Gene Dmel\CG12082

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General Information

Symbol

Dmel\CG12082

Species

D. melanogaster

Name

CG12082

Annotation symbol

CG12082

Feature type

protein_coding_gene

FlyBase ID

FBgn0035402

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

63B6-63B7

Sequence location

3L:3,145,405..3,148,358 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG12082 (FBgn0035402). It has the cytological map location 63B6-63B7. Its sequence location is 3L:3145405..3148358. Its molecular function is described as: ubiquitin-specific protease 5 activity; ubiquitin thiolesterase activity; zinc ion binding. It is involved in the biological processes: protein deubiquitination; ubiquitin-dependent protein catabolic process. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63B6-63B7

Limits computationally determined from genome sequence between P{PZ}l(3)06803⁰⁶⁸⁰³ and P{PZ}kst⁰¹³¹⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CG12082 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG12082-RA

FBtr0073114

NM_139516

2779

827

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG12082-PA

FBpp0072976

92.1

827

5.16

NP_647773

AAF47720

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0035402

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-associated/translation elongation factor EF1B, N-terminal (IPR000449)

Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394)

Zinc finger, UBP-type (IPR001607)

UBA-like (IPR009060)

Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote (IPR015940)

Ubiquitinyl hydrolase (IPR016652)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VZU7

Maps to

Does NOT map to

Identified with

RE70722

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2166

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI512423

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035402

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0035402

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0035402

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG12082

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for CG12082

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of CG12082	Class	Mutagen	Stocks	Known lesion
CG12082^EY20760		P-element activity	1	--
CG12082^EY23569		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG12082	Class	Mutagen	Stocks	Known lesion
CG12082^GD6741		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)M21

Useful duplication

Dp(3;3)SMG38

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD6741}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}CG12082^EY20760

P{EPgy2}CG12082^EY23569

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

ubiquitin thiolesterase activity

inferred from electronic annotation with InterPro:IPR001394

(FlyBase Curators et al., 2004-)

ubiquitin-specific protease 5 activity

traceable author statement

(MEROPS Project Members, 1996-)

zinc ion binding

inferred from electronic annotation with InterPro:IPR001607

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

protein deubiquitination

traceable author statement

(MEROPS Project Members, 1996-)

ubiquitin-dependent protein catabolic process

inferred from electronic annotation with InterPro:IPR001394

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG12082 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63873

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522328

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10220

Dere\GG14479

Dgri\GH15672

Dmoj\GI12716

Dper\GL20751

Dpse\GA11383

Dsec\GM14081

Dsim\GD13355

Dvir\GJ12705

Dwil\GK18032

Dyak\GE21667

Curated drosophilid orthologs

Dpse\GA11383

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009085
Anopheles gambiae (Ensembl)
ENSBTAP00000026356
Bos taurus (Ensembl)
ENSCAFP00000021591
Canis familiaris (Ensembl)
ENSCINP00000014354
Ciona intestinalis (Ensembl)
NEWSINFRUP00000175670
Takifugu rubripes (Ensembl)
ENSGALP00000023395
Gallus gallus (Ensembl)
ENSP00000229268
Homo sapiens (Ensembl)
ENSMMUP00000006871
Macaca mulatta (Ensembl)
ENSMODP00000022630
Monodelphis domestica (Ensembl)
ENSPTRP00000026932
Pan troglodytes (Ensembl)
ENSRNOP00000020785
Rattus norvegicus (Ensembl)
GSTENP00028927001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000051604
Xenopus tropicalis (Ensembl)
Q5VRV7
Oryza sativa (Gramene)
AT3G20630.1
Arabidopsis thaliana (Arabidopsis)
WBGene00039306
Caenorhabditis briggsae (Wormbase)
WBGene00020839
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-3531
Danio rerio (Zfin)
ZDB-CDNA-050612-45
Danio rerio (Zfin)
ZDB-GENE-040426-2584
Danio rerio (Zfin)
DDB0231492
Dictyostelium discoideum (Dictybase)
MGI:1347343
Mus musculus (Informatics)
YBR058C
Saccharomyces cerevisiae (Yeastgenome)
SPBC6B1.06c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v17567

w¹¹¹⁸; P{GD6741}v17567

v17568

w¹¹¹⁸; P{GD6741}v17568

Bloomington

22644

y¹ w^67c23; P{EPgy2}CG12082^EY23569

22426

y¹ w^67c23; P{EPgy2}CG12082^EY20760/TM3, Sb¹ Ser¹

Genomic Clones ( 1 )

BACR48E19

cDNA Clones ( 13 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE70722

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0035402

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0035402

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_139516

RefSeq (Proteins)

•

NP_647773

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q9VZU7

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ubiquitin-associated/translation elongation factor EF1B, N-terminal (IPR000449)

Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394)

Zinc finger, UBP-type (IPR001607)

UBA-like (IPR009060)

Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote (IPR015940)

Ubiquitinyl hydrolase (IPR016652)

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for CG12082

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63873

DEDB - Drosophila exon database: splicing graphs

•

2166

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0035402

Drosophila PIMRider - The Drosophila Protein Interaction map

•

522328

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035402

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0035402

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0035402

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0035402

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0035402

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0035402

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000009085
Anopheles gambiae (Ensembl)
ENSBTAP00000026356
Bos taurus (Ensembl)
ENSCAFP00000021591
Canis familiaris (Ensembl)
ENSCINP00000014354
Ciona intestinalis (Ensembl)
NEWSINFRUP00000175670
Takifugu rubripes (Ensembl)
ENSGALP00000023395
Gallus gallus (Ensembl)
ENSP00000229268
Homo sapiens (Ensembl)
ENSMMUP00000006871
Macaca mulatta (Ensembl)
ENSMODP00000022630
Monodelphis domestica (Ensembl)
ENSPTRP00000026932
Pan troglodytes (Ensembl)
ENSRNOP00000020785
Rattus norvegicus (Ensembl)
GSTENP00028927001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000051604
Xenopus tropicalis (Ensembl)
Q5VRV7
Oryza sativa (Gramene)
AT3G20630.1
Arabidopsis thaliana (Arabidopsis)
WBGene00039306
Caenorhabditis briggsae (Wormbase)
WBGene00020839
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-3531
Danio rerio (Zfin)
ZDB-CDNA-050612-45
Danio rerio (Zfin)
ZDB-GENE-040426-2584
Danio rerio (Zfin)
DDB0231492
Dictyostelium discoideum (Dictybase)
MGI:1347343
Mus musculus (Informatics)
YBR058C
Saccharomyces cerevisiae (Yeastgenome)
SPBC6B1.06c
Schizosaccharomyces pombe (Sanger)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0035402

University of Chicago Dev. Expression

•

FBgn0035402

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG12082

Name Synonym

CG12082

Secondary FlyBase IDs

References ( 10 )

Research paper

Chen and Fischer, 2000, Genetics 156(4): 1829--1836: In vivo structure/function analysis of the Drosophila fat facets deubiquitinating enzyme gene. [FBrf0131273]

Supplementary material

Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Bellen, 2006.8.3, MBBatch686_2.xml: MBBatch686_2.xml [FBrf0191805]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]
MEROPS Project Members, 1996-, MEROPS.: MEROPS. [FBrf0128212]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]

Curated genome annotation

Zhu, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126704]