XhoI} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 2 )
Molecular Function
CV term Evidence References
Biological Process
CV term Evidence References
cardiac cell differentiation inferred from mutant phenotype (Kwon et al., 2005)
regulation of Notch signaling pathway inferred from mutant phenotype (Kwon et al., 2005)
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	miRNA_gene (Lagos-Quintana et al., 2001) nuclear_gene (Lagos-Quintana et al., 2001)
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mir-1 allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 534310
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\mir-1
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 3 )
	BACR03G24 BACR08D17 BACR34H03
cDNA Clones ( 0 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Affy Oligo
Linkouts	GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0046834
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
	mir-1 is not required for the formation or physiological function of the larval musculature, but is required for the dramatic post-mitotic growth of larval muscle. (Sokol and Ambros, 2005)
Comments About Molecular Function
	Tiling experiments indicate that the mir-1 gene is 2.9kb in length. mir-1 is directly activated by dl amd twi. (Biemar et al., 2005)
Other Comments
	New annotation (CR32958) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NR_001675 DDBJ / EMBL / GenBank • AE003667 AJ421756
Other Crossreferences
	miRBase - the home of microRNA data • MI0000116
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 534310 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0046834 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0046834 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0046834 REDfly - Regulatory element database for Drosophila • FBgn0046834
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CR32958   DmiR-1 (Taylor, 2006, Sokol and Ambros, 2005) dmiR-1 (Kwon et al., 2005) miR-1 (Leaman, 2005, Taylor, 2006, Carthew, 2006, Kwon et al., 2005, Brennecke, 2005, Sokol and Ambros, 2005, Nakahara and Carthew, 2004, Boutla et al., 2003, Lai et al., 2003, Grosshans and Slack, 2002, Sempere et al., 2003, Lee et al., 2001, Lagos-Quintana et al., 2001, Stark et al., 2003, Stark et al., 2003, Biemar et al., 2006, Saito et al., 2006, Biemar et al., 2006, Nguyen and Frasch, 2006, Stark et al., 2005, Zeitlinger et al., 2007, Behura, 2007, Okamura et al., 2007) Mir-1 (Robins, 2005) mir-1 (Zeitlinger et al., 2007, Sandmann et al., 2007) Mir1 (Olson, 2006) transfrag 8 (Biemar et al., 2006)
Name Synonym	mir-1
Secondary FlyBase IDs
References ( 34 )
Generate a list of	All references relating to this gene ( 34 )
List References by type	Research paper  ( 17 ) Supplementary material  ( 4 ) Review  ( 9 ) Abstract  ( 3 ) FlyBase analysis  ( 1 )
Recent research papers ( 5 )
	Okamura et al., 2007, Cell 130(1): 89--100 The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. [FBrf0200988] Sandmann et al., 2007, Genes Dev. 21(4): 436--449 A core transcriptional network for early mesoderm development in Drosophila melanogaster. [FBrf0195237] Zeitlinger et al., 2007, Genes Dev. 21(4): 385--390 Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo. [FBrf0195379] Biemar et al., 2006, Proc. Natl. Acad. Sci. USA 103(34): 12763--12768 Comprehensive identification of Drosophila dorsal-ventral patterning genes using a whole-genome tiling array. [FBrf0191950] Saito et al., 2006, Genes Dev. 20(16): 2214--2222 Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome. [FBrf0191820] Show all research papers ...
Recent reviews ( 5 )
	Behura, 2007, Insect Biochem. Molec. Biol. 37(1): 3--9 Insect microRNAs: Structure, function and evolution. [FBrf0194885] Carthew, 2006, Curr. Opin. Genet. Dev. 16(2): 203--208 [title not yet available] [FBrf0191600] Nguyen and Frasch, 2006, Curr. Opin. Genet. Dev. 16(5): 533--539 MicroRNAs in muscle differentiation: lessons from Drosophila and beyond. [FBrf0194438] Olson, 2006, Science 313(5795): 1922--1927 Gene regulatory networks in the evolution and development of the heart. [FBrf0202552] Taylor, 2006, Curr. Biol. 16(1): 20--23 [title not yet available] [FBrf0191607] Show all reviews ...