Gene Dmel\Rheb

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General Information

Symbol

Dmel\Rheb

Species

D. melanogaster

Name

Rheb

Annotation symbol

CG1081

Feature type

protein_coding_gene

FlyBase ID

FBgn0041191

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

83B2-83B2

Sequence location

3R:1,394,618..1,396,213 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Rheb (CG1081, FBgn0041191). It has the cytological map location 83B2. Its sequence location is 3R:1394618..1396213. Its molecular function is described as: GTPase activity; GTP binding. It is involved in the biological processes: imaginal disc growth; response to starvation; G1/S transition of mitotic cell cycle; cell growth; positive regulation of cell growth; small GTPase mediated signal transduction. 30 alleles are reported. The phenotypes of these alleles are annotated with 17 unique terms, many of which group under: organ system; anatomical structure; peripheral nervous system; nervous system; cell cycle; adult; adult segment; cell cycle process; adult mesothoracic segment; imaginal precursor. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83B2-83B2

Limits computationally determined from genome sequence between P{PZ}Snr1⁰¹³¹⁹&P{PZ}Itp-r83A⁰⁵⁶¹⁶ and P{lacW}Atu^s1938

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Rheb for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Rheb-RA

FBtr0078693

NM_169068

1250

182

Rheb-RB

FBtr0078694

NM_169069

1021

182

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Rheb-PA

FBpp0078342

20.7

182

5.32

NP_730950

AAF52004

Rheb-PB

FBpp0078343

20.7

182

5.32

NP_730951

AAF52005

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0041191

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ras GTPase (IPR001806)

Ras small GTPase, Ras type (IPR003577)

Small GTP-binding protein (IPR005225)

Ras (IPR013753)

Ras small GTPase, Rheb-related (IPR015602)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VND8

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

GH10361

(BDGP Project Members, 2000-, Saucedo, 2002.10.25)

GH14625

(Urano et al., 2000)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

complex substitution

Rheb[2G5]
3R:1,395,193..1,395,296

comment=A combination of a deletion of 11 nucleotides and an insertion of six nucleotides in the second intron, generating a putative novel splice acceptor site.
evidence=experimental

(Stocker et al., 2003)

point mutation

Rheb[3M2]
3R:1,394,946..1,394,946

comment=Mutation is in translation initiation codon.
evidence=experimental

(Stocker et al., 2003)

point mutation

Rheb[1TI]
3R:1,394,994..1,394,994

comment=A splice donor mutation (GT to AT) predicted to result in a protein consisting of the amino-terminal 16 amino acids followed by DEFSQKVPPEALTLLLLALVIA
evidence=experimental
na_change=G1394994A

(Stocker et al., 2003)

point mutation

Rheb[501]
3R:1,395,379..1,395,379

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=A1395379G
pr_change=I68M|Rheb-PB,I68M|Rheb-PA
reported_pr_change=I68M

(Stocker et al., 2003)

point mutation

Rheb[7A1]
3R:1,395,386..1,395,388

comment=FlyBase curator could not determine nucleotide substitution based on amino acid change reported by author.
evidence=experimental
reported_pr_change=V71K

(Stocker et al., 2003)

point mutation

Rheb[4L1]
3R:1,395,476..1,395,476

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=A1395476T
pr_change=K101|Rheb-PB,K101|Rheb-PA
reported_pr_change=K101@

(Stocker et al., 2003)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

6310

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI113871

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0041191

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0041191

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0041191

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable (with Rheb^7A1)

Rheb^44.1

viable (with Rheb^44.1)

viable (with Rheb^3M2)

Rheb^44.1

viable (with Rheb^2G5)

Rheb^44.1

lethal | recessive

Rheb^LA01053

lethal | recessive | second instar larval stage, with Scer\GAL4[-]:

Rheb^AV4

lethal | larval stage (with Rheb^PΔ2)

Rheb^PΔ1

lethal | larval stage (with Rheb^PΔ1)

Rheb^PΔ2

lethal | larval stage | recessive

Rheb^AV4

lethal | pupal stage (with Rheb^7A1)

Rheb^26.2

lethal | pupal stage (with Rheb^3M2)

Rheb^26.2

lethal | pupal stage (with Rheb^26.2)

lethal, with Scer\GAL4^byn-Gal4

Rheb^AV4

semi-lethal | larval stage (with Rheb^7A1)

Rheb^26.2

semi-lethal | larval stage (with Rheb^26.2)

Rheb^7A1

Other Phenotypes

Allele

visible, with Scer\GAL4^ptc-559.1

Rheb^Scer\UAS.cSa

small body | larval stage (with Rheb^7A1)

Rheb^26.2

small body | larval stage (with Rheb^26.2)

Rheb^7A1

small body | first instar larval stage | recessive, with Scer\GAL4[-]:

Rheb^AV4

small body | pupal stage (with Rheb^7A1)

Rheb^26.2

small body | pupal stage (with Rheb^26.2)

Rheb^7A1

small body | adult stage (with Rheb^7A1)

Rheb^44.1

small body | adult stage (with Rheb^44.1)

small body | adult stage (with Rheb^3M2)

Rheb^44.1

small body | adult stage (with Rheb^2G5)

Rheb^44.1

reduced cell number | somatic clone

Rheb^2D1

reduced cell size | somatic clone

large body, with Scer\GAL4^GMR.PF

Rheb^{EP50.084-loxP}

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

Rheb^Scer\UAS.cSa

increased cell size, with Scer\GAL4^en-e16E

Rheb^Scer\UAS.cSa

increased cell size, with Scer\GAL4^GMR.PF

Rheb^Scer\UAS.cSa

eclosion rhythm defective | dominant | drug conditional, with Scer\GAL4[-]:

Rheb^AV4

cell size defective

cell size defective | somatic clone, with Scer\GAL4[-]:

Rheb^AV4

cell size defective, with Scer\GAL4^ap-md544

Rheb^Scer\UAS.cPa

mitotic cell cycle defective, with Scer\GAL4^Act5C.PP

Rheb^Scer\UAS.cSa

developmental rate defective (with Rheb^PΔ2)

Rheb^PΔ1

developmental rate defective (with Rheb^PΔ1)

Rheb^PΔ2

developmental rate defective, with Scer\GAL4[-]:

Rheb^AV4

Sterility

Allele

female sterile (with Rheb^7A1)

Rheb^44.1

female sterile (with Rheb^44.1)

Rheb^7A1

Phenotype manifest in

Allele

imaginal disc (with Rheb^3M2)

Rheb^26.2

larval salivary gland (with Rheb^3M2)

Rheb^26.2

ommatidium | somatic clone

eye | somatic clone

ommatidium (with Rheb^44.1)

wing (with Rheb^44.1)

imaginal disc (with Rheb^26.2)

Rheb^3M2

larval salivary gland (with Rheb^26.2)

Rheb^3M2

ommatidium (with Rheb^3M2)

Rheb^44.1

wing (with Rheb^3M2)

Rheb^44.1

ommatidium (with Rheb^7A1)

Rheb^44.1

wing (with Rheb^7A1)

Rheb^44.1

ovary (with Rheb^7A1)

Rheb^44.1

ommatidium (with Rheb^2G5)

Rheb^44.1

ovary (with Rheb^44.1)

Rheb^7A1

larval hindgut, with Scer\GAL4^byn-Gal4

Rheb^AV4

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^Act5C.PI

Rheb^AV4

head, with Scer\GAL4^Act5C.PI

Rheb^AV4

ommatidium, with Scer\GAL4^Act5C.PI

Rheb^AV4

interommatidial bristle | supernumerary, with Scer\GAL4^Act5C.PI

Rheb^AV4

antenna, with Scer\GAL4^Act5C.PI

Rheb^AV4

head | somatic clone, with Scer\GAL4[-]:

Rheb^AV4

eye | somatic clone, with Scer\GAL4[-]:

Rheb^AV4

ommatidium | somatic clone, with Scer\GAL4[-]:

Rheb^AV4

S phase

Rheb^{Act5C.T:Zzzz\FLAG}

ommatidium, with Scer\GAL4^GMR.PF

Rheb^EP50.084

wing | dorsal compartment, with Scer\GAL4^ap-md544

Rheb^Scer\UAS.cPa

wing | posterior compartment, with Scer\GAL4^ap-md544

Rheb^Scer\UAS.cPa

wing | anterior compartment, with Scer\GAL4^ap-md544

Rheb^Scer\UAS.cPa

photoreceptor cell, with Scer\GAL4^GMR.PF

Rheb^Scer\UAS.cSa

salivary gland | conditional qualifier | somatic clone, with Scer\GAL4^GMR.PF

Rheb^Scer\UAS.cSa

fat body | conditional qualifier | somatic clone, with Scer\GAL4^GMR.PF

Rheb^Scer\UAS.cSa

mitotic cell cycle, with Scer\GAL4^Act5C.PP

Rheb^Scer\UAS.cSa

1st posterior cell, with Scer\GAL4^ptc-559.1

Rheb^Scer\UAS.cSa

G1 phase

Rheb^dsRNA.cPa

Classical Alleles ( 21 )

For All Classical Alleles Show

Allele of Rheb	Class	Mutagen	Stocks	Known lesion
Rheb^1TI		ethyl methanesulfonate	0	Yes
Rheb²⁰¹		ethyl methanesulfonate	0	Yes
Rheb^26.2			0	--
Rheb^2D1		ethyl methanesulfonate	0	--
Rheb^2G5		ethyl methanesulfonate	0	Yes
Rheb^3M2		ethyl methanesulfonate	0	--
Rheb^44.1		Δ2-3	0	Yes
Rheb^4L1		ethyl methanesulfonate	0	Yes
Rheb⁵⁰¹		ethyl methanesulfonate	0	Yes
Rheb⁵⁶		Δ2-3	0	Yes
Rheb^7A1		ethyl methanesulfonate	0	Yes
Rheb^AV4	loss of function	P-element activity	1	Yes
Rheb^{EP50.084-loxP}		cre recombinase	0	Yes
Rheb^EP50.084			0	Yes
Rheb^EY08085		P-element activity	1	--
Rheb^GSjE2		Δ2-3	0	Yes
Rheb^LA01053		P-element activity	1	--
Rheb^PΔ1		Δ2-3	0	Yes
Rheb^PΔ2		Δ2-3	0	Yes
Rheb^c02175		piggyBac transposase	1	--
Rheb^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Rheb	Mutagen	Stocks	Known lesion
Rheb^{Act5C.T:Hsap\MYC}	in vitro construct \| regulatory fusion	0	Yes
Rheb^{Act5C.T:Zzzz\FLAG}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
Rheb^{Act5C.cHa.T:Hsap\MYC}	in vitro construct \| coding region fusion	0	Yes
Rheb^GD13254	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
Rheb^Scer\UAS.cPa	in vitro construct \| regulatory fusion	2	Yes
Rheb^Scer\UAS.cSa	in vitro construct \| regulatory fusion	0	Yes
Rheb^dsRNA.cBa	in vitro construct \| RNAi	0	Yes
Rheb^dsRNA.cPa	in vitro construct \| RNAi	0	Yes
Rheb^t10.8	in vitro construct \| genomic fragment	1	Yes

Aneuploid Aberrations

Useful duplication

Dp(3;3)Tpl-T34

Disrupted in

Df(3R)Exel6144

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD13254}

P{UAS-Rheb.Pa}

P{UAS-Rheb.S}

characterization construct

P{PYD2+}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}Rheb^c02175

P{EP.Scer\UAS(loxP.Scer\UAS.y⁺).BN}Rheb^EP50.084

P{EP.Scer\UAS.(-loxP).BN}Rheb^{EP50.084-loxP}

P{SUPor-P}KG02006

insertion of mobile activating element

P{EPgy2}Rheb^EY08085

P{GSV1}Rheb^GSjE2

P{Mae-UAS.6.11}Rheb^AV4

P{Mae-UAS.6.11}Rheb^LA01053

Related Comments

Please look at the allele reports for the complete phenotype data

In mitotic tissues, overexpression of Rheb accelerates passage through G1-S phase without affecting rates of cell division, whereas in endoreplicating tissues, overexpression increases DNA ploidy. Overexpression of Rheb can also drive cell growth in starved animals.

(Saucedo et al., 2003)