Gene Dmel\tefu

Help

Close All Open All

General Information

Symbol

Dmel\tefu

Species

D. melanogaster

Name

telomere fusion

Annotation symbol

CG6535

Feature type

protein_coding_gene

FlyBase ID

FBgn0045035

Created / Updated

2003-12-01/2006-05-09

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

88E3-88E4

Sequence location

3R:11,057,922..11,067,884 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene telomere fusion is referred to in FlyBase by the symbol tefu (CG6535, FBgn0045035). It has the cytological map location 88E3-88E4. Its sequence location is 3R:11057922..11067884. Its molecular function is described as: protein kinase activity; phosphotransferase activity, alcohol group as acceptor. It is involved in the biological processes: telomere maintenance; phosphorylation; cell cycle checkpoint; chromatin silencing at telomere; G2/M transition DNA damage checkpoint; telomere capping; chromosome organization and biogenesis. 20 alleles are reported. The phenotypes of these alleles are annotated with 14 unique terms, many of which group under: peripheral nervous system; nervous system; anatomical structure; imaginal precursor; organ system; intracellular non-membrane-bounded organelle; adult; intracellular organelle part; cell part; intracellular organelle. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88E3-88E4

Limits computationally determined from genome sequence between P{EP}CG33967^EP666 and P{lacW}Hsc70-4^L3929

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

88B5-88C4

(Queiroz-Machado et al., 2001)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: tefu- Hsc70-4+

(Hing et al., 1999)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: tefu? Hsc70-4+ CG6499+

(Bronk et al., 2001)

Gene Model & Products

Please see the GBrowse view of Dmel\tefu for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

tefu-RB

FBtr0110874

NM_001043247

8412

2767

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

tefu-PB

FBpp0110174

318.0

2767

7.37

NP_001036712

ABI31168

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Phosphatidylinositol 3- and 4-kinase, catalytic (IPR000403)

PIK-related kinase, FATC (IPR003152)

Protein kinase-like (IPR011009)

PIK-related kinase (IPR014009)

Ataxia-Telangiectasia Mutated (IPR015519)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q5EAK6

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

AT01448

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

insertion site

tefu[1]
3R:11,063,165..11,063,165

comment=Mutation is an insertion of 4bp which changes C969 to a stop.
evidence=experimental

(Oikemus et al., 2004)

point mutation

tefu[atm-6]
3R:11,063,141..11,063,142

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
pr_change=W977@|tefu-PA
reported_pr_change=W977@

(Silva et al., 2004)

point mutation

tefu[red31]
3R:11,062,729..11,062,729

comment=Difference between reported and FlyBase inferred amino acid residue number is due to the authors describing a protein of 2768aa versus 2429 in FlyBase. Position of mutation on reference sequence inferred by FlyBase curator based on author statement (Figure 1).
evidence=experimental
na_change=C11062729A
pr_change=S1434@|tefu-PB
reported_na_change=C4320A
reported_pr_change=S1434@

(Oikemus et al., 2004)

rescue fragment

tefu[+t14.5]
3R:11,056,166..11,070,718

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=PvuI-ApaI_rfrag

(Silva et al., 2004)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

9524

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0045035

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

tefu^red31

lethal | recessive

tefu¹

lethal | recessive | conditional - heat sensitive

tefu^atm-8

lethal | conditional - heat sensitive (with Df(3R)PG4)

tefu^atm-8

lethal | pupal stage | recessive

Other Phenotypes

Allele

visible | recessive | conditional - heat sensitive

tefu^atm-8

locomotor behavior defective | male | conditional - heat sensitive (with Df(3R)PG4)

tefu^atm-8

locomotor behavior defective | female | conditional - heat sensitive (with Df(3R)PG4)

tefu^atm-8

cell death increase

tefu^Δ356

cell death increase | conditional - heat sensitive

tefu^atm-8

mitotic cell cycle defective

mitotic cell cycle defective (with tefu^atm-6)

tefu^atm-3

mitotic cell cycle defective (with tefu^atm-3)

tefu^atm-6

radiation sensitive

radiation sensitive (with Df(3R)PG4)

tefu^atm-8

Sterility

Allele

female sterile | recessive

Phenotype manifest in

Allele

eye (with Df(3R)PG4)

tefu^Δ356

wing (with Df(3R)PG4)

tefu^Δ356

macrochaeta (with Df(3R)PG4)

tefu^Δ356

eye (with tefu¹)

tefu^Δ356

wing (with tefu¹)

tefu^Δ356

macrochaeta (with tefu¹)

tefu^Δ356

eye

wing

macrochaeta

tefu^Δ356

dorsal mesothoracic disc

tefu^Δ356

eye disc

tefu^Δ356

mitosis & nuclear chromosome

chromosome, telomeric region

eye (with tefu^Δ356)

tefu¹

wing (with tefu^Δ356)

tefu¹

macrochaeta (with tefu^Δ356)

tefu¹

antenna & pupa

wing & pupa

eye & pupa

leg & pupa

chaeta & pupa

metaphase & condensed nuclear chromosome (with tefu^atm-6)

tefu^atm-3

anaphase & condensed nuclear chromosome (with tefu^atm-6)

tefu^atm-3

chromosome, telomeric region (with tefu^atm-6)

tefu^atm-3

cleavage nucleus | germ-line clone | maternal effect

tefu^atm-6

spindle | germ-line clone | maternal effect

tefu^atm-6

chorion | germ-line clone | maternal effect

tefu^atm-6

dorsal appendage | germ-line clone | maternal effect

tefu^atm-6

mitosis & nuclear chromosome | maternal effect | germ-line clone

tefu^atm-6

condensed chromosome

tefu^atm-6

metaphase & condensed nuclear chromosome (with tefu^atm-3)

tefu^atm-6

anaphase & condensed nuclear chromosome (with tefu^atm-3)

tefu^atm-6

chromosome, telomeric region (with tefu^atm-3)

tefu^atm-6

wing | conditional - heat sensitive (with Df(3R)PG4)

tefu^atm-8

eye | conditional - heat sensitive (with Df(3R)PG4)

tefu^atm-8

adult thorax & chaeta | conditional ts (with Df(3R)PG4)

tefu^atm-8

eye | conditional - heat sensitive

tefu^atm-8

wing | conditional - heat sensitive

tefu^atm-8

eye-antennal disc | conditional - heat sensitive

tefu^atm-8

eye disc | anterior | conditional - heat sensitive

tefu^atm-8

ommatidium

tefu^unspecified

interommatidial bristle

tefu^unspecified

anaphase & condensed nuclear chromosome

tefu^unspecified

Classical Alleles ( 17 )

For All Classical Alleles Show

Allele of tefu	Class	Mutagen	Stocks	Known lesion
tefu¹	hypomorph		0	Yes
tefu^GS13617			0	Yes
tefu^atm-1		ethyl methanesulfonate	0	--
tefu^atm-2		ethyl methanesulfonate	0	--
tefu^atm-3		ethyl methanesulfonate	1	--
tefu^atm-4		ethyl methanesulfonate	0	--
tefu^atm-5		ethyl methanesulfonate	0	--
tefu^atm-6	loss of function	ethyl methanesulfonate	1	Yes
tefu^atm-7		ethyl methanesulfonate	0	--
tefu^atm-8	hypomorph	ethyl methanesulfonate	1	--
tefu^e03093		piggyBac transposase	1	--
tefu^red31			0	--
tefu^stg		recombination	0	Yes
tefu^unspecified			0	--
tefu^wk		recombination	0	Yes
tefu^Δ11	hypomorph		0	Yes
tefu^Δ356	loss of function, hypomorph		0	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of tefu	Mutagen	Stocks	Known lesion
tefu^+t14.5	in vitro construct \| genomic fragment	0	Yes
tefu^GD11950	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
tefu^Scer\UAS.cOa	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)red3l

Useful duplication

Dp(3;3)C123.3

Disrupted in

Df(3R)BSC471

(Christensen et al., 2008.4.15)

Df(3R)Exel7323

(Ryder, 2004.4.26)

Df(3R)Hsc70-4^Δ11

(Bronk et al., 2001)

Df(3R)Hsc70-4^Δ356

(Bronk et al., 2001, Bi et al., 2004)

Df(3R)PG4

(Silva et al., 2004)

Df(3R)red3l

(Queiroz-Machado et al., 2001)

Not disrupted in

Df(3R)293γ5

(Queiroz-Machado et al., 2001)

Df(3R)red-P52

(Queiroz-Machado et al., 2001)

Df(3R)su(Hw)7

(Queiroz-Machado et al., 2001)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11950}

P{UAS-atm.O}

characterization construct

P{tefu^+t14.5}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}tefu^e03093

insertion of mobile activating element

P{GSV1}EP-J76^EP-J76

P{GSV6}tefu^GS13617

Related Comments

Please look at the allele reports for the complete phenotype data

tefu mutants show a high level of spontaneous telomere fusions and other chromosome abnormalities in larval neuroblasts.

(Silva et al., 2004)

tefu mutants show a high level of spontaneous telomere fusions in larval neuroblasts.

(Bi et al., 2004)

tefu mutants show a high level of spontaneous telomere fusions in larval neuroblasts. Mutants show an increase in spontaneous p53-dependent apoptosis.

(Song et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

phosphotransferase activity, alcohol group as acceptor

inferred from electronic annotation with InterPro:IPR000403

(FlyBase Curators et al., 2004-)

protein kinase activity

non-traceable author statement

(Morrison et al., 2000)

inferred from sequence or structural similarity

(Sekelsky et al., 2000)

Biological Process

CV term

Evidence

References

G2/M transition DNA damage checkpoint