A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\dyn-p25

General Information
SymbolDmel\dyn-p25SpeciesD. melanogaster
Namedynactin-subunit-p25Annotation symbolCG10846
Feature typeprotein_coding_geneFlyBase IDFBgn0040228
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map35B8-35B8Sequence location2L:15,004,021..15,006,614 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene dynactin-subunit-p25 is referred to in FlyBase by the symbol dyn-p25 (CG10846, FBgn0040228). It has the cytological map location 35B8. Its sequence location is 2L:15004021..15006614. Its molecular function is described as acyltransferase activity. It is involved in the biological process microtubule-based movement. 11 alleles are reported. The phenotypes of these alleles are annotated with: macrochaeta; wing. It has 2 annotated transcripts and 2 annotated polypeptides.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
l(2)35Bf
Escapers show thin, short bristles and outstretched wings. l(2)35Bf3 partially complements l(2)35Bf1 and l(2)35Bf2 for viability; female-sterile alleles recovered by Lindsley.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
35B8-35B8  
Limits computationally determined from genome sequence between P{EP}elBEP2039&P{PZ}osprJ571 and P{lacW}Su(H)k07904  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
(Woodruff and Ashburner, 1979)
Right of (cM)
(Woodruff and Ashburner, 1979)
Notes
Excision of l(2)35Bck08808 indicates that the gene order is mol, dyn-p25, l(2)35Be, l(2)35Bc, l(2)35Bd.
(Ashburner et al., 1999.10.12)
Complementation analysis establishes the following gene order (distal to proximal): dyn-p25, l(2)35Be, l(2)35Bc.
(Ashburner et al., 1999)
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: CG31834+ dyn-p25+ CG4140-
(Ashburner et al., 1999)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\dyn-p25 for information on other features
detailed view FBtr0080694 FBtr0080693 FBtr0080728 FBtr0080695 FBpp0089266 FBpp0080255 FBpp0080287 FBpp0080256 FBti0050796 FBti0041011 FBti0068208
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
dyn-p25-RA
FBtr0080693
 NM_165097
  727
  189
dyn-p25-RB
FBtr0080694
 NM_135890
  618
  205
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
dyn-p25-PA  
FBpp0080255  
20.6  
189  
8.44  
  NP_723893
  AAF53426
dyn-p25-PB  
FBpp0089266  
22.6  
205  
9.17  
  NP_609734
  AAN10884
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Bacterial transferase hexapeptide repeat (IPR001451)
Trimeric LpxA-like (IPR011004)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AE003411
AAF44905
 
AE003646
AAF53426
 
AE003646
AAN10884
 
AF224265
AAF34709
 
AI386834
 
BG633017
 
CO152870
 
CO275427
 
CZ467742
 
EC072170
 
EC261745
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    (Ashburner et al., 1999.10.12)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive
        Sterility
        Allele
        female sterile
        Phenotype manifest in
        Allele
        macrochaeta
        wing
        hide Classical Alleles ( 10 )
        For All Classical Alleles Show

        Allele of dyn-p25ClassMutagenStocksKnown lesion
        dyn-p2510 --
        dyn-p2520 --
        dyn-p2530 --
        dyn-p2540 --
        dyn-p2551 --
        dyn-p2560 --
        dyn-p2570 --
        dyn-p2580 --
        dyn-p25QL530 --
        dyn-p25c021742 --
        hide Alleles Carried on Transgenic Constructs ( 1 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of dyn-p25ClassMutagenStocksKnown lesion
        dyn-p25GD34692 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Not disrupted in
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Roote, 1996.8)
        (Ashburner, 1982)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Ashburner, 1982, Gubb et al., 1984)
        (Iyengar et al., 1999, Gubb et al., 1984)
        (Ashburner, 1982, Gubb et al., 1984)
        (Furukawa et al., 1992)
        (Furukawa et al., 1992)
        (Furukawa et al., 1992)
        (Furukawa et al., 1992)
        (Furukawa et al., 1992)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Furukawa et al., 1992)
        (Woodruff and Ashburner, 1979)
        (O'Donnell et al., 1977, Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (O'Donnell et al., 1977, Woodruff and Ashburner, 1979, Ashburner et al., 1982, Ashburner, 1982)
        (O'Donnell et al., 1977, Ashburner et al., 1982)
        (O'Donnell et al., 1977, Woodruff and Ashburner, 1979, Ashburner et al., 1982, Ashburner, 1982)
        (Roote, 2000.6.13)
        (Furukawa et al., 1992)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        Disrupted in
        (O'Donnell et al., 1977, Woodruff and Ashburner, 1979, Ashburner, 1982, Furukawa et al., 1992)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Furukawa et al., 1992)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Ashburner, 1982)
        (Ashburner et al., 1982, Ashburner, 1982)
        (Ashburner et al., 1982, Ashburner, 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner, 1982, Furukawa et al., 1992)
        (Roote, 2004.6.24)
        (Ryder, 2004.4.26)
        (Ashburner, 1982)
        (Woodruff and Ashburner, 1979)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1984)
        (Gubb et al., 1984, Furukawa et al., 1992)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Ashburner, 1982, Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984, Furukawa et al., 1992)
        (Gubb et al., 1984)
        (Furukawa et al., 1992)
        (Furukawa et al., 1992)
        (Ashburner, 1982)
        (Furukawa et al., 1992)
        (O'Donnell et al., 1977, Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (O'Donnell et al., 1977, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (O'Donnell et al., 1977, Ashburner et al., 1982, Ashburner, 1982)
        (O'Donnell et al., 1977, Ashburner et al., 1982)
        (O'Donnell et al., 1977, Ashburner et al., 1982, Ashburner, 1982)
        (O'Donnell et al., 1977, Ashburner et al., 1982, Furukawa et al., 1992)
        (Furukawa et al., 1992)
        (Woodruff and Ashburner, 1979, Ashburner, 1982)
        (Ashburner and Harrington, 1984)
        (Ashburner and Harrington, 1984)
        (Gubb et al., 1985)
        (Ashburner and Harrington, 1984)
        (Ashburner and Harrington, 1984)
        (Woodruff and Ashburner, 1979)
        (Ashburner et al., 1982)
        (Ashburner and Harrington, 1984)
        (Ashburner and Harrington, 1984)
        Duplicated in
        (Ashburner, 1982)
        (Ashburner, 1982)
        (Ashburner, 1982)
        (Ashburner, 1982)
        (Ashburner, 1982)
        Not duplicated in
        (Ashburner, 1982)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD3469}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        miscellaneous insertions
        PBac{PB}dyn-p25c02174
        NA
        insertion of enhancer trap
        P{lacW}molSH1330
        No
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        hide Gene Ontology: Function, Process & Cellular Component ( 4 )
        hide Molecular Function
        CV term
        Evidence
        References
        acyltransferase activity
        inferred from electronic annotation with InterPro:IPR001451
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        microtubule-based movement
        inferred from sequence or structural similarity
        (Goldstein and Gunawardena, 2000)
        hide Cellular Component
        CV term
        Evidence
        References
        dynactin complex
        inferred from sequence or structural similarity
        (Goldstein and Gunawardena, 2000)
        non-traceable author statement
        (Eckley, 2000.1.12)
        microtubule associated complex
        inferred from electronic annotation
        (Roth and Foulger, 2002.4.24)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        dyn-p25 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 6 )
        Szeged
        -
        P{lacW}molSH1330
        VDRC
        v8057
        w1118; P{GD3469}v8057
        v8058
        w1118; P{GD3469}v8058
        Harvard
        -
        PBac{PB}dyn-p25c02174
        Bloomington
        3214
        b1 Adhn4 dyn-p255/CyO
        10853
        w1118; PBac{PB}dyn-p25c02174/CyO
        hide Genomic Clones ( 1 )
        hide cDNA Clones ( 3 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
        •
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for database merge of
        Source for merge of: dynactin-subunit-p25 BG:DS00929.1
        (Bayraktaroglu, 2000.8.7)
        Source for merge of: dyn-p25 l(2)35Bf
        (Roote, 2004.9.30)
        Additional comments
        "BG:DS00929.1" may correspond to "l(2)35Bf" or "l(2)35Be". "BG:DS00929.16", "BG:DS00929.1" or "BG:DS00929.2" may correspond to "l(2)35Bf".
        (Ashburner et al., 1999.10.12)
        hide Comments About Role
        hide Comments About Molecular Function
        hide Other Comments
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        RefSeq (Transcripts)
        •
        RefSeq (Proteins)
        •
        DDBJ / EMBL / GenBank
        •
        UniProtKB/TrEMBL
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Bacterial transferase hexapeptide repeat (IPR001451)
        Trimeric LpxA-like (IPR011004)
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
        •
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput tech