Gene Dmel\let-7

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General Information

Symbol

Dmel\let-7

Species

D. melanogaster

Name

let-7

Annotation symbol

CR32968

Feature type

miRNA_gene

FlyBase ID

FBgn0043885

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

36F5-36F5

Sequence location

2L:18,472,044..18,472,067 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol let-7 (CR32968, FBgn0043885). It has the cytological map location 36F5. Its sequence location is 2L:18472044..18472067. Its molecular function is unknown. It is involved in the biological processes: regulation of development, heterochronic; metamorphosis. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36F5-36F5

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: mir-100+ let-7+ mir-125+

(Sokol, 2003.2.18)

Gene Model & Products

Please see the GBrowse view of Dmel\let-7 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

let-7-RA

FBtr0081065

NR_001664

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

miRBase - the home of microRNA data

•

MI0000416

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Name

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

prepupal stage-adult stage

(Sempere et al., 2003)

pupal stage | 0-18 h

(Bashirullah et al., 2003)

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0043885

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of let-7	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of let-7	Class	Mutagen	Stocks	Known lesion
let-7^MtnA.PR		in vitro construct \| regulatory fusion	0	Yes
let-7^cRa		in vitro construct \| other	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)M36F-S5

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 2 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

metamorphosis

inferred from expression pattern

(Sempere et al., 2002)

regulation of development, heterochronic

traceable author statement

(Banerjee and Slack, 2002)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

miRNA_gene

(Lagos-Quintana et al., 2001)

nuclear_gene

(Lagos-Quintana et al., 2001)

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

let-7 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

534090

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\let-7

(Lai et al., 2003)

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 2 )

cDNA Clones ( 0 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0043885

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Hormone induced expression of let-7 may control developmental stage transitions.

(Sempere et al., 2002)

Comments About Molecular Function

A developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 small temporal RNA (stRNA).

(Hutvagner et al., 2001)

let-7 and mir-125 appear to be expressed from a common precursor RNA.

(Bashirullah et al., 2003)

mir-100, mir-125 and let-7 sequences are clustered within 800bp in 36E5--36F2.

(Sempere et al., 2003)

A long primary transcript comprising all the hairpin precursor sequences for mir-100, let-7 and mir-125 has been detected by RT-PCR. Overall orientation not stated: mir-100+ let-7+ mir-125+

(Aravin et al., 2003)

Other Comments

New annotation (CR32968) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NR_001664

DDBJ / EMBL / GenBank

•

AE003659

Other Crossreferences

miRBase - the home of microRNA data

•

MI0000416

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

534090

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0043885

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0043885

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0043885

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/dbzhnsky/let7-1.htm

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

CR32968

Let-7

(Jing et al., 2005)

let-7

(Caygill and Johnston, 2007, Stark et al., 2005, Ruby et al., 2007, Behura, 2007, Ruby et al., 2007, FÃ¶rstemann et al., 2007, Tomari et al., 2007, Caygill and Johnston, 2008, O'Farrell et al., 2008)

Name Synonym

let-7

Secondary FlyBase IDs

References ( 54 )

Generate a list of

All references relating to this gene ( 54 )

List References by type

Recent research papers ( 4 )

O'Farrell et al., 2008, Dev. Dynamics 237(1): 196--208: Regulation of the Drosophila lin-41 homologue dappled by let-7 reveals conservation of a regulatory mechanism within the LIN-41 subclade. [FBrf0201979]
FÃ¶rstemann et al., 2007, Cell 130(2): 287--297: Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by Dicer-1. [FBrf0200521]
Ruby et al., 2007, Nature 448(7149): 83--86: Intronic microRNA precursors that bypass Drosha processing. [FBrf0201222]
Tomari et al., 2007, Cell 130(2): 299--308: Sorting of Drosophila small silencing RNAs. [FBrf0201844]

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Recent reviews ( 1 )

Behura, 2007, Insect Biochem. Molec. Biol. 37(1): 3--9: Insect microRNAs: Structure, function and evolution. [FBrf0194885]

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