Gene Dmel\sec63

Help

Close All Open All

General Information

Symbol

Dmel\sec63

Species

D. melanogaster

Name

sec63

Annotation symbol

CG8583

Feature type

protein_coding_gene

FlyBase ID

FBgn0035771

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

65F7-65F7

Sequence location

3L:7,353,663..7,357,466 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol sec63 (CG8583, FBgn0035771). It has the cytological map location 65F7. Its sequence location is 3L:7353663..7357466. Its molecular function is described as: signal recognition particle binding; heat shock protein binding; unfolded protein binding. It is involved in the biological process protein folding. 11 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65F7-65F7

Limits computationally determined from genome sequence between P{EP}lark^EP3259&P{lacW}l(3)j1D5^j1D5 and P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\sec63 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

sec63-RA

FBtr0076847

NM_139854

2942

753

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

sec63-PA

FBpp0076558

85.9

753

5.82

NP_648111

AAF50571

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0035771

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Heat shock protein DnaJ, N-terminal (IPR001623)

Heat shock protein DnaJ (IPR003095)

Sec63 (IPR004179)

Molecular chaperone, heat shock protein, Hsp40, DnaJ (IPR015609)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VS57

Maps to

Does NOT map to

Identified with

RE14391

(BDGP Project Members, 2000-, Abrams and Andrew, 2005)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

4959

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI455815
AA941759

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035771

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0035771

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0035771

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG8583

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for sec63

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Phenotype manifest in

Allele

Classical Alleles ( 10 )

For All Classical Alleles Show

Allele of sec63	Mutagen	Stocks	Known lesion
sec63^CA06603	P-element activity	0	Yes
sec63^CB04752	P-element activity	0	Yes
sec63^EY04730	P-element activity	1	--
sec63^GS3104		0	Yes
sec63^KG01781		0	--
sec63^KG09480		0	--
sec63^d00726	P-element activity	0	--
sec63^d08201	P-element activity	0	--
sec63^e03550	piggyBac transposase	1	--
sec63^f01027	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of sec63	Class	Mutagen	Stocks	Known lesion
sec63^GD2348		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)pbl-X1

Useful duplication

Dp(3;3)M67C⁺1

Disrupted in

Df(3L)Exel8104

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD2348}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}sec63^e03550

PBac{WH}sec63^f01027

P{PTT-GA}sec63^CA06603

P{PTT-GB}sec63^CB04752

P{SUPor-P}sec63^KG01781

P{SUPor-P}sec63^KG09480

insertion of mobile activating element

P{EPgy2}sec63^EY04730

P{GSV1}sec63^GS3104

P{XP}sec63^d00726

P{XP}sec63^d08201

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

heat shock protein binding

inferred from electronic annotation with InterPro:IPR001623, InterPro:IPR003095

(FlyBase Curators et al., 2004-)

signal recognition particle binding

inferred from sequence or structural similarity with EMBL:AF100141

(FlyBase, 1992-)

unfolded protein binding

inferred from electronic annotation with InterPro:IPR003095

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

protein folding

inferred from electronic annotation with InterPro:IPR003095

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

signal recognition particle receptor complex

inferred from sequence or structural similarity with EMBL:AF100141

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

sec63 allele

Gene

References

unspecified

pbl

(Gregory et al., 2007)

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

64259

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521926

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10637

Dere\GG14419

Dgri\GH14875

Dmoj\GI12848

Dper\GL26342

Dpse\GA21179

Dsec\GM14835

Dsim\GD14009

Dvir\GJ12993

Dwil\GK13558

Dyak\GE21609

Curated drosophilid orthologs

Dpse\GA21179

Dyak\GE21609

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000017941
Anopheles gambiae (Ensembl)
ENSBTAP00000022030
Bos taurus (Ensembl)
ENSCAFP00000005595
Canis familiaris (Ensembl)
ENSCINP00000006134
Ciona intestinalis (Ensembl)
NEWSINFRUP00000170006
Takifugu rubripes (Ensembl)
ENSGALP00000024664
Gallus gallus (Ensembl)
ENSP00000027474
Homo sapiens (Ensembl)
ENSMMUP00000021818
Macaca mulatta (Ensembl)
ENSMODP00000022461
Monodelphis domestica (Ensembl)
ENSPTRP00000031547
Pan troglodytes (Ensembl)
ENSRNOP00000000345
Rattus norvegicus (Ensembl)
GSTENP00030258001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000032524
Xenopus tropicalis (Ensembl)
Q7XVN7
Oryza sativa (Gramene)
AT4G21180.1
Arabidopsis thaliana (Arabidopsis)
WBGene00038167
Caenorhabditis briggsae (Wormbase)
WBGene00001047
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040718-361
Danio rerio (Zfin)
ZDB-GENE-040718-328
Danio rerio (Zfin)
DDB0186819
Dictyostelium discoideum (Dictybase)
MGI:2155302
Mus musculus (Informatics)
SPBC36B7.03
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

v33281

w¹¹¹⁸; P{GD2348}v33281/TM3

v33282

w¹¹¹⁸; P{GD2348}v33282/TM3

Bloomington

14397

y¹; P{SUPor-P}KG01781 ry⁵⁰⁶

14787

y¹ w^67c23; P{SUPor-P}KG09480 ry⁵⁰⁶

15766

y¹ w^67c23; P{EPgy2}sec63^EY04730/TM3, Sb¹ Ser¹

Genomic Clones ( 2 )

cDNA Clones ( 63 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones