Gene Dmel\Sra-1

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General Information

Symbol

Dmel\Sra-1

Species

D. melanogaster

Name

specifically Rac1-associated protein 1

Annotation symbol

CG4931

Feature type

protein_coding_gene

FlyBase ID

FBgn0038320

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

88F1-88F1

Sequence location

3R:11,159,001..11,164,103 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene specifically Rac1-associated protein 1 is referred to in FlyBase by the symbol Sra-1 (CG4931, FBgn0038320). It has the cytological map location 88F1. Its sequence location is 3R:11159001..11164103. Its molecular function is described as Rho GTPase binding. It is involved in the biological processes: axon guidance; regulation of synapse organization and biogenesis; cell projection biogenesis; cortical actin cytoskeleton organization and biogenesis; regulation of cell shape; phagocytosis, engulfment. 17 alleles are reported. The phenotypes of these alleles are annotated with: anterior fascicle; axon; longitudinal connective; commissure; synapse; bouton; embryonic central nervous system; eye; ommatidium; microchaeta. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88F1-88F1

Limits computationally determined from genome sequence between P{lacW}MRG15^j6A3 and P{PZ}l(3)06536⁰⁶⁵³⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

88E13-88F1

(Langmann, 2001.4.3)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Sra-1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Sra-1-RA

FBtr0083085

NM_142190

4631

1291

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Sra-1-PA

FBpp0082541

149.3

1291

7.13

NP_650447

AAF55173

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0038320

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Cytoplasmic FMR1-interacting (IPR008081)

Cytoplasmic FMR1-interacting, subgroup (IPR016536)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VF87

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-, Schenck, 2001.1.9)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

9499

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI546369

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0038320

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0038320

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable, with Scer\GAL4^elav-C155

Sra-1^Scer\UAS.cSa

semi-viable, with Scer\GAL4[-]:

Sra-1^EP3267

lethal | recessive

lethal | embryonic stage | germ-line clone

Sra-1^85.1

lethal | pupal stage | recessive

Sra-1^85.1

Other Phenotypes

Allele

cell shape defective

Sra-1^dsRNA.cKa

neuroanatomy defective | dominant

Sra-1^85.1

neuroanatomy defective, with Scer\GAL4^ey.PH

Sra-1^{dsRNA.Scer\UAS}

Sterility

Allele

fertile

Sra-1^EY06562

Phenotype manifest in

Allele

adult head & macrochaeta, with Scer\GAL4^sca.PC

trichogen cell & actin filament & pupa, with Scer\GAL4^sca.PC

anterior fascicle

Sra-1^85.1

axon

Sra-1^85.1

neuromuscular junction & synapse

Sra-1^85.1

longitudinal connective

Sra-1^85.1

commissure

Sra-1^85.1

synapse

Sra-1^85.1

bouton

Sra-1^85.1

embryonic central nervous system | germ-line clone

Sra-1^85.1

microchaeta, with Scer\GAL4^sca.PC

Sra-1^{Scer\UAS.T:Myr1}

photoreceptor cell & growth cone & larval brain, with Scer\GAL4^ey.PH

Sra-1^{dsRNA.Scer\UAS}

photoreceptor cell & stage 2 third instar larva, with Scer\GAL4^ey.PH

Sra-1^{dsRNA.Scer\UAS}

eye, with Scer\GAL4^ey.PH

Sra-1^{dsRNA.Scer\UAS}

ommatidium, with Scer\GAL4^ey.PH

Sra-1^{dsRNA.Scer\UAS}

larval neuromuscular junction & bouton, with Scer\GAL4^elav-C155

Sra-1^{dsRNA.Scer\UAS}

S2 cell-line & filopodium | ectopic

Sra-1^dsRNA.cBa

S2 cell-line & actin filament | ectopic

Sra-1^dsRNA.cBa

Classical Alleles ( 10 )

For All Classical Alleles Show

Allele of Sra-1	Class	Mutagen	Stocks	Known lesion
Sra-1^35.1	wild-type allele	P-element activity	0	Yes
Sra-1^5.2		P-element activity	0	--
Sra-1^70.1	wild-type allele	P-element activity	0	Yes
Sra-1^8.1		P-element activity	0	--
Sra-1^85.1		P-element activity	0	--
Sra-1^EP3267		P-element activity	1	--
Sra-1^EP789		P-element activity	1	--
Sra-1^EY06562		P-element activity	1	--
Sra-1^d02839		P-element activity	0	--
Sra-1^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Sra-1	Mutagen	Stocks	Known lesion
Sra-1^GD11477	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
Sra-1^{Scer\UAS.T:Myr1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
Sra-1^Scer\UAS.cSa	in vitro construct \| regulatory fusion	0	Yes
Sra-1^{dsRNA.Scer\UAS}	in vitro construct \| RNAi	0	Yes
Sra-1^dsRNA.cBa	in vitro construct \| RNAi	0	Yes
Sra-1^dsRNA.cKa	in vitro construct \| RNAi	0	Yes
Sra-1^{ΔC.Scer\UAS.T:Myr1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful duplication

Dp(3;3)C123.3

Disrupted in

Df(3R)Exel6174

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

characterization construct

UAS construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}Sra-1^EY06562

P{EP}Sra-1^EP3267

P{EP}Sra-1^EP789

P{XP}Sra-1^d02839

Related Comments

Please look at the allele reports for the complete phenotype data

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

Gene Ontology: Function, Process & Cellular Component ( 7 )

Molecular Function

CV term

Evidence

References

Rho GTPase binding

non-traceable author statement

(Langmann, 2001.4.3)

Biological Process

CV term

Evidence

References

axon guidance