• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\Mlf

General Information
Symbol	Dmel\Mlf	Species	D. melanogaster
Name	Myelodysplasia/myeloid leukemia factor	Annotation symbol	CG8295
Feature type	protein_coding_gene	FlyBase ID	FBgn0034051
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	52D2-52D3	Sequence location	2R:11,823,345..11,827,578 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Myelodysplasia/myeloid leukemia factor is referred to in FlyBase by the symbol Mlf (CG8295, FBgn0034051). It has the cytological map location 52D2-52D3. Its sequence location is 2R:11823345..11827578. Its molecular function is unknown. The biological processes in which it is involved are not known. 15 alleles are reported. The phenotypes of these alleles are annotated with 13 unique terms, many of which group under: peripheral nervous system; adult segment; nervous system; adult mesothoracic segment; thoracic segment; adult; organ system; dorsal thoracic disc; larval thorax; wing cell. It has 4 annotated transcripts and 4 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 52D2-52D3   Limits computationally determined from genome sequence between P{lacW}l(2)k02205k02205&P{PZ}sli05248 and P{lacW}ATPCLk09217
Experimentally Determined Cytological Location
Cytogenetic map Notes References 52D-52D (determined by in situ hybridisation)   (Ohno et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: Mlf+ Iap2+ (Ohno et al., 2000)
Gene Model & Products
Please see the GBrowse view of Dmel\Mlf for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts due to variable use of 5' exons
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mlf-RA FBtr0087292  NM_079029   1658   309 Mlf-RB FBtr0087293  NM_166123   1814   273 Mlf-RC FBtr0087294  NM_166122   1798   374 Mlf-RD FBtr0087295  NM_206125   1804   376
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Mlf-PA   FBpp0086428   34.4   309   6.71     NP_523753   AAF58093 Mlf-PB   FBpp0086429   30.4   273   5.14     NP_725502   AAM70969 Mlf-PC   FBpp0086430   41.4   374   10.12     NP_725501   AAM70968 Mlf-PD   FBpp0089346   41.4   376   7.88     NP_995847   AAS64849
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0034051
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264488   AA391063   AA695052   AA802232   AA940727   AA950937   AA978719   AB043986 BAA96391   AC005556   AE003809 AAF58093   AE003809 AAM70968   AE003809 AAM70969   AE003809 AAS64849   AI107772   AI113621   AI113626   AI260802   AI389024   AI512352   AI515548   AI531980   AI532198   AI541593   AI541599   AI945761   AI946948   AW943705   AY037049 AAK63191   AY118583 AAM49952   BF487582   BF488307   BF493298   BF494696   BF496532   BF499862   BF500993   BF501948   BF502407   BG633847   BI161995   BI161996   BI163799   BI167327   BI168738   BI172420   BI230241   BI230799   BI234755   BI234892   BI243250   BI353931   BI357329   BI362599   BI366642   BI367855   BI368181   BI368692   BI370397   BI372370   BI373224   BI481651   BI483582   BI485766   BI563930   BI563957   BI565321   BI577287   BI577690   BI578390   BI579146   BI583425   BI592735   BI616236   BI622142   BI626872   BI632174   BI632467   BI636464   BI636857   BI636962   BI638691   BI640220   BP554226   BP554227   BP555175   BP555176   BP555992   BP555993   BQ103739   BT001895 AAN71684   CK662041   CO182839   CO286870   CO293130   CO297205   CO297996   CO300914   CO307268   CO307741   CO308015   CO309203   CO319343   CO331700   CZ480414   CZ484976   EC056947   EC066022   EC074816   EC078221   EC079941   EC085381   EC089228   EC198389   EC201460   EC203705   EC208849   EC209015   EC212516   EC214945   EC218051   EC224437   EC233244   EC234352   EC234406   EC238071   EC241468   EC247785   EC250208   EC251022   RE01433
UniProtKB/Swiss-Prot	Q9NKV0
UniProtKB/TrEMBL
Maps to	DS08658 DS00960 (Ohno et al., 2000)
Does NOT map to
Identified with	bs16e11 bs33c12 GM04055 LD32726 (Oliver, 2001.8.16) LD44205 SD18148 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12367
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI512352 AA940727
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0034051 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0034051 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0034051 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for Mlf FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Mlf Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | conditional - heat sensitive, with Scer\GAL4ptc-559.1 MlfScer\UAS.cFa lethal | embryonic stage | rescuable maternal effect MlfΔ5-3 MlfΔC1 lethal, with Scer\GAL4da.G32 MlfScer\UAS.cFa Other Phenotypes Allele wild-type, with Scer\GAL4[-]: MlfEU2490 visible MlfΔ5-3 MlfΔC1 visible, with Scer\GAL4Bx-MS1096 MlfScer\UAS.cFa visible, with Scer\GAL4ptc-559.1 MlfScer\UAS.cFa visible, with Scer\GAL4vg.PM MlfScer\UAS.cFa visible, with Scer\GAL4en-e16E MlfScer\UAS.cFa visible, with Scer\GAL4GMR.PF MlfScer\UAS.cFa visible, with Scer\GAL4ey.PH MlfScer\UAS.cFa eye color defective, with Scer\GAL4GMR.PF MlfScer\UAS.cFa cell death increase, with Scer\GAL4en-e16E MlfScer\UAS.cFa melanotic necrosis, with Scer\GAL4GMR.PF MlfScer\UAS.cFa Phenotype manifest in Allele 2nd posterior cell, with Scer\GAL4ptc-559.1 MlfScer\UAS.cFa anterior crossvein, with Scer\GAL4ptc-559.1 MlfScer\UAS.cFa scutellar bristle, with Scer\GAL4ptc-559.1 MlfScer\UAS.cFa wing margin, with Scer\GAL4vg.PM MlfScer\UAS.cFa wing, with Scer\GAL4Bx-MS1096 MlfScer\UAS.cFa wing | posterior compartment, with Scer\GAL4en-e16E MlfScer\UAS.cFa dorsal mesothoracic disc | posterior compartment, with Scer\GAL4en-e16E MlfScer\UAS.cFa ommatidium, with Scer\GAL4ey.PH MlfScer\UAS.cFa interommatidial bristle, with Scer\GAL4ey.PH MlfScer\UAS.cFa eye, with Scer\GAL4ey.PH MlfScer\UAS.cFa eye, with Scer\GAL4GMR.PF MlfScer\UAS.cFa pigment cell, with Scer\GAL4GMR.PF MlfScer\UAS.cFa ommatidium, with Scer\GAL4GMR.PF MlfScer\UAS.cFa interocellar bristle MlfΔC1 MlfΔ5-3 head bristle MlfΔC1 MlfΔ5-3 wing vein | ectopic MlfΔC1 MlfΔ5-3
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of Mlf Class Mutagen Stocks Known lesion MlfC5 Δ2-3 0 Yes MlfEU2490 0 Yes MlfR2 Δ2-3 0 Yes Mlfe02635 piggyBac transposase 0 -- Mlff02458 piggyBac transposase 1 -- Mlfunspecified 0 -- MlfΔ5-3 Δ2-3 0 Yes MlfΔC1 Δ2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mlf Class Mutagen Stocks Known lesion MlfA.Act5C.T:Avic\GFP-EGFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes MlfA.Act5C.T:Disc\RFP-mRFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes MlfB.Act5C.T:Disc\RFP-mRFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes MlfGD13882 in vitro construct | RNAi in vitro construct | regulatory fusion 0 Yes MlfScer\UAS.cFa in vitro construct | regulatory fusion </