Gene Dmel\Best1

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General Information

Symbol

Dmel\Best1

Species

D. melanogaster

Name

Bestrophin 1

Annotation symbol

CG6264

Feature type

protein_coding_gene

FlyBase ID

FBgn0040238

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

85F15-85F16

Sequence location

3R:5,987,186..5,996,207 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Bestrophin 1 is referred to in FlyBase by the symbol Best1 (CG6264, FBgn0040238). It has the cytological map location 85F15-85F16. Its sequence location is 3R:5987186..5996207. Its molecular function is described as chloride channel activity. The biological processes in which it is involved are not known. 12 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85F15-85F16

Limits computationally determined from genome sequence between P{EP}Fmr1^EP3517&P{PZ}tws⁰²⁴¹⁴ and P{PZ}jumu⁰⁶⁴³⁹&P{lacW}l(3)j8B6^j8B6

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

85F-86A

(Tavsanli, 1999.12.23)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Best1 for information on other features

Comments on Gene Model

DGC clone LP05915 appears problematic: unspliced intron

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Best1-RA

FBtr0082183

NM_144346

3123

721

Best1-RB

FBtr0114590

3267

769

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Best1-PA

FBpp0081661

79.6

721

5.15

NP_652603

AAF54503

Best1-PB

FBpp0113082

84.3

769

4.88

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0040238

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Bestrophin (IPR000615)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V3J6

Maps to

Does NOT map to

Identified with

LP05915

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8903

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI257517

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0040238

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0040238

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0040238

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Sterility

Allele

fertile

Phenotype manifest in

Allele

Classical Alleles ( 7 )

For All Classical Alleles Show

Allele of Best1	Mutagen	Stocks	Known lesion
Best1^1-2	Δ2-3	0	Yes
Best1^3-4	Δ2-3	0	Yes
Best1^3-9	Δ2-3	0	Yes
Best1^CB02354	P-element activity	0	Yes
Best1^c04106	piggyBac transposase	1	--
Best1^e01309	piggyBac transposase	1	--
Best1^f07188	piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Best1	Mutagen	Stocks	Known lesion
Best1^GD2894	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Best1^{R218C.Scer\UAS.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement in vitro construct \| coding region fusion	0	Yes
Best1^{Scer\UAS.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
Best1^{T6P.Scer\UAS.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement in vitro construct \| coding region fusion	0	Yes
Best1^{W93C.Scer\UAS.T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)by77

Useful duplication

Dp(3;3)M86D⁺2

Disrupted in

Df(3R)Exel6265

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD2894}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}Best1^c04106

PBac{RB}Best1^e01309

PBac{WH}Best1^f07188

P{PTT-GB}Best1^CB02354

insertion of mobile activating element

P{EP}31-1

P{EP}EP3668

Related Comments

Please look at the allele reports for the complete phenotype data

Homozygous null mutants have normal viability and fertility and have normal photoreceptor morphology and function.

(Tavsanli et al., 2001)

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

chloride channel activity

non-traceable author statement

(Sun, 2004.1.2)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Best1 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

72719

Orthologs

Genome-wide drosophilid orthologs

Dana\GF17535

Dere\GG17296

Dgri\Rfp1

Dmoj\GI24037

Dper\GL23840

Dpse\GA19476

Dsec\GM26180

Dsim\GD20729

Dvir\GJ23667

Dwil\GK22536

Dyak\GE24699

Curated drosophilid orthologs

Dpse\GA19476

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000014086
Anopheles gambiae (Ensembl)
ENSBTAP00000003810
Bos taurus (Ensembl)
ENSCAFP00000025331
Canis familiaris (Ensembl)
ENSCINP00000014004
Ciona intestinalis (Ensembl)
NEWSINFRUP00000151123
Takifugu rubripes (Ensembl)
ENSGALP00000011669
Gallus gallus (Ensembl)
ENSP00000042931
Homo sapiens (Ensembl)
ENSMMUP00000006359
Macaca mulatta (Ensembl)
ENSMODP00000009399
Monodelphis domestica (Ensembl)
ENSPTRP00000006462
Pan troglodytes (Ensembl)
ENSRNOP00000004973
Rattus norvegicus (Ensembl)
GSTENP00022642001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000014724
Xenopus tropicalis (Ensembl)
WBGene00029215
Caenorhabditis briggsae (Wormbase)
WBGene00015286
Caenorhabditis elegans (Wormbase)
WBGene00022797
Caenorhabditis elegans (Wormbase)
MGI:2387588
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

v5963

w¹¹¹⁸; P{GD2894}v5963/TM3

Bloomington

19055

w¹¹¹⁸; PBac{WH}Best1^f07188/TM6B, Tb¹

Genomic Clones ( 2 )

cDNA Clones ( 37 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones