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Gene Dmel\AGO2

General Information
Symbol	Dmel\AGO2	Species	D. melanogaster
Name	Argonaute 2	Annotation symbol	CG7439
Feature type	protein_coding_gene	FlyBase ID	FBgn0087035
Created / Updated	2007-11-15/2007-11-15
Genomic Location
Chromosome (arm)	3L	Recombination map	3-[42]
Cytogenetic map	71E1-71E1	Sequence location	3L:15,547,217..15,554,146 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Argonaute 2 is referred to in FlyBase by the symbol AGO2 (CG7439, FBgn0087035). It has the cytological map location 71E1. Its sequence location is 3L:15547217..15554146. Its molecular function is described as: protein binding; siRNA binding; translation initiation factor activity; endoribonuclease activity. It is involved in the biological processes described with 14 unique terms, many of which group under: gene silencing by RNA; regulation of biological process; gene silencing; programmed cell death; RNA interference; cell death; cellular localization; pole cell development; gene silencing by miRNA; embryonic pattern specification; dsRNA fragmentation; cellular macromolecule metabolic process; organelle organization and biogenesis; syncytial nuclear migration; response to virus; anatomical structure development. 28 alleles are reported. The phenotypes of these alleles are annotated with: pole cell; condensed chromosome; pole bud; embryonic/first instar larval cuticle. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 71C3-71E5   Left limit from inclusion within Df(3L)BK10 (FBrf0057258) Right limit from inclusion within Df(3L)BK10 (FBrf0057258)
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3-[42]
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\AGO2 for information on other features
Comments on Gene Model
	DGC clone GM07030 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) AGO2-RB FBtr0075559  NM_140518   4050   1214 AGO2-RC FBtr0075560  NM_168626   4059   1217
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein AGO2-PB   FBpp0075312   136.9   1214   10.05     NP_648775   AAF49619 AGO2-PC   FBpp0075313   137.2   1217   10.00     NP_730054   AAF49620
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Argonaute and Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Polynucleotidyl transferase, Ribonuclease H fold (IPR012337) Region of unknown function DUF1785 (IPR014811)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696644   AA697782   AA803111   AA816282   AE003530 AAF49619   AE003530 AAF49620   AI534851   AI945716   AW941283   AY094751 AAM11104   BG639471   BG640026   BI164635   BI165027   BI166132   BI166465   BI169081   BI215715   BI232765   BI238513   BI239989   BI240027   BI242470   BI358052   BI371109   BI373545   BI577439   BI579458   BI580236   BP550128   BP550129   BT003546 AAO39550   CK658543   CL527754   CO155937   CO177453   CO178413   CO184440   CO194968   CO276183   CO279088   CO282449   CO285582   CO301903   CO304377   CO311752   CO313515   CO338396   DQ228766 ABB54719   DQ228767 ABB54720   DQ228768 ABB54721   DQ228769 ABB54722   DQ228770 ABB54723   DQ228771 ABB54724   DQ228772 ABB54725   DQ228773 ABB54726   EC083202   EC086726   EC196356   EC196362   EC202729   EC210943   EC221512   EC223117   EC225307   EC230701   EC231219   EC234082   EC234338   EC235668   EC241670   EC242052   EC244529   EC248565   EC254030   EC263933   EC268008   SD01108
UniProtKB/Swiss-Prot	Q9VUQ5
UniProtKB/TrEMBL	Q2Q3Y0 Q2Q3Y1 Q2Q3Y3 Q2Q3Y4 Q2Q3Y5
Maps to
Does NOT map to
Identified with	bs16a06 (Oliver, 2001.8.16) GM07030 (BDGP Project Members, 2000-, Oliver, 2001.8.16) RE04347 (BDGP Project Members, 2000-, Schneider et al., 2006)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References rescue fragment AGO2[+t10.1] 3L:15,544,282..15,554,436 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator evidence=experimental linked_to=XhoI-FspI_rfrag (Okamura et al., 2004)
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable AGO2414 AGO251B AGO2EY04479 lethal | embryonic stage | maternal effect AGO2dop1 AGO2dop46 semi-lethal | embryonic stage AGO2414 AGO251B Other Phenotypes Allele wild-type AGO2414 reduced cell number AGO2414 reduced cell number | embryonic stage AGO251B cell death increase AGO2dsRNA.cRa mitotic AGO2414 immune response defective AGO2414 Sterility Allele female sterile AGO2dop1 AGO2dop46 fertile AGO2414 AGO2EY04479 Phenotype manifest in Allele nucleus & syncytial blastoderm embryo AGO2414 condensed chromosome AGO2414 pole cell AGO2414 AGO251B pole bud AGO2414 plasma membrane & embryo AGO2dop1 lipid particle & embryo AGO2dop1 embryonic/first instar larval cuticle AGO2dop1
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of AGO2 Class Mutagen Stocks Known lesion AGO2414 loss of function Δ2-3 0 Yes AGO251B Δ2-3 0 Yes AGO2EP3417 P-element activity 1 Yes AGO2EY04479 P-element activity 1 -- AGO2dop1 ethyl methanesulfonate 1 Yes AGO2dop46 Δ2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of AGO2 Class Mutagen Stocks Known lesion AGO2+t10.1 in vitro construct | genomic fragment 0 Yes AGO2Act5C.T:Disc\RFP in vitro construct | coding region fusion in vitro construct | regulatory fusion 0 Yes AGO2C.dsRNA in vitro construct | RNAi 0 Yes AGO2GD17090 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes AGO2MtnA.T:Zzzz\His6 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes AGO2MtnA.T:Zzzz\TAP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes AGO2Scer\UAS.cMa in vitro construct | regulatory fusion 0 Yes AGO2T:Uuuu\T7 in vitro construct | coding region fusion 0 Yes AGO2dsRNA.1-1000 in vitro construct | RNAi 0 Yes AGO2dsRNA.2500-3435 in vitro construct | RNAi 0 Yes AGO2dsRNA.cCa in vitro construct | RNAi 0 Yes AGO2dsRNA.cDa in vitro construct | RNAi 0 Yes AGO2dsRNA.cDb in vitro construct | RNAi 0 Yes AGO2dsRNA.cFa in vitro construct | RNAi 0 Yes AGO2dsRNA.cIa in vitro construct | RNAi 0 Yes AGO2dsRNA.cJa in vitro construct | RNAi 0 Yes AGO2dsRNA.cOa in vitro construct | RNAi 0 Yes AGO2dsRNA.cOb in vitro construct | RNAi 0 Yes AGO2dsRNA.cRa in vitro construct | RNAi 0 Yes AGO2dsRNA.si.1695-1717 in vitro construct | RNAi 0 Yes AGO2dsRNA.si.3293-3315 in vitro construct | RNAi 0 Yes AGO2αTub67C.PM in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)Brd6
Useful duplication	Dp(3;3)M71
Disrupted in	Df(3L)BK10 (Galewsky and Schulz, 1992) Df(3L)Brd6 (Galewsky and Schulz, 1992)
Not disrupted in	Df(3L)Brd15 (Galewsky and Schulz, 1992) Ts(1Lt;3Rt)sc260-15+Ts(2Lt;3Lt)514.2-2-192+Ts(2Rt;3Rt)DTD61 (Galewsky and Schulz, 1992)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{AGO2+t10.1} NA P{tub-AGO2.mat} NA UAS construct P{GD17090} NA P{UAS-AGO2.M} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}AGO2EY04479 NA P{EP}AGO2EP3417 NA P{Mae-UAS.6.11}B2036B2036 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Development of zygotes from homozygous mutant mothers is abnormal from cycle 14 with no separation of yolk mass in the cortical cytoplasm: syncytial nuclei are irregularly spaced and cellularization very variable. Gastrulation is defective and embryos make variable amounts of defective cuticle. (Galewsky and Schulz, 1992)
Gene Ontology: Function, Process & Cellular Component ( 19 )
Molecular Function
CV term Evidence References
endoribonuclease activity inferred from direct assay (Rand et al., 2005)
protein binding inferred from physical interaction with FLYBASE:Fmr1; FB:FBgn0028734 (Ishizuka et al., 2002) inferred from physical interaction with FLYBASE:AGO1; FB:FBgn0026611 (Meyer et al., 2006) inferred from physical interaction with FLYBASE:Dcr-1; FB:FBgn0039016 (Hammond et al., 2001)
siRNA binding inferred from direct assay (Rand et al., 2005) inferred from direct assay (Lingel et al., 2003)
translation initiation factor activity inferred from sequence or structural similarity with UniProt:Q9UL18 (FlyBase, 1992-)
Biological Process
CV term Evidence References
RNA interference traceable author statement (Cerutti, 2003) inferred from direct assay (Matranga et al., 2005) non-traceable author statement (Skipper, 2004) inferred from mutant phenotype (Ishizuka et al., 2002) inferred from direct assay (Rand et al., 2005) inferred from mutant phenotype (Dorner et al., 2006) inferred from mutant phenotype (Rehwinkel et al., 2005) inferred from mutant phenotype (Meyer et al., 2006)
RNA interference, production of siRNA inferred from direct assay (Hammond et al., 2001)
RNA interference, siRNA loading onto RISC inferred from mutant phenotype (Okamura et al., 2004)
RNA interference, targeting of mRNA for destruction traceable author statement (Williams and Rubin, 2002)
autophagic cell death inferred from expression pattern (Gorski et al., 2003)
cellularization inferred from mutant phenotype (Deshpande et al., 2005) inferred from mutant phenotype (Meyer et al., 2006)
defense response to virus inferred from mutant phenotype (Wang et al., 2006)
NOT gene silencing by miRNA inferred from mutant phenotype (Rehwinkel et al., 2005)
lipid particle transport along microtubule inferred from mutant phenotype (Meyer et al., 2006)
pole cell formation inferred from mutant phenotype (Deshpande et al., 2005)
salivary gland cell autophagic cell death inferred from expression pattern (Gorski et al., 2003)
segment polarity determination inferred from genetic interaction with FLYBASE:AGO1; FB:FBgn0026611 (Meyer et al., 2006)
syncytial nuclear migration inferred from mutant phenotype (Deshpande et al., 2005)
translational initiation inferred from sequence or structural similarity with UniProt:Q9UL18 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
RNA-induced silencing complex traceable author statement (Ishizuka et al., 2002) traceable author statement (Nelson et al., 2003) inferred from direct assay (Hammond et al., 2001) traceable author statement (Caudy et al., 2002)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene   gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions AGO2 allele Gene References unspecified AGO1 (Meyer et al., 2006)
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dana\AGO2 (Meyer et al., 2006) Dmoj\AGO2 (Meyer et al., 2006) Dpse\GA20353 (Meyer et al., 2006) Dsan\AGO2 (Obbard et al., 2005.10.3) Dsim\AGO2 (Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3) Dtei\AGO2 (Obbard et al., 2005.10.3) Dvir\AGO2 (Meyer et al., 2006) Dyak\AGO2 (Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3)
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Szeged	- P{EP}AGO2EP3417
VDRC	v49473 w1118; P{GD17090}v49473
Bloomington	16608 y1 w67c23; P{EPgy2}AGO2EY04479 5242 y1 w*; AGO2dop1 red1 e1/TM6B, Tb1
Genomic Clones ( 2 )
	BACR07L07 BACR09C21
cDNA Clones ( 37 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE04347
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE74390 RE36670 RE39801 RE36628 RE34995 RE71115 LP15542 HL03146 RE73489 RE44719 RE10056 RE22310 RE28889 RE60864 LD01139