A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Bruce

General Information
SymbolDmel\BruceSpeciesD. melanogaster
NameBruceAnnotation symbolCG6303
Feature typeprotein_coding_geneFlyBase IDFBgn0037808
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3RRecombination map
Cytogenetic map86A7-86A8Sequence location3R:6,138,366..6,157,071 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
This gene is referred to in FlyBase by the symbol Bruce (CG6303, FBgn0037808). It has the cytological map location 86A7-86A8. Its sequence location is 3R:6138366..6157071. Its molecular function is described as: small protein conjugating enzyme activity; ubiquitin-protein ligase activity. It is involved in the biological processes: anti-apoptosis; sperm individualization; programmed cell death; spermatid development; regulation of protein metabolic process; post-translational protein modification. 15 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
86A7-86A8  
Limits computationally determined from genome sequence between P{EP}Fmr1EP3517&P{PZ}tws02414 and P{PZ}jumu06439&P{lacW}l(3)j8B6j8B6  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Bruce for information on other features
detailed view FBtr0082240 FBtr0082239 FBtr0082230 FBtr0082231 FBpp0081718 FBpp0081717 FBpp0081708 FBpp0081709 FBti0011809 FBti0037012 FBti0021251 FBti0066578 FBti0041457 FBti0024476 FBti0039980
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Bruce-RA
FBtr0082239
 NM_141738
  14793
  4876
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Bruce-PA  
FBpp0081717  
539.2  
4876  
5.26  
  NP_649995
  AAF54520
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Ubiquitin-conjugating enzyme, E2 (IPR000608)
Proteinase inhibitor I32, inhibitor of apoptosis (IPR001370)
WD40 repeat-like (IPR011046)
Ubiquitin-conjugating enzyme/RWD-like (IPR016135)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA978573
 
AE003686
AAF54520
 
AF517634
AAM77747
 
AI113838
 
AI257027
 
AY119104
AAM50964
 
BF499746
 
BI167572
 
BI352722
 
BI353051
 
BI564273
 
BI638161
 
BI640753
 
BP547800
 
CK135263
 
CK604609
 
CO176986
 
CO263874
 
CO270294
 
CO293196
 
CO303497
 
CO317200
 
CO327620
 
CO340060
 
CO340142
 
CZ478812
 
CZ478813
 
EC054854
 
EC063720
 
EC066593
 
EC072294
 
EC081056
 
EC088595
 
EC212477
 
EC220147
 
EC223791
 
EC230124
 
EC252034
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Other Phenotypes
        Allele
        wild-type
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 12 )
        For All Classical Alleles Show

        Allele of BruceClassMutagenStocksKnown lesion
        BruceE120 Yes
        BruceE160 Yes
        BruceE81
          		0 Yes
          BruceGMREP-86a-10 Yes
          BruceGMREP-86a-20 Yes
          BruceGMREP-86a-30 Yes
          BruceGMREP-86a-40 Yes
          BruceGMREP-86a-50 Yes
          BruceGS3144
            		0 --
            Brucee009830 --
            Brucee009842 --
            Bruceunspecified
              		0 --
              hide Alleles Carried on Transgenic Constructs ( 3 )
              For All Alleles Carried on Transgenic Constructs Show

              Allele of BruceClassMutagenStocksKnown lesion
              BruceBIR.GMR0 Yes
              BruceGD169491 Yes
              BruceGMR.dsRNA0 Yes
              hide Aneuploid Aberrations
              Useful deficiency
               
              Useful duplication
               
              Disrupted in
              (Ryder, 2004.4.26)
              hide Transgenic Constructs & Insertions
              Transgenic Constructs
              Type of construct
              Name
              Expression data
              UAS construct
              P{GD16949}
              NA
              characterization construct
              P{GMR-Bruce.BIR}
              NA
              P{GMR-dBruce-RNAi}
              NA
              Insertions
              Type of insertions
              Name
              Expression data
              miscellaneous insertions
              PBac{RB}Brucee00983
              NA
              PBac{RB}Brucee00984
              NA
              P{GSV1}sleGS3144
              NA
              insertion of mobile activating element
              P{Mae-GMR.E}BruceGMREP-86a-1
              NA
              P{Mae-GMR.E}BruceGMREP-86a-2
              NA
              P{Mae-GMR.E}BruceGMREP-86a-3
              NA
              P{Mae-GMR.E}BruceGMREP-86a-4
              NA
              P{Mae-GMR.E}BruceGMREP-86a-5
              NA
              hide Related Comments
              Please look at the allele reports for the complete phenotype data
              hide Gene Ontology: Function, Process & Cellular Component ( 9 )
              hide Molecular Function
              CV term
              Evidence
              References
              small protein conjugating enzyme activity
              traceable author statement
              (Bergmann et al., 2003)
              ubiquitin-protein ligase activity
              inferred from sequence or structural similarity
              (Vernooy et al., 2000)
              inferred from sequence or structural similarity with EMBL:Y17267
              (FlyBase, 1992-)
              hide Biological Process
              CV term
              Evidence
              References
              anti-apoptosis
              inferred from sequence or structural similarity with MGD:Birc6; MGI:MGI:1276108
              (Vernooy et al., 2000)
              post-translational protein modification
              inferred from electronic annotation with InterPro:IPR000608
              (FlyBase Curators et al., 2004-)
              programmed cell death
              inferred from genetic interaction with FLYBASE:grim; FB:FBgn0015946 AND inferred from genetic interaction with FLYBASE:rpr; FB:FBgn0011706
              (Vernooy et al., 2002)
              regulation of protein metabolic process
              inferred from electronic annotation with InterPro:IPR000608
              (FlyBase Curators et al., 2004-)
              sperm individualization
              non-traceable author statement
              (Arama et al., 2002)
              spermatid development
              inferred from mutant phenotype
              (Arama et al., 2003)
              hide Cellular Component
              CV term
              Evidence
              References
              intracellular
              inferred from electronic annotation with InterPro:IPR001370
              (FlyBase Curators et al., 2004-)
              hide Sequence Ontology: Class of Gene
              protein_coding_gene
               
              nuclear_gene
               
              hide Interactions & Pathways
              hide Summary of Genetic Interactions
              Interacts with
              Please look at the allele data for full details of the genetic interactions
              Bruce allele
              Gene
              References
              BruceE12
              rpr
              (Vernooy et al., 2002)
              BruceE16
              rpr
              (Vernooy et al., 2002)
              BruceGMREP-86a-1
              rpr
              (Vernooy et al., 2002)
              BruceGMREP-86a-2
              rpr
              (Vernooy et al., 2002)
              Bruceunspecified
              rpr
              (Ryoo et al., 2002)
              unspecified
              rpr
              (Vernooy et al., 2002)
              hide External Data
              Linkouts
              BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
              •
              hide Orthologs
              Genome-wide drosophilid orthologs
              Curated drosophilid orthologs
              (Bartolome, 2005)
              (Bartolome, 2005)
              (Bartolome, 2005)
              Linkouts
              InParanoid orthologs - Eukaryotic orthologs
              •
              hide Functional Complementation between Species
              hide Inter-Species Misexpression Data
              Produces phenotype in
              Produces NO phenotype in
              hide Stocks & Reagents
              hide Stocks Listed in FlyBase ( 3 )
              Harvard
              -
              PBac{RB}Brucee00984
              VDRC
              v48309
              w1118; P{GD16949}v48309/CyO
              Bloomington
              17911
              w1118; PBac{RB}Brucee00984/TM6B, Tb1
              hide Genomic Clones ( 1 )
              hide cDNA Clones ( 21 )
              Please Note
              This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
              cDNA Clones, Fully Sequenced
              BDGP DGC clones
              Other clones
              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              hide RNAi & Array Information
              Affy Oligo
              Linkouts
              DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
              •
              GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
              •
              hide Antibody Information
              hide Other Information
              hide Discoverer
              hide Etymology
              hide Identification
              hide Position Effect Variegation Data
              hide Relationship to Other Genes
              Source for database identity of
              Source for identity of: Bruce CG6303
              (Vernooy et al., 2000)
              Source for identity of: Bruce CG6303
              (Vernooy et al., 2000)
              Source for database merge of
              Additional comments
              hide Comments About Role
              Bruce can inhibit cell death induced by grim and rpr but not W.
              (Vernooy et al., 2002)
              hide Comments About Molecular Function
              hide Other Comments
              dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
              (Kiger et al., 2003)
              hide External Crossreferences & Linkouts
              Sequence Crossreferences
              RefSeq (Transcripts)
              •
              RefSeq (Proteins)
              •
              DDBJ / EMBL / GenBank
              •
              UniProtKB/TrEMBL
              •
              Other Crossreferences
              InterPro domains - A database of protein families, domains, and functional sites
              •
              Ubiquitin-conjugating enzyme, E2 (IPR000608)
              Proteinase inhibitor I32, inhibitor of apoptosis (IPR001370)
              WD40 repeat-like (IPR011046)
              Ubiquitin-conjugating enzyme/RWD-like (IPR016135)
              Linkouts
              BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
              •
              DEDB - Drosophila exon database: splicing graphs
              •
              DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
              •
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              •
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              •
              FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
              •
              GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
              •
              GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
              •
              InParanoid orthologs - Eukaryotic orthologs
              •
              PANTHER - Protein classification by function, families, and pathways
              •
              hide Synonyms & Secondary IDs ( 6 )
              Reported As
              Symbol Synonym
              bruce
              (Ryoo et al., 2002, Ryoo et al., 2002)
              Bruce
              (Laviolette et al., 2005, Bartolome and Charlesworth, 2006)
              CG6303
              (Vernooy et al., 2000, Vernooy et al., 2000, Laviolette et al., 2005)
              dBruce
              (Orihara-Ono et al., 2005, Arama et al., 2003, Bergmann et al., 2003, Arama et al., 2001, Baehrecke, 2003)
              dbruce
              (Arama et al., 2002)
              dBRUCE
              (Vernooy et al., 2000, Vernooy et al., 2000)
              Name Synonym
              Bruce
               
              Secondary FlyBase IDs
                hide References ( 26 )
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                hide Recent research papers ( 1 )
                Bartolome and Charlesworth, 2006, Genetics 173(2): 779--791
                Rates and patterns of chromosomal evolution in Drosophila pseudoobscura and D. miranda. [FBrf0193909]
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                All reviews listed in FlyBase were published before 2006
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