Gene Dmel\Dcp2

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General Information

Symbol

Dmel\Dcp2

Species

D. melanogaster

Name

Decapping protein 2

Annotation symbol

CG6169

Feature type

protein_coding_gene

FlyBase ID

FBgn0036534

Created / Updated

2005-01-19/2007-06-17

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

72A1-72A1

Sequence location

3L:15,814,087..15,819,519 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Decapping protein 2 is referred to in FlyBase by the symbol Dcp2 (CG6169, FBgn0036534). It has the cytological map location 72A1. Its sequence location is 3L:15814087..15819519. Its molecular function is described as hydrolase activity. It is involved in the biological processes: mRNA catabolic process, nonsense-mediated decay; gene silencing by miRNA. 5 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

72A1-72A1

Limits computationally determined from genome sequence between P{EP}Tfb2^EP572 and P{lacW}th^j5C8&P{PZ}Mbs⁰³⁸⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Dcp2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Dcp2-RA

FBtr0075538

NM_140548

3674

791

Dcp2-RB

FBtr0075539

NM_168634

2844

564

Dcp2-RC

FBtr0075540

NM_206378

2783

774

Dcp2-RD

FBtr0100528

NM_001014585

2762

564

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Dcp2-PA

FBpp0075293

87.5

791

10.11

NP_648805

AAF49579

Dcp2-PB

FBpp0075294

63.3

564

10.10

NP_730074

AAN11769

Dcp2-PC

FBpp0075295

86.3

774

10.23

NP_996100

AAS64991

Dcp2-PD

FBpp0099969

63.3

564

10.10

NP_001014585

AAX52744

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0036534

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

NUDIX hydrolase, core (IPR000086)

Dcp2, box A (IPR007722)

NUDIX (IPR015797)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(Oliver, 2001.8.16)

(BDGP Project Members, 2000-)

LD07107

(Toba et al., 1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3987

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI107445

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0036534

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0036534

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0036534

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^dpp.blk1

Dcp2^GS3219

lethal, with Scer\GAL4^c355

Dcp2^GS3219

lethal, with Scer\GAL4^hs.2sev

Dcp2^GS3219

lethal, with Scer\GAL4^29BD

Dcp2^GS3219

Phenotype manifest in

Allele

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of Dcp2	Mutagen	Stocks	Known lesion
Dcp2^GS3219	Δ2-3	1	Yes
Dcp2^c02419	piggyBac transposase	1	--
Dcp2^e00034	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Dcp2	Class	Mutagen	Stocks	Known lesion
Dcp2^GD11844		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Dcp2^dsRNA.cMa		in vitro construct \| RNAi	0	--

Aneuploid Aberrations

Useful deficiency

Df(3L)th^SS102

Useful duplication

Dp(3;3)M71

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11844}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{GSV1}Dcp2^GS3219

miscellaneous insertions

PBac{PB}Dcp2^c02419

PBac{RB}Dcp2^e00034

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

hydrolase activity

inferred from electronic annotation with InterPro:IPR000086, InterPro:IPR015797

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

gene silencing by miRNA

inferred from mutant phenotype

(Rehwinkel et al., 2005)

NOT
mRNA catabolic process, nonsense-mediated decay

inferred from mutant phenotype

(Rehwinkel et al., 2005)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Dcp2 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65036

Orthologs

Genome-wide drosophilid orthologs

Dpse\GA19405

Dana\GF10373

Dere\GG13519

Dgri\GH14473

Dmoj\GI13619

Dper\GL24929

Dsec\GM24463

Dsim\GD12535

Dvir\GJ13955

Dwil\GK20641

Dyak\GE22821

Curated drosophilid orthologs

Dpse\GA19405

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

MGI:1917890
Mus musculus (Informatics)
DDB0185453
Dictyostelium discoideum (Dictybase)
ZDB-GENE-040426-851
Danio rerio (Zfin)
ZDB-CDNA-040425-3471
Danio rerio (Zfin)
ZDB-CDNA-040425-1779
Danio rerio (Zfin)
WBGene00003582
Caenorhabditis elegans (Wormbase)
AT5G13570.1
Arabidopsis thaliana (Arabidopsis)
Q6K837
Oryza sativa (Gramene)
ENSXETP00000046507
Xenopus tropicalis (Ensembl)
GSTENP00007196001
Tetraodon nigroviridis (Ensembl)
ENSRNOP00000015645
Rattus norvegicus (Ensembl)
ENSPTRP00000029331
Pan troglodytes (Ensembl)
ENSMODP00000019514
Monodelphis domestica (Ensembl)
ENSMMUP00000017826
Macaca mulatta (Ensembl)
ENSP00000329359
Homo sapiens (Ensembl)
ENSGALP00000000282
Gallus gallus (Ensembl)
NEWSINFRUP00000132066
Takifugu rubripes (Ensembl)
ENSCINP00000013467
Ciona intestinalis (Ensembl)
ENSCAFP00000023925
Canis familiaris (Ensembl)
ENSBTAP00000001015
Bos taurus (Ensembl)
ENSANGP00000012687
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

200560

VDRC

v22272

w¹¹¹⁸ P{GD11844}v22272

Genomic Clones ( 1 )

BACR06P13

cDNA Clones ( 23 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0036534

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0036534

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: Dcp2 CG6169

(Rehwinkel et al., 2005)

Source for database merge of

Source for merge of: CG6169 BEST:LD07107

(Rana, 2004.7.8)

Additional comments

Comments About Role

In contrast to the decapping enzymes in yeast and humans, the Dcp2 product does not seem to participate in the nonsense-mediated decay pathway.

(Rehwinkel et al., 2005)

Comments About Molecular Function

Other Comments

Area matching Drosophila ESTs AI107445 and AA390813.

(Davies, 2001.3.15)

Area matching Drosophila ESTs AI107445 and AA390813.

(Davies, 2001.3.15)

dsRNA has been made from templates generated with primers directed against this gene.

(Rehwinkel et al., 2005)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

NUDIX hydrolase, core (IPR000086)

Dcp2, box A (IPR007722)

NUDIX (IPR015797)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

65036

DEDB - Drosophila exon database: splicing graphs

•

3987

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0036534

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0036534

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0036534

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0036534

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0036534

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0036534

InParanoid orthologs - Eukaryotic orthologs

•

MGI:1917890
Mus musculus (Informatics)
DDB0185453
Dictyostelium discoideum (Dictybase)
ZDB-GENE-040426-851
Danio rerio (Zfin)
ZDB-CDNA-040425-3471
Danio rerio (Zfin)
ZDB-CDNA-040425-1779
Danio rerio (Zfin)
WBGene00003582
Caenorhabditis elegans (Wormbase)
AT5G13570.1
Arabidopsis thaliana (Arabidopsis)
Q6K837
Oryza sativa (Gramene)
ENSXETP00000046507
Xenopus tropicalis (Ensembl)
GSTENP00007196001
Tetraodon nigroviridis (Ensembl)
ENSRNOP00000015645
Rattus norvegicus (Ensembl)
ENSPTRP00000029331
Pan troglodytes (Ensembl)
ENSMODP00000019514
Monodelphis domestica (Ensembl)
ENSMMUP00000017826
Macaca mulatta (Ensembl)
ENSP00000329359
Homo sapiens (Ensembl)
ENSGALP00000000282
Gallus gallus (Ensembl)
NEWSINFRUP00000132066
Takifugu rubripes (Ensembl)
ENSCINP00000013467
Ciona intestinalis (Ensembl)
ENSCAFP00000023925
Canis familiaris (Ensembl)
ENSBTAP00000001015
Bos taurus (Ensembl)
ENSANGP00000012687
Anopheles gambiae (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0036534

University of Chicago Dev. Expression

•

FBgn0036534

Synonyms & Secondary IDs ( 8 )

Reported As

Symbol Synonym

BEST:LD07107

CG6169

(Ma et al., 2006)

Dcp2

DCP2

(Rehwinkel et al., 2005)

DCp2p

(Valencia-Sanchez et al., 2006)

dDcp2

(Seydoux and Braun, 2006)

LD07107

(Toba et al., 1999)

Name Synonym

CG6169

Decapping protein 2

Secondary FlyBase IDs

FBgn0026583

References ( 17 )

Research paper

Bjorklund et al., 2006, Nature 439(7079): 1009--1013: Identification of pathways regulating cell size and cell-cycle progression by RNAi. [FBrf0191233]
Ma et al., 2006, Nature 443(7109): 359--363: Prevalence of off-target effects in Drosophila RNA interference screens. [FBrf0194302]
Rehwinkel et al., 2005, RNA, N.Y. 11(11): 1640--1647: A crucial role for GW182 and