A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\nht

General Information
SymbolDmel\nhtSpeciesD. melanogaster
Nameno hitterAnnotation symbolCG15259
Feature typeprotein_coding_geneFlyBase IDFBgn0041103
Created / Updated2003-12-01/2008-06-17
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map35D1-35D1Sequence location2L:15,317,954..15,318,901 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene no hitter is referred to in FlyBase by the symbol nht (CG15259, FBgn0041103). It has the cytological map location 35D1. Its sequence location is 2L:15317954..15318901. Its molecular function is described as: general RNA polymerase II transcription factor activity; transcription initiation factor activity. It is involved in the biological processes: transcription initiation from RNA polymerase II promoter; spermatogenesis. 6 alleles are reported. The phenotypes of these alleles are annotated with: spermatocyte; testis. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
35D1-35D1  
Limits computationally determined from genome sequence between P{EP}vigEP812&P{PZ}stc05441 and P{EP}esgEP633&P{PZ}esg07082  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
35C-35C
 
(Hiller, 2000.3.10)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: CG15262+ nht- esg+
(Ashburner et al., 1999)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\nht for information on other features
detailed view FBtr0300021 FBpp0289298
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
nht-RB
FBtr0300021
 
  948
  245
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
nht-PB  
FBpp0289298  
28.1  
245  
8.44  
 
 
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Transcription initiation factor TFIID component TAF4 (IPR007900)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AE003412
AAF44939
 
AE003647
AAF53457
 
AY752877
AAV33342
 
BT022194
 
CO193497
 
L80117
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    (Ashburner et al., 1999.10.12)
    Does NOT map to
    Identified with
    (Hoskins et al., 2005)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    nht[z-5946]
    2L:15,318,801..15,318,801
    comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. FlyBase protein as of July 2006 is incorrect (wrong start Met) and is awaiting update. Protein produced by cDNA AY752877 is correct.
    evidence=experimental
    na_change=C15318801T
    pr_change=Q16@|nht-PA
    reported_na_change=C114T
    reported_pr_change=Q16@
    (Hiller et al., 2004)
    point mutation
    nht[z-5347]
    2L:15,318,350..15,318,350
    comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. FlyBase protein as of July 2006 is incorrect (wrong start Met) and is awaiting update. Protein produced by cDNA AY752877 is correct.
    evidence=experimental
    na_change=C15318350A
    pr_change=S108@|nht-PA
    reported_na_change=C566A
    reported_pr_change=S166@
    (Hiller et al., 2004)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Other Phenotypes
        Allele
        meiotic cell cycle defective
        meiotic cell cycle defective (with Df(2L)A263)
        Sterility
        Allele
        male sterile
        male sterile (with Df(2L)A263)
        male sterile | recessive
        Phenotype manifest in
        Allele
        spermatocyte (with Df(2L)A263)
        testis (with Df(2L)A263)
        hide Classical Alleles ( 4 )
        For All Classical Alleles Show

        Allele of nhtClassMutagenStocksKnown lesion
        nhtZ2-5946
          		0 --
          nhtz-53470 Yes
          nhtz-59460 Yes
          nhtz5347
            		0 --
            hide Alleles Carried on Transgenic Constructs ( 2 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of nhtClassMutagenStocksKnown lesion
            nht+t10.50 Yes
            nhtGD40651 Yes
            hide Aneuploid Aberrations
            Useful deficiency
             
             
            Useful duplication
             
            Disrupted in
            (Ashburner et al., 1999.10.12, Hiller et al., 2004)
            (Roote, 2002.2.6, Ryder et al., 2004, Ryder and Roote, 2004.11.10)
            (Ryder, 2004.4.26)
            (Ryder, 2004.4.26)
            (Hiller et al., 2004)
            (Ashburner et al., 1999.10.12)
            (Hiller et al., 2004)
            Not disrupted in
            (Hiller et al., 2004)
            (Hiller et al., 2004)
            (Hiller et al., 2004)
            (Hiller et al., 2004)
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            UAS construct
            P{GD4065}
            NA
            characterization construct
            P{nht.10.5}
            NA
            Insertions
            Type of insertions
            Name
            Expression data
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            hide Gene Ontology: Function, Process & Cellular Component ( 5 )
            hide Molecular Function
            CV term
            Evidence
            References
            general RNA polymerase II transcription factor activity
            inferred from sequence or structural similarity with HGNC_gene:TAF4; HGNC:11537; OMIM:601796 AND inferred from sequence or structural similarity with HGNC_gene:TAF4B; HGNC:11538; OMIM:601689 AND inferred from sequence or structural similarity with SGD_LOCUS:MPT1; SGD:S0004607
            (Aoyagi and Wassarman, 2000)
            transcription initiation factor activity
            inferred from electronic annotation with InterPro:IPR007900
            (FlyBase Curators et al., 2004-)
            hide Biological Process
            CV term
            Evidence
            References
            spermatogenesis
            inferred from mutant phenotype
            (Hiller et al., 2004)
            transcription initiation from RNA polymerase II promoter
            inferred from sequence or structural similarity with HGNC_gene:TAF4; HGNC:11537; OMIM:601796 AND inferred from sequence or structural similarity with HGNC_gene:TAF4B; OMIM:601689 AND inferred from sequence or structural similarity with SGD_LOCUS:MPT1; SGD:S0004607
            (Aoyagi and Wassarman, 2000)
            hide Cellular Component
            CV term
            Evidence
            References
            transcription factor TFIID complex
            inferred from sequence or structural similarity with HGNC_gene:TAF4; HGNC:11537; OMIM:601796 AND inferred from sequence or structural similarity with HGNC_gene:TAF4B; HGNC:11538; OMIM:601689 AND inferred from sequence or structural similarity with SGD_LOCUS:MPT1; SGD:S0004607
            (Aoyagi and Wassarman, 2000)
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            nht allele
            Gene
            References
            nhtz5347
            ms(2)35Ci
            (Ashburner et al., 1999.10.12)
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
             
            Linkouts
            hide Functional Complementation between Species
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 1 )
            VDRC
            v10351
            w1118; P{GD4065}v10351
            hide Genomic Clones ( 1 )
            hide cDNA Clones ( 2 )
            Please Note
            This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            hide Antibody Information
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for database merge of
            Source for merge of: nht BG:DS07851.6
            (Aoyagi and Wassarman, 2000)
            Additional comments
            hide Comments About Role
            hide Comments About Molecular Function
            nht is only expressed in primary spermatocytes, but not in females or in somatic tissues. nht regulates the transcription of a specific set of genes in the primary spermatocyte.
            (Hiller et al., 2000)
            hide Other Comments
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            DDBJ / EMBL / GenBank
            •
            UniProtKB/TrEMBL
            •
            Other Crossreferences
            InterPro domains - A database of protein families, domains, and functional sites
            •
            Transcription initiation factor TFIID component TAF4 (IPR007900)
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            DEDB - Drosophila exon database: splicing graphs
            •
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            •
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            •
            FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
            •
            GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            PANTHER - Protein classification by function, families, and pathways
            •
            hide Synonyms & Secondary IDs ( 11 )
            Reported As
            Symbol Synonym
            BG:DS07851.6
            (Ashburner et al., 1999.10.12, Ashburner et al., 1999, Ashburner et al., 1999, )
            CG15259
            (Aoyagi and Wassarman, 2000, )
            dmTAF4b
            (Hochheimer and Tjian, 2003)
            dTAF4L
            (Hiller et al., 2004)
            dTAFII110
            (Hochheimer and Tjian, 2003)
            Nht
            (Mueller and Tora, 2004)
            nht
            (Ceulemans, 2006, Mikhaylova et al., 2006, Hoskins et al., 2005, Jiang et al., 2007, Chen et al., 2005, Chen et al., 2005)
            TAF4
            (Hiller et al., 2004.9.14)
            TFIID
            (Butler and Kadonaga, 2002)
            Name Synonym
            nohitter
            (Hochheimer and Tjian, 2003)
            no hitter
            (Hiller et al., 2000)
            Secondary FlyBase IDs
            • FBgn0028851
            hide References ( 34 )
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            hide Recent research papers ( 2 )
            Jiang et al., 2007, Development Suppl.:
            Tombola, a tesmin/TSO1-family protein, regulates transcriptional activation in the Drosophila male germline and physically interacts with Always early. [FBrf0194011]
            Mikhaylova et al., 2006, Proc. Natl. Acad. Sci. USA 103(32): 11975--11980
            Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line. [FBrf0195382]
            Show all research papers ...
            hide Recent reviews ( 1 )
            Ceulemans, 2006, BioEssays 28(3): 316--324
            [FBrf0198745]
            Show all reviews ...