Gene Dmel\CG8291

Help

Close All Open All

General Information

Symbol

Dmel\CG8291

Species

D. melanogaster

Name

CG8291

Annotation symbol

CG8291

Feature type

protein_coding_gene

FlyBase ID

FBgn0034049

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

52D2-52D2

Sequence location

2R:11,810,545..11,819,434 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol CG8291 (FBgn0034049). It has the cytological map location 52D2. Its sequence location is 2R:11810545..11819434. Its molecular function is described as: neurotransmitter transporter activity; neurotransmitter:sodium symporter activity. It is involved in the biological process neurotransmitter transport. 5 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

52D2-52D2

Limits computationally determined from genome sequence between P{lacW}l(2)k02205^k02205&P{PZ}sli⁰⁵²⁴⁸ and P{lacW}ATPCL^k09217

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\CG8291 for information on other features

Comments on Gene Model

EST data support existence of multiple transcripts due to variable use of 5' exons

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG8291-RA

FBtr0087298

NM_137220

4401

1331

CG8291-RB

FBtr0087299

NM_166121

4914

1331

CG8291-RC

FBtr0087300

NM_176181

4614

1331

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG8291-PA

FBpp0086433

146.7

1331

6.75

NP_611064

AAF58096

CG8291-PB

FBpp0086434

146.7

1331

6.75

NP_725499

AAM70967

CG8291-PC

FBpp0086435

146.7

1331

6.75

NP_788361

AAO41388

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0034049

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Sodium:neurotransmitter symporter (IPR000175)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

SD06851

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

12380

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI534231
AA950142
AI534226

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034049

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0034049

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0034049

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of CG8291	Mutagen	Stocks	Known lesion
CG8291^KG07083	P-element activity	1	--
CG8291^e04631	piggyBac transposase	1	--
CG8291^f07025	piggyBac transposase	0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG8291	Class	Mutagen	Stocks	Known lesion
CG8291^GD2018		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
CG8291^t7.4		in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)WMG

Useful duplication

Dp(2;2)SMG45

Disrupted in

Df(2R)Exel7138

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD2018}

characterization construct

P{Iap2^+t7.4Wt}

P{Iap2^7.4C472Y}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}CG8291^e04631

PBac{WH}CG8291^f07025

P{SUPor-P}CG8291^KG07083

insertion of enhancer trap

P{PZ}sli⁰⁵²⁴⁸

P{lacW}l(2)SH1673^SH1673

insertion of mobile activating element

P{EP}EP2577

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

neurotransmitter transporter activity

inferred from sequence or structural similarity with UniProtKB:P23978

(FlyBase, 1992-)

inferred from sequence or structural similarity with FLYBASE:DAT; FB:FBgn0034136

(Romero-Calderon et al., 2007)

neurotransmitter:sodium symporter activity

inferred from electronic annotation with InterPro:IPR000175

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

neurotransmitter transport

inferred from sequence or structural similarity with FLYBASE:DAT; FB:FBgn0034136

(Romero-Calderon et al., 2007)

inferred from electronic annotation with InterPro:IPR000175

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

integral to plasma membrane

inferred from electronic annotation with InterPro:IPR000175

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG8291 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62474

Orthologs

Genome-wide drosophilid orthologs

Dana\GF13338

Dere\GG22323

Dgri\GH20963

Dmoj\GI21300

Dper\GL16261

Dpse\GA20961

Dsec\GM20111

Dsim\GD25588

Dvir\GJ20902

Dwil\GK19541

Dyak\GE14119

Curated drosophilid orthologs

Dpse\GA20961

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000016476
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

Szeged

P{EP}EP2577

P{lacW}l(2)SH1673^SH1673

Harvard

PBac{RB}CG8291^e04631

VDRC

v44375

w¹¹¹⁸; P{GD2018}v44375

v37176

w¹¹¹⁸; P{GD2018}v37176/CyO

Bloomington

12189

P{PZ}sli⁰⁵²⁴⁸ cn¹/CyO; ry⁵⁰⁶

14160

y¹ w^67c23; P{SUPor-P}CG8291^KG07083

Genomic Clones ( 1 )

BACR07M03

cDNA Clones ( 28 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

SD06851

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0034049

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0034049

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Sodium:neurotransmitter symporter (IPR000175)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

62474

DEDB - Drosophila exon database: splicing graphs

•

12380

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0034049

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0034049

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0034049

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0034049

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0034049

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0034049

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000016476
Anopheles gambiae (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0034049

Synonyms & Secondary IDs ( 1 )

Reported As

Symbol Synonym

CG8291

(Huh et al., 2007, Quinones-Coello, 2007, Romero-Calderon et al., 2007)

Name Synonym

CG8291

Secondary FlyBase IDs

References ( 15 )

Research paper

Huh et al., 2007, J. Biol. Chem. 282(3): 2056--2068: The Drosophila inhibitor of apoptosis (IAP) DIAP2 is dispensable for cell survival, required for the innate immune response to gram-negative bacterial infection, and can be negatively regulated by the reaper/hid/grim family of IAP-binding apoptosis inducers. [FBrf0192423]
Romero-Calderon et al., 2007, Dev. Neurobiol. 67(5): 550--569: A screen for neurotransmitter transporters expressed in the visual system of Drosophila melanogaster identifies three novel genes. [FBrf0200993]
Kearney et al., 2004, Dev. Biol. 275(2): 473--492: Gene expression profiling of the developing Drosophila CNS midline cells. [FBrf0180108]

Supplementary material

Quinones-Coello, 2007, Genetics 175(3):: Supplemental Table 1. List of genes associated with protein or enhancer traps. [FBrf0200393]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]
Oh et al., 2003, Genetics 163(1):: Table S1. [FBrf0157097]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Misra, 2000.8.25, nc5 report, second installment.: nc5 report, second installment. [FBrf0131112]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.: Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]