Gene Dmel\exo84

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General Information

Symbol

Dmel\exo84

Species

D. melanogaster

Name

exo84

Annotation symbol

CG6095

Feature type

protein_coding_gene

FlyBase ID

FBgn0086476

Created / Updated

2007-06-15/2007-06-15

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

96F10-96F10

Sequence location

3R:21,875,451..21,877,957 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol exo84 (CG6095, FBgn0086476). It has the cytological map location 96F10. Its sequence location is 3R:21875451..21877957. Its molecular function is unknown. It is involved in the biological processes: neurotransmitter secretion; synaptic vesicle docking during exocytosis; synaptic vesicle targeting; vesicle-mediated transport. 4 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96F10-96F10

Limits computationally determined from genome sequence between P{PZ}l(3)rQ197^rQ197 and P{lacW}scrib^j7B3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\exo84 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

exo84-RA

FBtr0084977

NM_143197

2223

671

exo84-RB

FBtr0084978

NM_206576

2226

672

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

exo84-PA

FBpp0084350

76.5

671

7.46

NP_651454

AAF56558

exo84-PB

FBpp0084351

76.7

672

7.64

NP_996299

AAS65219

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Pleckstrin-like (IPR001849)

Pleckstrin homology-type (IPR011993)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

RE36786

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of exo84	Class	Mutagen	Stocks	Known lesion
exo84^HP35566			1	--
exo84^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of exo84	Class	Mutagen	Stocks	Known lesion
exo84^GD11816		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
exo84^dsRNA.cSa		in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful duplication

Dp(3;3)M67C⁺5

Dp(3;3)M95A⁺13

Useful deficiency

Df(3R)Espl9

Disrupted in

Df(3R)Exel6204

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11816}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPg}exo84^HP35566

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

neurotransmitter secretion

non-traceable author statement

(Lloyd et al., 2000)

synaptic vesicle docking during exocytosis

non-traceable author statement

(Lloyd et al., 2000)

synaptic vesicle targeting

non-traceable author statement

(Lloyd et al., 2000)

vesicle-mediated transport

inferred from sequence or structural similarity

(Littleton, 2000)

Cellular Component

CV term

Evidence

References

exocyst

non-traceable author statement

(Lloyd et al., 2000)

inferred from sequence or structural similarity

(Littleton, 2000)

inferred from sequence or structural similarity with EMBL:AF032669

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

exo84 allele

Gene

References

External Data

Linkouts

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\GA19348

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

VDRC

v30111

w¹¹¹⁸; P{GD11816}v30111

v30112

w¹¹¹⁸; P{GD11816}v30112/TM3

Bloomington

21984

w¹¹¹⁸; P{EPg}exo84^HP35566

Genomic Clones ( 1 )

BACR13F13

cDNA Clones ( 9 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE36786

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG6095 anon-WO0153538.55 Source for merge of CG6095 anon-WO0153538.55 was sequence comparison (date:051113).

(FlyBase, 1996-)

Source for merge of: exo84 CG6095

(Sommer et al., 2005)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Pleckstrin-like (IPR001849)

Pleckstrin homology-type (IPR011993)

Linkouts

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/genebrief/exo84.htm

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

anon-WO0153538.55

CG6095

(Sommer et al., 2005, Stuart et al., 2007, Matthews, 2008.4.15)

DExo84

(Blankenship et al., 2006)

Exo84

(Sommer et al., 2005, Sommer et al., 2005, Littleton, 2000, Langevin et al., 2005)

exo84

(Lloyd et al., 2000, )

Exo84p

(Littleton, 2000)

Name Synonym

CG6095

exo84

Secondary FlyBase IDs

FBgn0042644
FBgn0039401
FBgn0045897

References ( 18 )

Research paper

Stuart et al., 2007, Nature 445(7123): 95--101: A systems biology analysis of the Drosophila phagosome. [FBrf0195342]
Langevin et al., 2005, Dev. Cell 9(3): 365--376: Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-cadherin trafficking from recycling endosomes to the plasma membrane. [FBrf0190210]
Sommer et al., 2005, J. Cell Biol. 169(6): 953--963: The exocyst component Sec5 is present on endocytic vesicles in the oocyte of Drosophila melanogaster. [FBrf0187825]
Harris et al., 2003, Genetics 165(2): 637--652: A deficiency screen of the major autosomes identifies a gene (matrimony) that is haplo-insufficient for achiasmate segregation in Drosophila oocytes. [FBrf0167662]
Murthy et al., 2003, Neuron 37(3): 433--447: Mutations in the exocyst component sec5 disrupt neuronal membrane traffic, but neurotransmitter release persists. [FBrf0156021]
Littleton, 2000, J. Cell Biol. 150(2): F77--F81: A genomic analysis of membrane trafficking and neurotransmitter release in Drosophila. [FBrf0129948]
Lloyd et al., 2000, Neuron 26(1): 45--50: A genome-wide search for synaptic vesicle cycle proteins in Drosophila. [FBrf0127207]

Supplementary material

Sommer et al., 2005, J. Cell Biol. 169(6):: Figure S1. Antisera and the yeast sec5 allele used in this study. [FBrf0199149]
Kiger et al., 2003, J. Biol. 2(4):: Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]

Personal communication to FlyBase

Matthews, 2008.4.15, DP and HP P-element insertions submitted to the Bloomington Drosophila Stock Center.: DP and HP P-element insertions submitted to the Bloomington Drosophila Stock Center. [FBrf0204789]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]

Abstract

Blankenship et al., 2006, A. Dros. Res. Conf. 47: 31: Function of DExo84 in maintenance of adherens junctions and apical identity in epithelial cell polarity. [FBrf0189471]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Patent

Greenspan and Edelman, 2001.7.26, World Patent 0153538-A:: Method for functional mapping of an alzheimer's disease gene network and for identifying therapeutic agents for the treatment of Alzheimer's disease. [FBrf0141237]

Curated genome annotation

Kodira, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126678]