Gene Dmel\mir-2a-2

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General Information

Symbol

Dmel\mir-2a-2

Species

D. melanogaster

Name

mir-2a-2

Annotation symbol

CR32966

Feature type

miRNA_gene

FlyBase ID

FBgn0046825

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

37F2-37F2

Sequence location

2L:19,569,496..19,569,518 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol mir-2a-2 (CR32966, FBgn0046825). It has the cytological map location 37F2. Its sequence location is 2L:19569496..19569518. Its molecular function is unknown. It is involved in the biological process negative regulation of translational initiation. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

37F2-37F2

Limits computationally determined from genome sequence between P{EP}EP623&P{PZ}spi⁰¹⁰⁶⁸ and P{lacW}l(2)k10239^k10239

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: mir-2b-2? mir-2a-1? mir-2a-2?

(Lagos-Quintana et al., 2001)

Gene Model & Products

Please see the GBrowse view of Dmel\mir-2a-2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

mir-2a-2-RA

FBtr0081275

NR_001667

Additional Transcript Data & Comments

Reported size (kB)

Comments

This miRNA exists in multiple copies in the genome.

(Aravin et al., 2003)

External Data

Crossreferences

miRBase - the home of microRNA data

•

MI0000118

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Name

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

(Sempere et al., 2003)

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0046825

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of mir-2a-2	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mir-2a-2	Class	Mutagen	Stocks	Known lesion
mir-2a-2^Scer\UAS.cLa		in vitro construct \| regulatory fusion	0	Yes
mir-2a-2^{mir2.Scer\UAS.T:Disc\RFP-DsRed2}		in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)E55

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-DsRed2-mir-2}

P{UAS-mir-2.L}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

negative regulation of translational initiation

inferred from direct assay

(Thermann and Hentze, 2007)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

miRNA_gene

(Lagos-Quintana et al., 2001)

nuclear_gene

(Lagos-Quintana et al., 2001)

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

mir-2a-2 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

533477

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\mir-2a-2

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 2 )

cDNA Clones ( 3 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GH27340

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0046825

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

mir-2a-1, mir-2a-2 and mir-2b-2 sequences are clustered within 400bp in 37E3-38A2.

(Sempere et al., 2003)

"mir-2" inhibits translation initiation without affecting mRNA stability.

(Thermann and Hentze, 2007)

Other Comments

New annotation (CR32966) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NR_001667

DDBJ / EMBL / GenBank

•

Other Crossreferences

miRBase - the home of microRNA data

•

MI0000118

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

533477

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0046825

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0046825

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0046825

Synonyms & Secondary IDs ( 10 )

Reported As

Symbol Synonym

CR32966

miR-2

(Carthew, 2006, Landthaler et al., 2004, He and Hannon, 2004, Bartel, 2004, Grosshans and Slack, 2002, Sempere et al., 2003)

mir-2

(Sokol and Ambros, 2003, Lee and Ambros, 2001)

miR2

(Thermann and Hentze, 2007)

miR-2a

(Leaman, 2005, Alvarez-Garcia, 2005, Nakahara and Carthew, 2004, Boutla et al., 2003, Lai, 2003, Stark et al., 2003, Lee et al., 2001, Lagos-Quintana et al., 2001, Stark et al., 2005, Behura, 2007)

miR2a

(Denli et al., 2004, Thermann and Hentze, 2007)

miR2-a

(Gesellchen and Boutros, 2004)

mir-2a

(Sempere et al., 2003)

miR-2a-2

(Lai et al., 2003, Schwarz et al., 2003, Lai, 2002)

mir-2a-2

Name Synonym

mir-2a-2

Secondary FlyBase IDs

References ( 31 )

Generate a list of

All references relating to this gene ( 31 )

List References by type

Recent research papers ( 1 )

Thermann and Hentze, 2007, Nature 447(7146): 875--878: Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation. [FBrf0202080]

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Recent reviews ( 2 )

Behura, 2007, Insect Biochem. Molec. Biol. 37(1): 3--9: Insect microRNAs: Structure, function and evolution. [FBrf0194885]
Carthew, 2006, Curr. Opin. Genet. Dev. 16(2): 203--208: [title not yet available] [FBrf0191600]

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