A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\POSH

General Information
SymbolDmel\POSHSpeciesD. melanogaster
NamePlenty of SH3sAnnotation symbolCG4909
Feature typeprotein_coding_geneFlyBase IDFBgn0040294
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)2RRecombination map
Cytogenetic map54C9-54C9Sequence location2R:13,456,243..13,459,827 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Plenty of SH3s is referred to in FlyBase by the symbol POSH (CG4909, FBgn0040294). It has the cytological map location 54C9. Its sequence location is 2R:13456243..13459827. Its molecular function is described as: receptor signaling complex scaffold activity; protein binding; zinc ion binding. It is involved in the biological processes: determination of adult life span; oocyte microtubule cytoskeleton polarization; pole plasm oskar mRNA localization; protein localization; rhabdomere development. 10 alleles are reported. The phenotypes of these alleles are annotated with: wing hair; genitalia; tarsal segment; macrochaeta; wing; eye. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
54C9-54C9  
Limits computationally determined from genome sequence between P{EP}MESR4EP386&P{EP}POSHEP1206 and P{PZ}l(2)1050510505  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
54C7-54C8
(determined by in situ hybridisation)  
(Spradling et al., 1999)
54C1-54C2
54C1--4 54C5--8 54C7--8  
(BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\POSH for information on other features
detailed view FBtr0086906 FBtr0086828 FBtr0086905 FBtr0290307 FBtr0086831 FBtr0086829 FBtr0086830 FBpp0086063 FBpp0086007 FBpp0086062 FBpp0288746 FBpp0086009 FBpp0086008 FBpp0089285 FBti0010972 FBti0007791 FBti0006382 FBti0039021 FBti0025056 FBti0049833
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
POSH-RA
FBtr0086828
 NM_079052
  3163
  838
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
POSH-PA  
FBpp0086007  
92.1  
838  
9.33  
  NP_523776
  AAF57833
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Zinc finger, RING/FYVE/PHD-type (IPR013083)
Neutrophil cytosol factor 2 (IPR000108)
Src homology-3 (IPR001452)
Zinc finger, RING-type (IPR001841)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA696849
 
AA697215
 
AA802398
 
AE003802
AAF57833
 
AF220364
AAF37265
 
AI513167
 
AI534514
 
AQ025053
 
AQ026092
 
AQ034122
 
AW943782
 
AY051984
AAK93408
 
BF492022
 
BI231608
 
BI232744
 
BI374994
 
CK131600
 
CL526219
 
CO177832
 
CO266204
 
CO311956
 
CO327312
 
CO327744
 
CO332453
 
CO341188
 
CS015727
 
EC061489
 
EC063998
 
EC067820
 
EC087720
 
EC210380
 
EC213603
 
EC246736
 
EC254067
 
EC254157
 
EC255934
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Toba et al., 1999, Spradling et al., 1999)
    (BDGP Project Members, 2000-, Seong et al., 2002)
    (Seong et al., 2002)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage | early
     
    (Seong et al., 2002)
     larval stage | third instar stage 2
     dorsal mesothoracic disc & ventral thoracic disc
    (Seong et al., 2002)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0086906 FBtr0086828 FBtr0086905 FBtr0290307 FBtr0086831 FBtr0086829 FBtr0086830
        AI513167
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        lethal | recessive
        lethal, with Scer\GAL429BD
        Other Phenotypes
        Allele
        wild-type, with Scer\GAL4dpp.blk1
        visible, with Scer\GAL4c355
        visible, with Scer\GAL4hs.2sev
        visible, with Scer\GAL4GMR.PF
        long lived, with Scer\GAL4hs.PB
        long lived, with Scer\GAL4Appl.G1a
        tissue polarity defective, with Scer\GAL4ptc-559.1
        Sterility
        Allele
        fertile
        Phenotype manifest in
        Allele
        wing hair, with Scer\GAL4ptc-559.1
        genitalia, with Scer\GAL4ptc-559.1
        tarsal segment, with Scer\GAL4ptc-559.1
        macrochaeta, with Scer\GAL4ptc-559.1
        wing, with Scer\GAL4c355
        eye, with Scer\GAL4hs.2sev
        eye, with Scer\GAL4GMR.PF
        hide Classical Alleles ( 8 )
        For All Classical Alleles Show

        Allele of POSHClassMutagenStocksKnown lesion
        POSHEP12062 --
        POSHEP22482 --
        POSHEY103541 --
        POSHGS22071 Yes
        POSHk062021 --
        POSHk115071 Yes
        POSHk155121 --
        POSHk158152 Yes
        hide Alleles Carried on Transgenic Constructs ( 2 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of POSHClassMutagenStocksKnown lesion
        POSHGD114672 Yes
        POSHScer\UAS.cSa0 Yes
        hide Aneuploid Aberrations
        Useful duplication
         
        Not disrupted in
        (Spradling et al., 1999, BDGP Project Members, 1994-1999)
        Disrupted in
        (Deal-Herr and Cook, 2003.12.11)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD11467}
        NA
        P{UAS-POSH.S}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        insertion of enhancer trap
        P{lacW}POSHk06202
        No
        P{lacW}POSHk11507
        No
        P{lacW}POSHk15512
        No
        P{lacW}POSHk15815
        No
        insertion of mobile activating element
        P{EPgy2}POSHEY10354
        NA
        P{EP}POSHEP1206
        NA
        P{EP}POSHEP2248
        NA
        P{GSV1}POSHGS2207
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        hide Gene Ontology: Function, Process & Cellular Component ( 8 )
        hide Molecular Function
        CV term
        Evidence
        References
        protein binding
        inferred from electronic annotation with InterPro:IPR001841
        (FlyBase Curators et al., 2004-)
        receptor signaling complex scaffold activity
        non-traceable author statement
        (Aigaki et al., 2002)
        zinc ion binding
        inferred from electronic annotation with InterPro:IPR001841
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        determination of adult life span
        inferred from mutant phenotype
        (Seong et al., 2002)
        non-traceable author statement
        (Aigaki et al., 2002)
        oocyte microtubule cytoskeleton polarization
        inferred from mutant phenotype
        (Xiao et al., 2002)
        pole plasm oskar mRNA localization
        inferred from mutant phenotype
        (Xiao et al., 2002)
        protein localization
        inferred from mutant phenotype
        (Xiao et al., 2002)
        rhabdomere development
        inferred from mutant phenotype
        (Xiao et al., 2002)
        hide Cellular Component
        CV term
        Evidence
        References
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        POSH allele
        Gene
        References
        POSHk11507
        Ras85D
        (Schnorr et al., 2001)
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 13 )
        Szeged
        -
        P{EP}POSHEP2248
        -
        P{EP}POSHEP1206
        Kyoto
        200330
        P{GSV1}POSHGS2207
        102452
        Df(1)w67c23, y1 ; P{lacW}POSHk06202 / CyO
        102809
        Df(1)w67c23, y1 ; P{lacW}POSHk11507 / CyO
        102965
        Df(1)w67c23, y1 ; P{lacW}POSHk15512 / CyO
        102970
        Df(1)w67c23, y1 ; P{lacW}POSHk15815 / CyO
        VDRC
        v26655
        w1118; P{GD11467}v26655
        v26657
        w1118; P{GD11467}v26657
        Bloomington
        17036
        w1118; P{EP}POSHEP2248
        17671
        y1 w67c23; P{EPgy2}POSHEY10354
        11146
        y1 w67c23; P{lacW}POSHk15815/CyO
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 17 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for database merge of
        Source for merge of: POSH CG4909
        (Bayraktaroglu, 2000.8.7)
        Source for merge of: POSH l(2)k15815
        (Schnorr et al., 2001)
        Additional comments
        hide Comments About Role
        POSH has a role in longevity determination.
        (Seong et al., 2002)
        hide Comments About Molecular Function
        hide Other Comments
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •
        UniProtKB/TrEMBL
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Zinc finger, RING/FYVE/PHD-type (IPR013083)
        Neutrophil cytosol factor 2 (IPR000108)
        Src homology-3 (IPR001452)
        Zinc finger, RING-type (IPR001841)
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        DEDB - Drosophila exon database: splicing graphs
        •
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        InParanoid orthologs - Eukaryotic orthologs
        •
        PANTHER - Protein classification by function, families, and pathways
        •
        University of Chicago Dev. Expression
        •
        hide Synonyms & Secondary IDs ( 10 )
        Reported As
        Symbol Synonym
        155116_at
        (Kristensen et al., 2005)
        BEST:GM09451
         
        CG4909
        (Seong et al., 2002, )