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Gene Dmel\v(2)k05816

General Information
Symbol	Dmel\v(2)k05816	Species	D. melanogaster
Name	v(2)k05816	Annotation symbol	CG3524
Feature type	protein_coding_gene	FlyBase ID	FBgn0042627
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	23C5-23C5	Sequence location	2L:3,043,973..3,055,601 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol v(2)k05816 (CG3524, FBgn0042627). It has the cytological map location 23C5. Its sequence location is 2L:3043973..3055601. Its molecular function is described by 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity; [acyl-carrier-protein] S-malonyltransferase activity; hydrolase activity, acting on ester bonds; oxidoreductase activity; cofactor binding. It is involved in the biological process biosynthetic process. 9 alleles are reported. The phenotypes of these alleles are annotated with: wing; leg. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 23C5-23C5   Limits computationally determined from genome sequence between P{PZ}lilli00632 and P{PZ}toc01361
Experimentally Determined Cytological Location
Cytogenetic map Notes References 23D1-23D2 (determined by in situ hybridisation)   (BDGP Project Members, 2000-) 23D1-23D2   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\v(2)k05816 for information on other features
Comments on Gene Model
	DGC clone appears problematic (GH02912): incomplete CDS.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) v(2)k05816-RA FBtr0077657  NM_139356   7590   2409
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein v(2)k05816-PA   FBpp0077342   265.9   2409   7.08     NP_647613   AAF51149
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0042627
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Beta-ketoacyl synthase (IPR000794) Thioesterase (IPR001031) Acyl transferase region (IPR001227) Short-chain dehydrogenase/reductase SDR (IPR002198) Phosphopantetheine-binding (IPR006163) Acyl carrier protein-like (IPR009081) GroES-like (IPR011032) Alcohol dehydrogenase, zinc-binding (IPR013149) Beta-ketoacyl synthase, N-terminal (IPR014030) Beta-ketoacyl synthase, C-terminal (IPR014031) Acyl transferase (IPR014043) NAD(P)-binding (IPR016040) Acyl transferase/acyl hydrolase/lysophospholipase (IPR016035) Malonyl-CoA ACP transacylase, ACP-binding (IPR016036) Thiolase-like, subgroup (IPR016038) Thiolase-like (IPR016039)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003581 AAF51149   AI064682   AI107683   AI293020   AI532314   AQ025771   AQ026476   AW940113   AY122087 AAM52599   BF496610   BG633210   CK132381   CZ471277   CZ471278   CZ473761   CZ473762   CZ478023   CZ478024   CZ485306   CZ485307   EC244468
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MR70 Q9VQL6
Maps to
Does NOT map to
Identified with	GH02912 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 5517
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI402921 AI532314 AI064682
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0042627 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0042627 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0042627
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele visible (with Df(2L)JS17) v(2)k05816k05816 Phenotype manifest in Allele wing (with Df(2L)JS17) v(2)k05816k05816 leg (with Df(2L)JS17) v(2)k05816k05816
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of v(2)k05816 Class Mutagen Stocks Known lesion v(2)k05816EP695 P-element activity 2 -- v(2)k05816EY01808 P-element activity 1 -- v(2)k05816c07138 piggyBac transposase 1 -- v(2)k05816d04154 P-element activity 1 -- v(2)k05816e00126 piggyBac transposase 1 -- v(2)k05816f02757 piggyBac transposase 1 -- v(2)k05816f02758 piggyBac transposase 0 -- v(2)k05816k05816 P-element activity 2 Yes
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of v(2)k05816 Class Mutagen Stocks Known lesion v(2)k05816GD1555 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)JS17
Disrupted in	Df(2L)Exel7014 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1555} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}v(2)k05816c07138 NA PBac{RB}v(2)k05816e00126 NA PBac{WH}v(2)k05816f02757 NA PBac{WH}v(2)k05816f02758 NA P{UASp-YFP.Rab10.T23N}15 NA insertion of enhancer trap P{lacW}v(2)k05816k05816 No insertion of mobile activating element P{EPgy2}v(2)k05816EY01808 NA P{EP}EP600 NA P{EP}v(2)k05816EP695 NA P{XP}v(2)k05816d04154 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 8 )
Molecular Function
CV term Evidence References
3-oxoacyl-[acyl-carrier-protein] synthase activity inferred from sequence or structural similarity with UniProtKB:P12785 (FlyBase, 1992-)
[acyl-carrier-protein] S-acetyltransferase activity inferred from sequence or structural similarity with UniProtKB:P12785 (FlyBase, 1992-)
[acyl-carrier-protein] S-malonyltransferase activity inferred from sequence or structural similarity with UniProtKB:P12785 (FlyBase, 1992-)
cofactor binding inferred from electronic annotation with InterPro:IPR006163 (FlyBase Curators et al., 2004-)
hydrolase activity, acting on ester bonds inferred from electronic annotation with InterPro:IPR001031 (FlyBase Curators et al., 2004-)
oxidoreductase activity inferred from electronic annotation with InterPro:IPR002198 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
biosynthetic process inferred from electronic annotation with InterPro:IPR000794, InterPro:IPR001031 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
lipid particle inferred from direct assay (Beller et al., 2006) inferred from direct assay (Beller et al., 2006)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions v(2)k05816 allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 72883 Drosophila PIMRider - The Drosophila Protein Interaction map • 522089
Orthologs
Genome-wide drosophilid orthologs	Dere\GG24919 Dgri\GH10962 Dpse\GA17498 Dyak\GE18209
Curated drosophilid orthologs	Dpse\GA17498
Linkouts	InParanoid orthologs - Eukaryotic orthologs • MGI:95485 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Szeged	- P{EP}EP600 - P{EP}v(2)k05816EP695
Harvard	- P{XP}v(2)k05816d04154 - PBac{PB}v(2)k05816c07138 - PBac{RB}v(2)k05816e00126 - PBac{WH}v(2)k05816f02757
VDRC	v4290 w1118; P{GD1555}v4290 v4291 w1118; P{GD1555}v4291
Bloomington	9788 y1 w*; P{UASp-YFP.Rab10.T23N}15 17192 w1118; P{EP}v(2)k05816EP695/CyO 15538 y1 w67c23; P{EPgy2}v(2)k05816EY01808
Kyoto	102421 Df(1)w67c23, y1 ; P{lacW}v(2)k05816k05816 / CyO
	More stocks available...
Genomic Clones ( 2 )
	BACR48B06 BACR23N06
cDNA Clones ( 6 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP13874 AT10488 GH16082 GH05495 GH22337 SD03777
Other clones
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0042627 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0042627
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139356 RefSeq (Proteins) • NP_647613 DDBJ / EMBL / GenBank • AE003581 AAF51149 AI064682 AQ025771 AQ026476 AW940113 AY122087 AAM52599 CZ471277 CZ471278 CZ473761 CZ473762 CZ478023 CZ478024 CZ485306 CZ485307 UniProtKB/TrEMBL • Q8MR70 Q9VQL6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Beta-ketoacyl synthase (IPR000794) Thioesterase (IPR001031) Acyl transferase region (IPR001227) Short-chain dehydrogenase/reductase SDR (IPR002198) Phosphopantetheine-binding (IPR006163) Acyl carrier protein-like (IPR009081) GroES-like (IPR011032) Alcohol dehydrogenase, zinc-binding (IPR013149) Beta-ketoacyl synthase, N-terminal (IPR014030) Beta-ketoacyl synthase, C-terminal (IPR014031) Acyl transferase (IPR014043) NAD(P)-binding (IPR016040) Acyl transferase/acyl hydrolase/lysophospholipase (IPR016035) Malonyl-CoA ACP transacylase, ACP-binding (IPR016036) Thiolase-like, subgroup (IPR016038) Thiolase-like (IPR016039)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 72883 DEDB - Drosophila exon database: splicing graphs • 5517 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0042627 Drosophila PIMRider - The Drosophila Protein Interaction map • 522089 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0042627 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0042627 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0042627 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0042627 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0042627 InParanoid orthologs - Eukaryotic orthologs • MGI:95485 Mus musculus (Informatics) PANTHER - Protein classification by function, families, and pathways • FBgn0042627
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG3524 (Beller et al., 2006) DM_7295849 (Nordling et al., 2002) v(2)k05816 (Beller et al., 2006, Dionne et al., 2006, Zhang et al., 2007)
Name Synonym	v(2)k05816
Secondary FlyBase IDs
	FBgn0031502
References ( 15 )
Research paper	Beller et al., 2006, Mol. Cell. Proteomics 5(6): 1082--1094 Characterization of the Drosophila lipid droplet subproteome. [FBrf0192399] Dionne et al., 2006, Curr. Biol. 16(20): 1977--1985 Akt and foxo dysregulation contribute to infection-induced wasting in Drosophila. [FBrf0195260] Nordling et al., 2002, Europ. J. Biochem. 269(17): 4267--4276 Medium-chain dehydrogenases/reductases (MDR). Family characterizations including genome comparisons and active site modelling. [FBrf0151967]
Supplementary material	Zhang et al., 2007, Genetics 176(2): Supplement to Zhang et al., 2007. [FBrf0203184] Beller et al., 2006, Mol. Cell. Proteomics 5(6): Supplemental data for characterization of the Drosophila lipid droplet subproteome. [FBrf0200342]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335] Levis, 2001.11.16, v(2)k05816 has a P insertion in CG3524. v(2)k05816 has a P insertion in CG3524. [FBrf0141845]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]