Gene Dmel\armi

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General Information

Symbol

Dmel\armi

Species

D. melanogaster

Name

armitage

Annotation symbol

CG11513

Feature type

protein_coding_gene

FlyBase ID

FBgn0041164

Created / Updated

2005-01-10/2005-01-10

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

63E1-63E1

Sequence location

3L:3,461,323..3,466,314 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene armitage is referred to in FlyBase by the symbol armi (CG11513, FBgn0041164). It has the cytological map location 63E1. Its sequence location is 3L:3461323..3466314. Its molecular function is described as DNA helicase activity. It is involved in the biological processes: embryonic axis specification; mRNA catabolic process, nonsense-mediated decay; negative regulation of oskar mRNA translation; regulation of pole plasm oskar mRNA localization; RNA interference; dorsal appendage formation; pole plasm protein localization; microtubule organizing center organization and biogenesis; long-term memory; oocyte microtubule cytoskeleton organization. 8 alleles are reported. The phenotypes of these alleles are annotated with: dorsal appendage; egg. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63E1-63E1

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: armi? CycJ?

(Theurkauf, 2000.3.3)

Overall orientation not stated: armi+ CycJ+

(Cook et al., 2004)

Gene Model & Products

Please see the GBrowse view of Dmel\armi for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

armi-RA

FBtr0073126

NM_139559

4081

1274

armi-RB

FBtr0100641

NM_001014556

4117

1188

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

armi-PA

FBpp0100101

144.9

1274

5.69

NP_647816

AAF47775

armi-PB

FBpp0100102

135.7

1188

6.09

NP_001014556

AAX52729

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q6J5K9

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

GM10845

(Cook et al., 2004)

GM12116

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2239

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0041164

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

memory defective (with Df(3L)E1)

armi^72.1

memory defective | recessive

armi^72.1

Sterility

Allele

female sterile

male sterile | partially

Phenotype manifest in

Allele

dorsal appendage | maternal effect

dorsal appendage (with armi^72.1)

armi¹

microtubule organizing center & oocyte (with armi^72.1)

armi¹

egg | maternal effect

armi^72.1

oocyte & microtubule organizing center

armi^72.1

oocyte & microtubule

armi^72.1

dorsal appendage (with armi¹)

armi^72.1

microtubule organizing center & oocyte (with armi¹)

armi^72.1

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of armi	Class	Mutagen	Stocks	Known lesion
armi¹			1	Yes
armi^72.1	loss of function	Δ2-3	1	Yes
armi^KG04664		P-element activity	1	--
armi^f02594		piggyBac transposase	0	--
armi^rev39.2		Δ2-3	0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of armi	Mutagen	Stocks	Known lesion
armi^GD7319	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
armi^{Scer\UAS.P\T.T:Avic\GFP-EGFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	2	Yes
armi^dsRNA.cDa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)1227

Useful duplication

Dp(3;3)SMG38

Disrupted in

Df(3L)E1

(Cook et al., 2004)

Df(3L)Exel6095

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7319}

P{UASp-armi-EGFP}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}armi^f02594

P{SUPor-P}armi^KG04664

insertion of enhancer trap

P{lacW}armi¹

Related Comments

Please look at the allele reports for the complete phenotype data

Mutations in armi block posterior migration of the microtubule organising centre and cause defects in RNA localisation in the oocyte.

(Cook and Theurkauf, 2000)

armi mutants are impaired in a step in RNA-induced silencing complex (RISC) assembly after binding of the siRNA to the Dcr-2/r2d2 protein heterodimer.

(Tomari et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 16 )

Molecular Function

CV term

Evidence

References

DNA helicase activity

non-traceable author statement

(Tomari et al., 2004)

Biological Process

CV term

Evidence

References

RNA interference