Gene Dmel\mRpL23

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General Information

Symbol

Dmel\mRpL23

Species

D. melanogaster

Name

mitochondrial ribosomal protein L23

Annotation symbol

CG1320

Feature type

protein_coding_gene

FlyBase ID

FBgn0035335

Created / Updated

2003-12-01/2008-01-31

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

62D7-62D7

Sequence location

3L:2,372,841..2,373,678 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene mitochondrial ribosomal protein L23 is referred to in FlyBase by the symbol mRpL23 (CG1320, FBgn0035335). It has the cytological map location 62D7. Its sequence location is 3L:2372841..2373678. Its molecular function is described as: structural constituent of ribosome; nucleotide binding. It is involved in the biological process translation. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

62D7-62D7

Limits computationally determined from genome sequence between P{PZ}sls^rL182 and P{PZ}Spn⁰⁶⁹¹¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\mRpL23 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

mRpL23-RA

FBtr0072900

NM_079165

598

150

mRpL23-RB

FBtr0289966

625

150

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

mRpL23-PA

FBpp0072778

17.9

150

10.13

NP_523889

AAF47639

mRpL23-PB

FBpp0288404

17.9

150

10.13

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Nucleotide-binding, alpha-beta plait (IPR012677)

Ribosomal protein L23/L15e, core (IPR012678)

Ribosomal protein L25/L23 (IPR013025)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W021

Maps to

Does NOT map to

Identified with

RE49852

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2074

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035335

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0035335

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0035335

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for mRpL23

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for mRpL23

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of mRpL23	Class	Mutagen	Stocks	Known lesion
mRpL23^DG02505		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mRpL23	Class	Mutagen	Stocks	Known lesion
mRpL23^GD16486		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
mRpL23^GD7597		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)Aprt-32

Useful duplication

Dp(3;3)SMG38

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD16486}

P{GD7597}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{wHy}mRpL23^DG02505

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

nucleotide binding

inferred from electronic annotation with InterPro:IPR012677

(FlyBase Curators et al., 2004-)

structural constituent of ribosome

inferred from sequence or structural similarity with EMBL:AB049646

(Suzuki et al., 2001)

inferred from sequence or structural similarity with EMBL:U62635

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

translation

inferred from sequence or structural similarity with EMBL:AB049646

(Suzuki et al., 2001)

inferred from sequence or structural similarity with EMBL:U62635

(FlyBase, 1992-)

Cellular Component

CV term

Evidence

References

mitochondrial large ribosomal subunit

inferred from sequence or structural similarity with EMBL:AB049646

(Suzuki et al., 2001)

inferred from sequence or structural similarity with EMBL:U62635

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

mRpL23 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63817

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24868

Dere\GG14520

Dgri\GH15005

Dmoj\GI13167

Dper\GL24615

Dpse\GA12114

Dsec\GM14124

Dsim\GD13394

Dvir\GJ11942

Dwil\GK12514

Dyak\GE21711

Curated drosophilid orthologs

Dpse\GA12114

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000027440
Anopheles gambiae (Ensembl)
ENSBTAP00000036473
Bos taurus (Ensembl)
ENSCAFP00000014826
Canis familiaris (Ensembl)
ENSCINP00000005704
Ciona intestinalis (Ensembl)
NEWSINFRUP00000148519
Takifugu rubripes (Ensembl)
ENSGALP00000010578
Gallus gallus (Ensembl)
ENSMODP00000004204
Monodelphis domestica (Ensembl)
ENSRNOP00000027585
Rattus norvegicus (Ensembl)
GSTENP00019787001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000030600
Xenopus tropicalis (Ensembl)
WBGene00033494
Caenorhabditis briggsae (Wormbase)
WBGene00020348
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040625-18
Danio rerio (Zfin)
ZDB-GENE-040625-12
Danio rerio (Zfin)
MGI:1196612
Mus musculus (Informatics)
SPAC31A2.08
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v18101

w¹¹¹⁸; P{GD7597}v18101

v52256

w¹¹¹⁸; P{GD16486}v52256

v52257

w¹¹¹⁸; P{GD16486}v52257

Bloomington

22265

y¹ w^67c23; P{wHy}mRpL23^DG02505/TM3, Sb¹ Ser¹

Genomic Clones ( 2 )

cDNA Clones ( 7 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE49852

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE62129

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0035335

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0035335

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for identity of: mRpL23 CG1320

(Suzuki et al., 2001)

Source for database merge of

Source for merge of: mRpL23 BcDNA:RE49852 Source for merge of mRpL23 BcDNA:RE49852 was TrEMBL update (date:020807).

(FlyBase, 1996-)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079165

RefSeq (Proteins)

•

NP_523889

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q9W021

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Nucleotide-binding, alpha-beta plait (IPR012677)

Ribosomal protein L23/L15e, core (IPR012678)

Ribosomal protein L25/L23 (IPR013025)

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for mRpL23

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63817

DEDB - Drosophila exon database: splicing graphs

•

2074

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0035335

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0035335

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0035335

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0035335

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0035335

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0035335

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0035335

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000027440
Anopheles gambiae (Ensembl)
ENSBTAP00000036473
Bos taurus (Ensembl)
ENSCAFP00000014826
Canis familiaris (Ensembl)
ENSCINP00000005704
Ciona intestinalis (Ensembl)
NEWSINFRUP00000148519
Takifugu rubripes (Ensembl)
ENSGALP00000010578
Gallus gallus (Ensembl)
ENSMODP00000004204
Monodelphis domestica (Ensembl)
ENSRNOP00000027585
Rattus norvegicus (Ensembl)
GSTENP00019787001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000030600
Xenopus tropicalis (Ensembl)
WBGene00033494
Caenorhabditis briggsae (Wormbase)
WBGene00020348
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040625-18
Danio rerio (Zfin)
ZDB-GENE-040625-12
Danio rerio (Zfin)
MGI:1196612
Mus musculus (Informatics)
SPAC31A2.08
Schizosaccharomyces pombe (Sanger)

Synonyms & Secondary IDs ( 5 )

Reported As

Symbol Synonym

BcDNA:RE49852

CG1320

(Suzuki et al., 2001, )

L23

(Suzuki et al., 2001)

mRpL23

Name Synonym

mitochondrial ribosomal protein L23

(Suzuki et al., 2001)

Secondary FlyBase IDs

FBgn0047103

References ( 9 )

Research paper

Suzuki et al., 2001, J. Biol. Chem. 276(24): 21724--21736: Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria. [FBrf0136752]

Personal communication to FlyBase

BDGP TI submission, 2008.3.31, DG.xml: DG.xml [FBrf0204101]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Curated genome annotation

Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126651]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]