Gene Dmel\lack

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General Information

Symbol

Dmel\lack

Species

D. melanogaster

Name

lethal with a checkpoint kinase

Annotation symbol

CG4943

Feature type

protein_coding_gene

FlyBase ID

FBgn0029006

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

54C12-54D1

Sequence location

2R:13,480,319..13,491,489 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene lethal with a checkpoint kinase is referred to in FlyBase by the symbol lack (CG4943, FBgn0029006). It has the cytological map location 54C12-54D1. Its sequence location is 2R:13480319..13491489. Its molecular function is described as: ubiquitin-protein ligase activity; specific transcriptional repressor activity; protein binding. It is involved in the biological processes: ubiquitin cycle; transforming growth factor beta receptor signaling pathway; regulation of growth. 9 alleles are reported. The phenotypes of these alleles are annotated with: eye; embryonic/first instar larval cuticle; embryonic head; amnioserosa; embryonic hindgut; stomodeum; wing; leg. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

54C12-54D1

Limits computationally determined from genome sequence between P{PZ}l(2)10505¹⁰⁵⁰⁵ and P{lacW}l(2)k07509^k07509&P{EP}CG10934^EP538

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

54C-54D

(determined by in situ hybridisation)

(Podos, 2000.3.18)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\lack for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

lack-RA

FBtr0086833

NM_079055

4865

1061

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

lack-PA

FBpp0086011

115.7

1061

9.30

NP_523779

AAF57824

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0029006

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

C2 calcium-dependent membrane targeting (IPR000008)

HECT (IPR000569)

WW/Rsp5/WWP (IPR001202)

C2 calcium/lipid-binding region, CaLB (IPR008973)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V853

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12063

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI543411
AA978849
AA951014

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0029006

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0029006

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0029006

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

lethal | rescuable maternal effect

lack^15C

lethal | partially | rescuable maternal effect

lack^11R

lethal | pharate adult stage, with Scer\GAL4^ptc-559.1

lack^{Scer\UAS.T:Zzzz\FLAG}

Other Phenotypes

Allele

visible | recessive | somatic clone

lack^KG07014

Sterility

Allele

fertile

Phenotype manifest in

Allele

embryonic/first instar larval cuticle | rescuable maternal effect

embryonic head | rescuable maternal effect

lack^15C

amnioserosa | rescuable maternal effect

lack^15C

embryonic hindgut | rescuable maternal effect

lack^15C

stomodeum | dorsal | rescuable maternal effect

lack^15C

eye | somatic clone

lack^KG07014

wing, with Scer\GAL4^ptc-559.1

lack^{Scer\UAS.T:Zzzz\FLAG}

leg, with Scer\GAL4^ptc-559.1

lack^{Scer\UAS.T:Zzzz\FLAG}

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of lack	Mutagen	Stocks	Known lesion
lack^11R	hobo activity	0	Yes
lack^15C	hobo activity	0	Yes
lack^KG07014	P-element activity	1	--
lack^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of lack	Mutagen	Stocks	Known lesion
lack^{C1029A.Scer\UAS.T:Zzzz\FLAG}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion in vitro construct \| amino acid replacement	0	Yes
lack^GD11480	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
lack^{MtnA.T:Hsap\MYC}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
lack^{Scer\UAS.T:Zzzz\FLAG}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
lack^dsRNA.si.cLa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful duplication

Dp(2;Y)53D;57C

Disrupted in

Df(2R)Exel7149

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11480}

P{UAS-lack.FLAG.C1029A}

P{UAS-lack.FLAG}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

H{}lack^11R

H{}lack^15C

P{SUPor-P}lack^KG07014

insertion of enhancer trap

P{lacW}l(2)SH0991^SH0991

Related Comments

Please look at the allele reports for the complete phenotype data

Embryos lacking both maternal and zygotic lack function die with an apparently normal dorsal-ventral pattern but with a cuticular phenotype. The lethal phenotype is suppressed by a reduction in dpp⁺ gene dosage.

(Podos and Ferguson, 2000)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

protein binding

inferred from electronic annotation with InterPro:IPR001202

(FlyBase Curators et al., 2004-)

specific transcriptional repressor activity

inferred from mutant phenotype

(Anderson et al., 2006)