Gene Dmel\CG3253

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General Information

Symbol

Dmel\CG3253

Species

D. melanogaster

Name

CG3253

Annotation symbol

CG3253

Feature type

protein_coding_gene

FlyBase ID

FBgn0041706

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

60B10-60B11

Sequence location

2R:20,002,190..20,003,887 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG3253 (FBgn0041706). It has the cytological map location 60B10-60B11. Its sequence location is 2R:20002190..20003887. Its molecular function is described as N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity. The biological processes in which it is involved are not known. 5 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60B10-60B11

Limits computationally determined from genome sequence between P{EP}EP503 and P{lacW}Nop60B^k05318

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60C-60C

(determined by in situ hybridisation)

(Nouaud et al., 1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: CG3253- CG4049+

(Nouaud et al., 1999)

Gene Model & Products

Please see the GBrowse view of Dmel\CG3253 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG3253-RA

FBtr0072251

NM_138042

1524

389

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG3253-PA

FBpp0072160

45.3

389

10.07

NP_611886

AAF47166

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0041706

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W1A7

Maps to

Does NOT map to

Identified with

GH04269

(Nouaud et al., 1999, BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

1671

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI064022

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0041706

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0041706

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of CG3253	Mutagen	Stocks	Known lesion
CG3253^e00156	piggyBac transposase	1	--
CG3253^f01308	piggyBac transposase	1	--
CG3253^f01495	piggyBac transposase	1	--
CG3253^f06846	piggyBac transposase	0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG3253	Class	Mutagen	Stocks	Known lesion
CG3253^GD10917		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)vw

Disrupted in

Df(2R)Exel6081

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10917}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}CG3253^e00156

PBac{WH}CG3253^f01308

PBac{WH}CG3253^f01495

PBac{WH}CG3253^f06846

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity

inferred from sequence or structural similarity with EMBL:AF029893

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG3253 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63440

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521717

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11373

Dere\GG22943

Dgri\GH20168

Dmoj\GI18863

Dper\GL11668

Dpse\GA16964

Dsec\GM18308

Dsim\GD15463

Dvir\GJ20432

Dwil\GK21937

Dyak\GE14380

Curated drosophilid orthologs

Dana\BEST:GH04269

(Nouaud et al., 1999)

Dboc\BEST:GH04269

(Nouaud et al., 1999)

Ddav\BEST:GH04269

(Nouaud et al., 1999)

Dmim\BEST:GH04269

(Nouaud et al., 1999)

Dnik\BEST:GH04269

(Nouaud et al., 1999)

Dpse\GA16964

Dtsa\BEST:GH04269

(Nouaud et al., 1999)

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000000834
Anopheles gambiae (Ensembl)
ENSBTAP00000030011
Bos taurus (Ensembl)
ENSCAFP00000018798
Canis familiaris (Ensembl)
ENSCINP00000011597
Ciona intestinalis (Ensembl)
NEWSINFRUP00000135217
Takifugu rubripes (Ensembl)
ENSGALP00000020480
Gallus gallus (Ensembl)
ENSP00000309096
Homo sapiens (Ensembl)
ENSMMUP00000021033
Macaca mulatta (Ensembl)
ENSMODP00000010380
Monodelphis domestica (Ensembl)
ENSPTRP00000006755
Pan troglodytes (Ensembl)
ENSRNOP00000027233
Rattus norvegicus (Ensembl)
GSTENP00005647001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000044443
Xenopus tropicalis (Ensembl)
WBGene00027757
Caenorhabditis briggsae (Wormbase)
WBGene00010716
Caenorhabditis elegans (Wormbase)
DDB0231851
Dictyostelium discoideum (Dictybase)
MGI:1919680
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Harvard

PBac{RB}CG3253^e00156

PBac{WH}CG3253^f01308

PBac{WH}CG3253^f01495

VDRC

v26159

w¹¹¹⁸ P{GD10917}v26159

Genomic Clones ( 1 )

BACR27M17

cDNA Clones ( 10 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH04269

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0041706

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0041706

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG3253 BEST:GH04269 Source for merge of CG3253 BEST:GH04269 was a shared cDNA (GH04269) (date:011030).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_138042

RefSeq (Proteins)

•

NP_611886

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q9W1A7

Other Crossreferences

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63440

DEDB - Drosophila exon database: splicing graphs

•

1671

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0041706

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521717

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0041706

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0041706

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0041706

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0041706

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000000834
Anopheles gambiae (Ensembl)
ENSBTAP00000030011
Bos taurus (Ensembl)
ENSCAFP00000018798
Canis familiaris (Ensembl)
ENSCINP00000011597
Ciona intestinalis (Ensembl)
NEWSINFRUP00000135217
Takifugu rubripes (Ensembl)
ENSGALP00000020480
Gallus gallus (Ensembl)
ENSP00000309096
Homo sapiens (Ensembl)
ENSMMUP00000021033
Macaca mulatta (Ensembl)
ENSMODP00000010380
Monodelphis domestica (Ensembl)
ENSPTRP00000006755
Pan troglodytes (Ensembl)
ENSRNOP00000027233
Rattus norvegicus (Ensembl)
GSTENP00005647001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000044443
Xenopus tropicalis (Ensembl)
WBGene00027757
Caenorhabditis briggsae (Wormbase)
WBGene00010716
Caenorhabditis elegans (Wormbase)
DDB0231851
Dictyostelium discoideum (Dictybase)
MGI:1919680
Mus musculus (Informatics)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0041706

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

BEST:GH04269

CG3253

Name Synonym

CG3253

L-gene

(Nouaud et al., 1999)

Secondary FlyBase IDs

FBgn0034977

References ( 11 )

Research paper

Inlow and Restifo, 2004, Genetics 166(2): 835--881: Molecular and comparative genetics of mental retardation. [FBrf0174714]
Nouaud et al., 1999, Molec. Biol. Evol. 16(11): 1503--1510: A P element has induced intron formation in Drosophila. [FBrf0111992]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Levis, 2001.7.6, MdaPK gene may = gek gene (?).: MdaPK gene may = gek gene (?). [FBrf0138604]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.: Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Curated genome annotation

Heiman, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126693]