Gene Dmel\cpa

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General Information

Symbol

Dmel\cpa

Species

D. melanogaster

Name

capping protein alpha

Annotation symbol

CG10540

Feature type

protein_coding_gene

FlyBase ID

FBgn0034577

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

57B16-57B16

Sequence location

2R:16,945,296..16,947,388 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene capping protein alpha is referred to in FlyBase by the symbol cpa (CG10540, FBgn0034577). It has the cytological map location 57B16. Its sequence location is 2R:16945296..16947388. Its molecular function is described as actin binding. It is involved in the biological processes: actin filament organization; barbed-end actin filament capping; microtubule-based movement; actin cytoskeleton organization and biogenesis; negative regulation of actin filament polymerization; wing disc development; phagocytosis, engulfment. 12 alleles are reported. The phenotypes of these alleles are annotated with: eye; wing; macrochaeta; dorsal mesothoracic disc. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

57B16-57B16

Limits computationally determined from genome sequence between P{EP}grau^EP688&P{PZ}shg¹⁰⁴⁶⁹ and P{PZ}king-tubby⁰²⁸²¹&P{lacW}Xbp1^k13803

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\cpa for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

cpa-RA

FBtr0071532

NM_137695

1914

286

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

cpa-PA

FBpp0071461

32.8

286

5.80

NP_611539

AAF46660

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0034577

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

F-actin capping protein, alpha subunit (IPR002189)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W2N0

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

GH10050

(BDGP Project Members, 2000-, Janody and Treisman, 2006)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

1113

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI113652

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034577

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0034577

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0034577

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal (with cpa^scrd1)

cpa^scrd2

lethal (with cpa^scrd2)

cpa^scrd1

lethal (with cpa^43D)

lethal (with cpa^69E)

cpa^43D

lethal (with cpa^107E)

cpa^43D

lethal | recessive

cell lethal | partially | somatic clone

Other Phenotypes

Allele

visible | recessive | somatic clone

cpa^unspecified

visible | somatic clone

cpa^KG02261

Phenotype manifest in

Allele

eye | somatic clone

actin cytoskeleton & eye disc | somatic clone

cpa^unspecified

wing (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^69E

cpa^{Scer\UAS.T:Ivir\HA1}

eye (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^69E

cpa^{Scer\UAS.T:Ivir\HA1}

macrochaeta (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^69E

cpa^{Scer\UAS.T:Ivir\HA1}

wing (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^107E

cpa^{Scer\UAS.T:Ivir\HA1}

eye (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^107E

cpa^{Scer\UAS.T:Ivir\HA1}

macrochaeta (with cpa^43D), with Scer\GAL4^αTub84B.PL, cpa^107E

cpa^{Scer\UAS.T:Ivir\HA1}

wing (with cpa^69E), with Scer\GAL4^αTub84B.PL, cpa^43D

cpa^{Scer\UAS.T:Ivir\HA1}

eye (with cpa^69E), with Scer\GAL4^αTub84B.PL, cpa^43D

cpa^{Scer\UAS.T:Ivir\HA1}

macrochaeta (with cpa^69E), with Scer\GAL4^αTub84B.PL, cpa^43D

cpa^{Scer\UAS.T:Ivir\HA1}

wing (with cpa^107E), with Scer\GAL4^αTub84B.PL, cpa^43D

cpa^{Scer\UAS.T:Ivir\HA1}

eye (with cpa^107E), with Scer\GAL4^αTub84B.PL, cpa^43D

cpa^{Scer\UAS.T:Ivir\HA1}

macrochaeta (with cpa^107E), with Scer\GAL4^αTub84B.PL, cpa^43D

cpa^{Scer\UAS.T:Ivir\HA1}

dorsal mesothoracic disc | somatic clone

wing (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^69E

cpa^43D

eye (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^69E

cpa^43D

macrochaeta (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^69E

cpa^43D

wing (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^107E

cpa^43D

eye (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^107E

cpa^43D

macrochaeta (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^107E

cpa^43D

wing, with Scer\GAL4^αTub84B.PL, cpa^69E/cpa^{Scer\UAS.T:Ivir\HA1}

cpa^43D

eye, with Scer\GAL4^αTub84B.PL, cpa^69E/cpa^{Scer\UAS.T:Ivir\HA1}

cpa^43D

macrochaeta, with Scer\GAL4^αTub84B.PL, cpa^69E/cpa^{Scer\UAS.T:Ivir\HA1}

cpa^43D

wing, with Scer\GAL4^αTub84B.PL, cpa^{Scer\UAS.T:Ivir\HA1}/cpa^107E

cpa^43D

eye, with Scer\GAL4^αTub84B.PL, cpa^{Scer\UAS.T:Ivir\HA1}/cpa^107E

cpa^43D

macrochaeta, with Scer\GAL4^αTub84B.PL, cpa^{Scer\UAS.T:Ivir\HA1}/cpa^107E

cpa^43D

wing, with Scer\GAL4^αTub84B.PL, cpa^{Scer\UAS.T:Ivir\HA1}/cpa^43D

eye, with Scer\GAL4^αTub84B.PL, cpa^{Scer\UAS.T:Ivir\HA1}/cpa^43D

macrochaeta, with Scer\GAL4^αTub84B.PL, cpa^{Scer\UAS.T:Ivir\HA1}/cpa^43D

wing (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^43D

eye (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^43D

macrochaeta (with cpa^{Scer\UAS.T:Ivir\HA1}), with Scer\GAL4^αTub84B.PL, cpa^43D

Classical Alleles ( 10 )

For All Classical Alleles Show

Allele of cpa	Mutagen	Stocks	Known lesion
cpa^107E	ethyl methanesulfonate	0	Yes
cpa^36P	ethyl methanesulfonate	0	Yes
cpa^43D	ethyl methanesulfonate	0	Yes
cpa^66A	ethyl methanesulfonate	0	--
cpa^69E	ethyl methanesulfonate	0	Yes
cpa^KG02261	P-element activity	1	Yes
cpa^d10354	P-element activity	0	--
cpa^scrd1	ethyl methanesulfonate	0	--
cpa^scrd2	ethyl methanesulfonate	0	Yes
cpa^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of cpa	Class	Mutagen	Stocks	Known lesion
cpa^GD7009		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
cpa^{Scer\UAS.T:Ivir\HA1}			0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)PI13

Useful duplication

Dp(2;Y)53D;57C

Disrupted in

Df(2R)Exel6072

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7009}

P{UAS-cpa.HA}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}cpa^KG02261

insertion of mobile activating element

P{XP}cpa^d10354

Related Comments

Please look at the allele reports for the complete phenotype data

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: increased or polarized (uneven) accumulation of F-actin.

(Kiger et al., 2003)

Mutant clones in the imaginal discs accumulate actin filaments all around the cell cortex. Mutant clones in the eye disc cause photoreceptor nuclei to drop out of the eye disc epithelium and move towards and through the optic stalk. Mutant clones are not recovered in the epithelium of the wing pouch, although they can be recovered in the epithelium of the remainder of the wing disc.

(Janody et al., 2005)

Gene Ontology: Function, Process & Cellular Component ( 12 )

Molecular Function

CV term

Evidence

References

actin binding

inferred from electronic annotation with InterPro:IPR002189

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

actin cytoskeleton organization and biogenesis

inferred from mutant phenotype

(Delalle et al., 2005)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

actin filament organization

inferred from mutant phenotype

(Kiger et al., 2003)

barbed-end actin filament capping

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

inferred from sequence or structural similarity with UniProtKB:P13127

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

microtubule-based movement

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

negative regulation of actin filament polymerization

inferred from mutant phenotype

(Janody and Treisman, 2006)

phagocytosis, engulfment

inferred from mutant phenotype

(Stroschein-Stevenson et al., 2006)

wing disc development

inferred from mutant phenotype

(Janody and Treisman, 2006)

Cellular Component

CV term

Evidence

References

F-actin capping protein complex

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

inferred from electronic annotation with InterPro:IPR002189

(FlyBase Curators et al., 2004-)

apical plasma membrane

inferred from direct assay

(Janody and Treisman, 2006)

dynactin complex

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

microtubule associated complex

inferred from electronic annotation

(Roth and Foulger, 2002.4.24)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

cpa allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63026

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521419

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11927

Dere\GG22079

Dgri\GH20526

Dmoj\GI18973

Dper\GL10145

Dpse\GA10384

Dsec\GM15798

Dsim\GD11558

Dvir\GJ22434

Dwil\GK23266

Dyak\GE12160

Curated drosophilid orthologs

Dpse\GA10384

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000017140
Anopheles gambiae (Ensembl)
ENSBTAP00000018993
Bos taurus (Ensembl)
ENSCAFP00000019849
Canis familiaris (Ensembl)
ENSCINP00000002575
Ciona intestinalis (Ensembl)
NEWSINFRUP00000144031
Takifugu rubripes (Ensembl)
ENSGALP00000002349
Gallus gallus (Ensembl)
ENSGALP00000015277
Gallus gallus (Ensembl)
ENSP00000263168
Homo sapiens (Ensembl)
ENSMMUP00000008923
Macaca mulatta (Ensembl)
ENSMODP00000019731
Monodelphis domestica (Ensembl)
ENSPTRP00000040123
Pan troglodytes (Ensembl)
ENSRNOP00000037217
Rattus norvegicus (Ensembl)
GSTENP00029823001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000047045
Xenopus tropicalis (Ensembl)
Q8H358
Oryza sativa (Gramene)
AT3G05520.1
Arabidopsis thaliana (Arabidopsis)
WBGene00028193
Caenorhabditis briggsae (Wormbase)
WBGene00000292
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-3478
Danio rerio (Zfin)
ZDB-CDNA-040425-3643
Danio rerio (Zfin)
ZDB-GENE-030131-5237
Danio rerio (Zfin)
DDB0191243
Dictyostelium discoideum (Dictybase)
MGI:106227
Mus musculus (Informatics)
YKL007W
Saccharomyces cerevisiae (Yeastgenome)
SPAC12B10.07
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v18648

w¹¹¹⁸ P{GD7009}v18648

Bloomington

13197

y¹; P{SUPor-P}cpa^KG02261/CyO; ry⁵⁰⁶

Genomic Clones ( 1 )

BACR16B07

cDNA Clones ( 31 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH10050

Other clones