A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\MTF-1

General Information
SymbolDmel\MTF-1SpeciesD. melanogaster
NameMetal response element-binding Transcription Factor-1Annotation symbolCG3743
Feature typeprotein_coding_geneFlyBase IDFBgn0040305
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map67B5-67B6Sequence location3L:9,423,442..9,431,809 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Metal response element-binding Transcription Factor-1 is referred to in FlyBase by the symbol MTF-1 (CG3743, FBgn0040305). It has the cytological map location 67B5-67B6. Its sequence location is 3L:9423442..9431809. Its molecular function is described as: specific RNA polymerase II transcription factor activity; transcription factor activity; zinc ion binding. It is involved in the biological processes: cellular metal ion homeostasis; response to copper ion; response to metal ion; transcription. 13 alleles are reported. No phenotypic data is available. It has 5 annotated transcripts and 5 annotated polypeptides.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
67B5-67B6  
Limits computationally determined from genome sequence between P{EP}Hsp26EP3336&P{EP}Hsp26EP3315 and P{PZ}fry02240  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
Overall orientation not stated: aay+ Shc+ RpS17- MTF-1+
(Georgiev, 2000.2.3)
hide Gene Model & Products
Please see the GBrowse view of Dmel\MTF-1 for information on other features
detailed view FBtr0076458 FBtr0076479 FBtr0113322 FBtr0113323 FBtr0076459 FBtr0076460 FBtr0113324 FBtr0089879 FBtr0076504 FBpp0076186 FBpp0076207 FBpp0076187 FBpp0112234 FBpp0112235 FBpp0112236 FBpp0076188 FBpp0076232 FBpp0088818 FBti0039159 FBti0053430 FBti0059121
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
MTF-1-RA
FBtr0076459
 NM_140054
  3343
  791
MTF-1-RB
FBtr0076460
 NM_168343
  3240
  570
MTF-1-RC
FBtr0113322
 NM_001104090
  3988
  1006
MTF-1-RD
FBtr0113323
 NM_001104091
  3840
  570
MTF-1-RE
FBtr0113324
 NM_001104092
  2817
  570
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
MTF-1-PA  
FBpp0076187  
85.2  
791  
6.46  
  NP_648311
  AAF50271
MTF-1-PB  
FBpp0076188  
60.1  
570  
5.66  
  NP_729491
  AAN11970
MTF-1-PC  
FBpp0112234  
108.6  
1006  
4.89  
  NP_001097560
  ABW08503
MTF-1-PD  
FBpp0112235  
60.1  
570  
5.66  
  NP_001097561
  ABW08504
MTF-1-PE  
FBpp0112236  
60.1  
570  
5.66  
  NP_001097562
  ABW08505
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Zinc finger, C2H2-type (IPR007087)
Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087)
Zinc finger, C2H2-like (IPR015880)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AE003552
AAF50271
 
AE003552
AAN11970
 
AI062662
 
AI532170
 
AI544333
 
AJ271817
CAB72252
 
AJ297844
CAC14279
 
AW944665
 
AX094287
 
AY058796
AAL14025
 
BG639624
 
BI354397
 
BI481796
 
BI637813
 
BT023803
 
BT025220
 
BT029122
 
CK659673
 
CO273150
 
CO273163
 
CO315282
 
CZ490066
 
EC080203
 
EC197975
 
EC197977
 
EC215982
 
EC225792
 
EC238395
 
EC249688
 
EL884489
 
EL884490
 
EL884616
 
EL884617
 
EL884724
 
EL884725
 
SD03560
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    rescue fragment
    MTF-1[+tEa]
    3L:9,417,368..9,434,368
    comment=The 5' end of the rescue fragment is approximate. It was reported as a 17kb NotI fragment, however the 5' NotI site is not present in the reference genomic DNA.
    evidence=experimental
    linked_to=NotI_rfrag
    (Egli et al., 2003)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0076458 FBtr0076479 FBtr0113322 FBtr0113323 FBtr0076459 FBtr0076460 FBtr0113324 FBtr0089879 FBtr0076504
        AI544333
        AI532170
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for MTF-1
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for MTF-1
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        viable (with Df(3L)AC1)
        lethal | third instar larval stage
        Other Phenotypes
        Allele
        chemical sensitive
        chemical sensitive | recessive
        developmental rate defective
        Sterility
        Allele
        fertile
        fertile (with Df(3L)AC1)
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 6 )
        For All Classical Alleles Show

        Allele of MTF-1ClassMutagenStocksKnown lesion
        MTF-11-1
          		0 --
          MTF-1140-1R1 Yes
          MTF-153-1R0 Yes
          MTF-161-2R0 Yes
          MTF-1f072551 --
          MTF-1unspecified
            		0 --
            hide Alleles Carried on Transgenic Constructs ( 7 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of MTF-1ClassMutagenStocksKnown lesion
            MTF-1+tEa0 Yes
            MTF-1GD161661 Yes
            MTF-1GD41142 Yes
            MTF-1Scer\UAS.P\T.cBa0 Yes
            MTF-1Scer\UAS.cEa0 Yes
            MTF-1Tub.PY0 Yes
            MTF-1mutATG.Scer\SceI.RS0 --
            hide Aneuploid Aberrations
            Useful deficiency
             
            Useful duplication
             
            Disrupted in
            (Egli et al., 2003)
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            UAS construct
            P{GD16166}
            NA
            P{GD4114}
            NA
            P{UAS-MTF-1.E}
            NA
            P{UASp-MTF-1.B}
            NA
            characterization construct
            P{aj271817}
            NA
            Insertions
            Type of insertions
            Name
            Expression data
            miscellaneous insertions
            PBac{WH}MTF-1f07255
            NA
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            S2 cells treated with MTF-1 dsRNA show reduced tolerance to increased copper in the culture medium.
            (Southon et al., 2004)
            hide Gene Ontology: Function, Process & Cellular Component ( 10 )
            hide Molecular Function
            CV term
            Evidence
            References
            specific RNA polymerase II transcription factor activity
            non-traceable author statement
            (Georgiev, 2000.2.3)
            non-traceable author statement
            (Georgiev, 2000.10.20)
            transcription factor activity
            non-traceable author statement
            (Georgiev, 2000.10.20)
            inferred from direct assay
            (Marr et al., 2006)
            zinc ion binding
            inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880
            (FlyBase Curators et al., 2004-)
            hide Biological Process
            CV term
            Evidence
            References
            cellular metal ion homeostasis
            inferred from mutant phenotype
            (Egli et al., 2003)
            response to copper ion
            inferred from mutant phenotype
            (Southon et al., 2004)
            response to metal ion
            inferred from mutant phenotype
            (Selvaraj et al., 2005)
            transcription
            non-traceable author statement
            (Georgiev, 2000.10.20)
            hide Cellular Component
            CV term
            Evidence
            References
            cytoplasm
            inferred from direct assay
            (Marr et al., 2006)
            nucleoplasm
            inferred from electronic annotation
            (Roth and Foulger, 2002.4.24)
            nucleus
            non-traceable author statement
            (Georgiev, 2000.2.3)
            non-traceable author statement
            (Georgiev, 2000.10.20)
            inferred from direct assay
            (Marr et al., 2006)
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            MTF-1 allele
            Gene
            References
            unspecified
            Ctr1B
            (Egli et al., 2006)
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
             
            Linkouts
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Functional Complementation between Species
            Partially functionally complements
            Hsap\MTF1
            (Balamurugan et al., 2004)
            Partially functionally complemented by
            Hsap\MTF1
            (Balamurugan et al., 2004)
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 5 )
            Bloomington
            9241
            MTF-1140-1R
            Harvard
            -
            PBac{WH}MTF-1f07255
            VDRC
            v30455
            w1118; P{GD4114}v30455
            v43939
            w1118; P{GD4114}v43939
            v48822
            w1118; P{GD16166}v48822
            hide Genomic Clones ( 1 )
            hide cDNA Clones ( 15 )
            Please Note
            This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            hide Antibody Information
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for database merge of
            Source for merge of: MTF-1 CG3743
            (Bayraktaroglu, 2000.8.7)
            Source for merge of: MTF-1 anon-WO0118547.411 Source for merge of MTF-1 anon-WO0118547.411 was sequence comparison (date:051113).
            (FlyBase, 1996-)
            Additional comments
            hide Comments About Role
            MTF-1 is essential for the control of heavy metal homeostasis.
            (Egli et al., 2003)
            Regulation of Ctr1B upon copper starvation is lost in the absence of MTF-1.
            (Selvaraj et al., 2005)
            hide Comments About Molecular Function
            hide Other Comments
            Area matching Drosophila EST AI532170. Forms a 1565bp EST contig with AI544333 and AI062662, sequence similarity to MTF-1 gene. Probable intron in gene represented by EST AI532170.
            (Davies, 2001.3.15)
            dsRNA made from templates generated with primers directed against this gene used to investigate the role of this gene in copper homoeostasis in S2 cells.
            (Southon et al., 2004)
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            RefSeq (Transcripts)
            •
            RefSeq (Proteins)
            •
            DDBJ / EMBL / GenBank
            •
            UniProtKB/TrEMBL
            •
            Other Crossreferences
            InterPro domains - A database of protein families, domains, and functional sites
            •
            Zinc finger, C2H2-type (IPR007087)
            Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087)
            Zinc finger, C2H2-like (IPR015880)
            Linkouts
            BDGP expression data
            •
            Show all BDGP original images, annotations and embryonic microarray data for MTF-1
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            DEDB - Drosophila exon database: splicing graphs
            •
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            •