Gene Dmel\Gr22b

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General Information

Symbol

Dmel\Gr22b

Species

D. melanogaster

Name

Gustatory receptor 22b

Annotation symbol

CG31931

Feature type

protein_coding_gene

FlyBase ID

FBgn0045500

Created / Updated

2005-01-10/2005-01-10

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

22B2-22B2

Sequence location

2L:1,792,057..1,793,268 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Gustatory receptor 22b is referred to in FlyBase by the symbol Gr22b (CG31931, FBgn0045500). It has the cytological map location 22B2. Its sequence location is 2L:1792057..1793268. Its molecular function is unknown. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

22B2-22B2

Limits computationally determined from genome sequence between P{lacW}CG17646^k11021 and P{EP}EP366

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Gr22b for information on other features

Comments on Gene Model

Probable mutation in sequenced strain: premature stop

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Gr22b-RA

FBtr0100188

NM_001014456

1161

386

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Gr22b-PA

FBpp0099546

45.5

386

8.62

NP_001014456

AAX52653

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

AE002940

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0045500

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0045500

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of Gr22b	Class	Mutagen	Stocks	Known lesion
Gr22b¹		spontaneous	2	Yes

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Gr22b	Class	Mutagen	Stocks	Known lesion
Gr22b^GD4884		in vitro construct \| RNAi in vitro construct \| regulatory fusion	4	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)frtz11

Disrupted in

Df(2L)Exel7007

(Ryder, 2004.4.26)

Df(2L)Exel8005

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4884}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 0 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Gr22b allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

72915

Orthologs

Genome-wide drosophilid orthologs

Dyak\GE15401

Curated drosophilid orthologs

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

VDRC

v13001

w¹¹¹⁸ P{GD4884}v13001

v45292

w¹¹¹⁸; P{GD4884}v45292

v45290

w¹¹¹⁸; P{GD4884}v45290/CyO

v45291

w¹¹¹⁸; P{GD4884}v45291/CyO

Tucson

14021-0231.36

y¹ oc^R3.2; Gr22b¹ Gr22d¹ cn¹ CG33964^R4.2 bw¹ sp¹; LysC¹ lab^R4.2 MstProx¹ GstD5¹ Rh6¹ <E>

Bloomington

2057

y¹ oc^R3.2; Gr22b¹ Gr22d¹ cn¹ CG33964^R4.2 bw¹ sp¹; LysC¹ lab^R4.2 MstProx¹ GstD5¹ Rh6¹ <E>

Genomic Clones ( 1 )

BACR25P15

cDNA Clones ( 0 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0045500

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

May be a polymorphic pseudogene in D.melanogaster; in the Oregon R strain the stop codon which is found in the first exon in some strains is a CGA encoding arginine.

(Robertson, 2003.6.24)

Other Comments

Annotation CR31931 renamed CG31931 in release 4.1 of the genome annotation.

(FlyBase Genome Annotators, 2005)

FlyBase curator comment: Stated to be a pseudogene though see FBrf0168007 for explanantion that 'pseudogene' is actually Gr22b¹.

(Dunipace et al., 2001)

New annotation (CR31931) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_001014456

RefSeq (Proteins)

•

NP_001014456

DDBJ / EMBL / GenBank

•

Other Crossreferences

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

72915

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0045500

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0045500

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0045500

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0045500

Synonyms & Secondary IDs ( 7 )

Reported As

Symbol Synonym

CG31931

CR31931

Gr22b

Gr22bP

(Robertson, 2003.6.24)

Gr2940.4

(Scott and Axel, 2001.4.25, )

Name Synonym

Gustatory receptor 22b

(Robertson, 2001.11.11)

Gustatory receptor 2940.4

(Scott and Axel, 2001.4.25)

Secondary FlyBase IDs

FBgn0045491
FBgn0051931

References ( 14 )

Research paper

Takano-Shimizu et al., 2004, Proc. Natl. Acad. Sci. USA 101(39): 14156--14161: Interlocus nonrandom association of polymorphisms in Drosophila chemoreceptor genes. [FBrf0180638]
Thorne et al., 2004, Curr. Biol. 14(12): 1065--1079: Taste perception and coding in Drosophila. [FBrf0179479]
Robertson et al., 2003, Proc. Natl. Acad. Sci. USA 100(Supplement 2): 14537--14542: Molecular evolution of the insect chemoreceptor gene superfamily in Drosophila melanogaster. [FBrf0168007]
Dunipace et al., 2001, Curr. Biol. 11(11): 822--835: Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system. [FBrf0137203]

Review

Amrein and Thorne, 2005, Curr. Biol. 15(17): R673--R684: Gustatory Perception and Behavior in Drosophila melanogaster. [FBrf0187293]
Matsunami and Amrein, 2003, GenomeBiol. 4(7): 220: Taste and pheromone perception in mammals and flies. [FBrf0160781]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Robertson, 2003.6.24, FlyBase error report for CG17868 on Tue Jun 24 18:04:29 2003.: FlyBase error report for CG17868 on Tue Jun 24 18:04:29 2003. [FBrf0161499]
Robertson, 2001.11.11, Annotation revisions.: Annotation revisions. [FBrf0141780]
Scott and Axel, 2001.4.25, Gr genes.: Gr genes. [FBrf0137428]

FlyBase analysis

FlyBase Genome Annotators, 2005, Changes affecting gene model number or type in release 4.1 of the annotated D.melanogaster genome.: Changes affecting gene model number or type in release 4.1 of the annotated D.melanogaster genome. [FBrf0179613]
FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome.: Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]