Gene Dmel\Ada2b

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General Information

Symbol

Dmel\Ada2b

Species

D. melanogaster

Name

Ada2b

Annotation symbol

CG9638

Feature type

protein_coding_gene

FlyBase ID

FBgn0037555

Created / Updated

2005-03-17/2005-03-17

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

84F5-84F5

Sequence location

3R:4,062,838..4,066,234 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Ada2b (CG9638, FBgn0037555). It has the cytological map location 84F5. Its sequence location is 3R:4062838..4066234. Its molecular function is described as: histone acetyltransferase activity; protein binding; DNA binding; zinc ion binding. It is involved in the biological processes: positive regulation of histone acetylation; chromatin remodeling; regulation of transcription; axon target recognition. 7 alleles are reported. The phenotypes of these alleles are annotated with: dorsal mesothoracic disc; glial cell. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84F5-84F5

Limits computationally determined from genome sequence between P{PZ}grn⁰⁵⁹³⁰ and P{PZ}Mcm2^rL074

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: CG9636+ Ada2b+ DNApol-ι-

(Qi et al., 2004)

Gene Model & Products

Please see the GBrowse view of Dmel\Ada2b for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Ada2b-RA

FBtr0081807

NM_141516

2149

418

Ada2b-RB

FBtr0100368

NM_001031980

2073

555

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Ada2b-PA

FBpp0081303

48.3

418

9.02

NP_649773

AAF54199

Ada2b-PB

FBpp0099776

62.0

555

7.09

NP_001027151

AAZ52519

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0037555

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Zinc finger, ZZ-type (IPR000433)

SANT, DNA-binding (IPR001005)

Homeodomain-like (IPR009057)

Homeodomain-related (IPR012287)

Myb, DNA-binding (IPR014778)

Transcriptional adaptor 2 (IPR016827)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8I8V0

Maps to

Does NOT map to

Identified with

LD24527

(BDGP Project Members, 2000-, Kusch et al., 2003, Qi et al., 2004)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

Ada2b[1]
3R:4,065,241..4,066,317

comment=Imprecise excision of the P{EP} element, resulting in a 1077bp deletion that encompasses the Ada2b transcription start site and entire first exon.
evidence=experimental

(Qi et al., 2004)

deletion

Ada2b[2]
3R:4,063,541..4,066,317

comment=Imprecise excision of the P{EP} element, resulting in a 2.77kb deletion that removes almost the entire Ada2b gene.
evidence=experimental

(Qi et al., 2004)

rescue fragment

Ada2b[+t6.1]
3R:4,061,317..4,067,503

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental

(Qi et al., 2004)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

8615

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0037555

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0037555

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0037555

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pupal stage | recessive

Other Phenotypes

Allele

neuroanatomy defective

Ada2b¹

Sterility

Allele

female sterile | germ-line clone | recessive

Phenotype manifest in

Allele

axon & photoreceptor cell R2

Ada2b¹

axon & photoreceptor cell R3

Ada2b¹

axon & photoreceptor cell R4

Ada2b¹

axon & photoreceptor cell R5

Ada2b¹

glial cell

Ada2b¹

dorsal mesothoracic disc | conditional qualifier

Ada2b^unspecified

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of Ada2b	Mutagen	Stocks	Known lesion
Ada2b¹	P-element activity	0	Yes
Ada2b²	P-element activity	0	Yes
Ada2b^DG09704		1	--
Ada2b^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Ada2b	Mutagen	Stocks	Known lesion
Ada2b^+t6.1	in vitro construct \| genomic fragment	0	Yes
Ada2b^GD14276	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Ada2b^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)CA3

Useful duplication

Dp(3;3)D1

Disrupted in

Df(3R)dsx5

(Qi et al., 2004)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14276}

P{UAS-Ada2b.HAFL2}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{wHy}Ada2b^DG09704

insertion of mobile activating element

P{EP}EP3412

Related Comments

Please look at the allele reports for the complete phenotype data

Loss of Ada2b function results in segmentation defects as well as massive apoptosis at the onset of early embryonic differentiation.

(Kusch et al., 2003)

Ada2b mutant animals die as pupae without any gross morphological abnormalities. However, Ada2b is maternally contributed to the early embryo, which may mask an earlier phenotype.

(Dai and Mannervik, 2003)

Mutants show pupal lethality.

(Pankotai et al., 2003)

Mutants show a reduction in acetylation of His3 protein. Mutants show excessive p53-dependent apoptosis in response to gamma radiation.

(Qi et al., 2004)

Gene Ontology: Function, Process & Cellular Component ( 11 )

Molecular Function

CV term

Evidence

References

DNA binding

inferred from electronic annotation with InterPro:IPR001005, InterPro:IPR012287

(FlyBase Curators et al., 2004-)

histone acetyltransferase activity

non-traceable author statement

(Kusch, 2002.8.16)

protein binding

inferred from physical interaction with UniProtKB:Q9VWZ1 inferred from physical interaction with UniProtKB:O76216

(FBrf0198894)

zinc ion binding

inferred from electronic annotation with InterPro:IPR000433

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

axon target recognition

inferred from mutant phenotype

(Weake et al., 2008)

chromatin remodeling

inferred from direct assay

(FBrf0198894)

positive regulation of histone acetylation

inferred from mutant phenotype

(Qi et al., 2004)

regulation of transcription

inferred from direct assay

(Kusch et al., 2003)

Cellular Component

CV term

Evidence

References

SAGA complex

non-traceable author statement

(Kusch, 2002.8.16)

inferred from physical interaction with FLYBASE:Nipped-A; FB:FBgn0053554 AND inferred from physical interaction with FLYBASE:Spt3; FB:FBgn0037981

(Kusch et al., 2003)

nucleus

inferred from direct assay

(FBrf0198894)

polytene chromosome

inferred from direct assay

(Muratoglu et al., 2003)

inferred from direct assay

(Kusch et al., 2003)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Ada2b allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66149

Orthologs

Genome-wide drosophilid orthologs

Dana\GF16168

Dere\GG17470

Dgri\GH19246

Dmoj\GI24281

Dper\GL23274

Dpse\GA21933

Dsec\GM26364

Dsim\GD20886

Dvir\GJ23060

Dwil\GK14452

Dyak\GE24869

Curated drosophilid orthologs

Dpse\GA21933

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000020326
Anopheles gambiae (Ensembl)
ENSBTAP00000019478
Bos taurus (Ensembl)
ENSCAFP00000021182
Canis familiaris (Ensembl)
ENSCINP00000003889
Ciona intestinalis (Ensembl)
NEWSINFRUP00000131067
Takifugu rubripes (Ensembl)
ENSGALP00000025015
Gallus gallus (Ensembl)
ENSP00000308022
Homo sapiens (Ensembl)
ENSMMUP00000022368
Macaca mulatta (Ensembl)
ENSMODP00000003577
Monodelphis domestica (Ensembl)
ENSPTRP00000027374
Pan troglodytes (Ensembl)
ENSRNOP00000037910
Rattus norvegicus (Ensembl)
GSTENP00032876001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000049524
Xenopus tropicalis (Ensembl)
AT4G16420.1
Arabidopsis thaliana (Arabidopsis)
ZDB-CDNA-050522-557
Danio rerio (Zfin)
ZDB-GENE-050522-557
Danio rerio (Zfin)
DDB0216344
Dictyostelium discoideum (Dictybase)
YDR448W
Saccharomyces cerevisiae (Yeastgenome)
SPCC24B10.08c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Szeged

P{EP}EP3412

Bloomington

17125

w¹¹¹⁸; P{EP}EP3412

21472

y¹ w^67c23; P{wHy}Ada2b^DG09704

VDRC

v24076

w¹¹¹⁸; P{GD14276}v24076

Genomic Clones ( 1 )

BACR45A07

cDNA Clones ( 36 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones