A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\opa1-like

General Information
SymbolDmel\opa1-likeSpeciesD. melanogaster
Nameoptic atrophy 1-likeAnnotation symbolCG8479
Feature typeprotein_coding_geneFlyBase IDFBgn0086250
Created / Updated2007-06-14/2007-06-14
Genomic Location
Chromosome (arm)2RRecombination map
Cytogenetic map50E4-50E5Sequence location2R:10,118,125..10,122,953 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene optic atrophy 1-like is referred to in FlyBase by the symbol opa1-like (CG8479, FBgn0086250). It has the cytological map location 50E4-50E5. Its sequence location is 2R:10118125..10122953. Its molecular function is described as: GTP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; GTPase activity. It is involved in the biological process mitochondrial fusion. 5 alleles are reported. The phenotypes of these alleles are annotated with: Nebenkern; mitochondrion. It has 2 annotated transcripts and 2 annotated polypeptides.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
50E4-50E5  
Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
50E4-50E5
(determined by in situ hybridisation)  
(Spradling et al., 1999)
50E4-50E5
 
(BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\opa1-like for information on other features
detailed view FBtr0087532 FBtr0087533 FBtr0087534 FBtr0087574 FBtr0087573 FBtr0087572 FBtr0087571 FBtr0087570 FBtr0087569 FBpp0086661 FBpp0086663 FBpp0086662 FBpp0086700 FBpp0086699 FBpp0086698 FBpp0086696 FBpp0086697 FBpp0086695 FBti0051333 FBti0050821 FBti0005377 FBti0039803
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
opa1-like-RA
FBtr0087574
 
  3207
  972
opa1-like-RB
FBtr0087573
 
  3090
  933
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
opa1-like-PA  
FBpp0086700  
111.8  
972  
8.99  
 
  AAF58275
opa1-like-PB  
FBpp0086699  
107.2  
933  
9.37  
 
  AAM70993
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Dynamin, GTPase region (IPR001401)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA140817
 
AA140818
 
AA539110
 
AA696547
 
AA698723
 
AA979437
 
AE003815
AAF58275
 
AE003815
AAM70993
 
AI064610
 
AI109226
 
AI135885
 
AI405303
 
AI456744
 
AI513269
 
AI517216
 
AI530754
 
AI530937
 
AI533482
 
AI546139
 
AQ026146
 
AY058366
AAL13595
 
BF490272
 
BG635786
 
BG637154
 
BG640452
 
BI169947
 
BI231477
 
BI234463
 
BI352697
 
BI355371
 
BI361476
 
BI363257
 
BI363605
 
BI364695
 
BI565812
 
BI568913
 
BI568914
 
BI573966
 
BI573967
 
BI610530
 
BI612159
 
BI615977
 
BI617896
 
BI623931
 
BI628515
 
BI633018
 
BI637521
 
BI637527
 
BI639822
 
BI639988
 
BI640120
 
BP544171
 
BP544172
 
BQ103258
 
CK00488
 
CL610258
 
CO189440
 
CO284234
 
CO284532
 
CO307438
 
CO309136
 
CO314988
 
CO317641
 
CO341119
 
CZ485328
 
CZ485329
 
CZ486170
 
CZ486171
 
EC065518
 
EC080719
 
EC089210
 
EC212816
 
EC214932
 
EC227046
 
EC258240
 
RH01178
 
RH09410
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    (Spradling et al., 1999)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive
        lethal | first instar larval stage
        semi-lethal
        Phenotype manifest in
        Allele
        Nebenkern | germ-line clone
        mitochondrion | germ-line clone
        hide Classical Alleles ( 4 )
        For All Classical Alleles Show

        Allele of opa1-likeClassMutagenStocksKnown lesion
        opa1-likeEY098631 --
        opa1-likef027791 --
        opa1-likef035941 --
        opa1-likes34751 --
        hide Alleles Carried on Transgenic Constructs ( 1 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of opa1-likeClassMutagenStocksKnown lesion
        opa1-likeGD139360 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Not disrupted in
        (Spradling et al., 1999, BDGP Project Members, 1994-1999)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        UAS construct
        P{GD13936}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        miscellaneous insertions
        PBac{WH}opa1-likef02779
        NA
        PBac{WH}opa1-likef03594
        NA
        insertion of enhancer trap
        P{lacW}opa1-likes3475
        No
        insertion of mobile activating element
        P{EPgy2}CG8479EY09863
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        400bp of dsRNA generated from primer-directed transcription from genomic DNA, corresponding to the gene opa1-like, when transfected into S2 cells results in a majority of cells exhibiting a highly fragmented mitochondrial network.
        (McQuibban et al., 2006)
        hide Gene Ontology: Function, Process & Cellular Component ( 4 )
        hide Molecular Function
        CV term
        Evidence
        References
        GTP binding
        inferred from sequence or structural similarity with EMBL:AB012720; protein_id:BAA32279
        (FlyBase, 1992-)
        GTPase activity
        inferred from electronic annotation with InterPro:IPR001401
        (FlyBase Curators et al., 2004-)
        hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
        inferred from electronic annotation
        (Roth and Foulger, 2002.4.24)
        hide Biological Process
        CV term
        Evidence
        References
        mitochondrial fusion
        inferred from mutant phenotype
        (McQuibban et al., 2006)
        hide Cellular Component
        CV term
        Evidence
        References
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        opa1-like allele
        Gene
        References
        hide External Data
        Linkouts
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents
        hide Stocks Listed in FlyBase ( 4 )
        Harvard
        -
        PBac{WH}opa1-likef02779
        -
        PBac{WH}opa1-likef03594
        Bloomington
        12188
        w1118; P{lacW}opa1-likes3475/CyO
        20054
        y1 w67c23; P{EPgy2}opa1-likeEY09863/CyO
        hide Genomic Clones ( 2 )
        hide cDNA Clones ( 52 )
        Please Note
        This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
        cDNA Clones, Fully Sequenced
        BDGP DGC clones
        Other clones
        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        hide RNAi & Array Information
        Affy Oligo
        Linkouts
        hide Antibody Information
        hide Other Information
        hide Discoverer
        hide Etymology
        hide Identification
        hide Position Effect Variegation Data
        hide Relationship to Other Genes
        Source for database identity of
        Source for identity of: opa1-like CG8479
        (McQuibban et al., 2006)
        Source for database merge of
        Source for merge of: opa1-like l(2)s3475
        (McQuibban et al., 2006)
        Additional comments
        One of the introns of opa1-like has the sequence and structural characteristics of a "mirtron"- mirtrons are encoded as an intron of another gene which accumulate as a lariats after splicing and require debranching enzyme for conversion into a functional miRNA. The mirtron encoded by the opa1-like intron is mir-1016.
        (Ruby et al., 2007)
        hide Comments About Role
        hide Comments About Molecular Function
        hide Other Comments
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        hide External Crossreferences & Linkouts
        Sequence Crossreferences
        DDBJ / EMBL / GenBank
        •
        UniProtKB/TrEMBL
        •
        Other Crossreferences
        InterPro domains - A database of protein families, domains, and functional sites
        •
        Dynamin, GTPase region (IPR001401)
        Linkouts
        hide Synonyms & Secondary IDs ( 9 )
        Reported As
        Symbol Synonym
        CG8479
        (Ruby et al., 2007, Bakal et al., 2007)
        l(2)s3475
         
        OPA1
        (Uemura et al., 2007)
        opa1-like
         
        Opa1-like
        (McQuibban et al., 2006)
        OPA1-like
        (Urban, 2006)
        Name Synonym
        CG8479
         
        Drosophila optic atrophy 1-like
        (McQuibban et al., 2006)
        lethal (2) s3475
         
        optic atrophy 1-like
         
        Secondary FlyBase IDs
        • FBgn0033914
        • FBgn0010687
        hide References ( 18 )
        Research paper
        McQuibban et al., 2006, Curr. Biol. 16(10): 982--989
        Normal mitochondrial dynamics requires rhomboid-7 and affects Drosophila lifespan and neuronal function. [FBrf0195303]
        Spradling et al., 1999, Genetics 153(1): 135--177
        The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
        Supplementary material
        Bakal et al., 2007, Science 316(5832):
        Supporting online material. [FBrf0204776]
        Dietzl et al., 2007, Nature 448(7150):
        Supplementary Table 1. [FBrf0202579]
        Ruby et al., 2007, Nature 448(7149):
        Supplementary information. [FBrf0202573]
        Giot et al., 2003, Science 302(5651):
        Tables S1 to S7. [FBrf0166452]
        Kiger et al., 2003, J. Biol. 2(4):
        Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
        Review
        Urban, 2006, Genes Dev. 20(22): 3054--3068
        Rhomboid proteins: conserved membrane proteases with divergent biological functions. [FBrf0193506]
        Personal communication to FlyBase
        FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.
        Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
        Roth and Foulger, 2002.4.24, Predicted GO terms.
        Predicted GO terms. [FBrf0146674]
        Robertson, 2001.7.7, Third set of annotation suggestions from bee ESTs.
        Third set of annotation suggestions from bee ESTs. [FBrf0138600]
        Abstract
        Uemura et al., 2007, Europ. Dros. Res. Conf. 20: TD007
        THE IMPORTANCE OF CONTROLLING MITOCHONDRIAL FUSION IN GENERATION AND MAINTENANCE OF DENDRITIC BRANCHES. [FBrf0199938]
        FlyBase analysis
        FlyBase, 1992-, FlyBase curation
        FlyBase curation. [FBrf0105495]
        Computer file
        Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.
        Genomic mapping of Exelixis insertion collection. [FBrf0184340]
        Gene Disruption Project members, 2001-, [title not yet available]
        [title not yet available] [FBrf0132177]
        BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
        Berkeley Drosophila Genome Project. [FBrf0125078]
        BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
        Berkeley Drosophila Genome Project. [FBrf0067338]
        Automatic genome annotation
        FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]
        [Annotations performed at the Celera Jamboreee.] [FBrf0126705]