A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\achi

General Information
SymbolDmel\achiSpeciesD. melanogaster
NameachintyaAnnotation symbolCG8819
Feature typeprotein_coding_geneFlyBase IDFBgn0033749
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2RRecombination map2-65.5 +/- 3.3
Cytogenetic map49A10-49A10Sequence location2R:8,399,035..8,402,556 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene achintya is referred to in FlyBase by the symbol achi (CG8819, FBgn0033749). It has the cytological map location 49A10. Its sequence location is 2R:8399035..8402556. Its molecular function is described as: transcription corepressor activity; transcription factor activity; sequence-specific DNA binding. It is involved in the biological processes: spermatogenesis; regulation of transcription; regulation of transcription, DNA-dependent. 15 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
49A10-49A10  
Limits computationally determined from genome sequence between P{lacW}fdlk17040 and P{EP}CG8569EP1182  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
2-65.5 +/- 3.3
(White-Cooper, 1999.3.3)
Left of (cM)
(White-Cooper, 1999.3.3)
Right of (cM)
(White-Cooper, 1999.3.3)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
Overall orientation not stated: achi+ vis+
(Ayyar and White, 2001)
hide Gene Model & Products
Please see the GBrowse view of Dmel\achi for information on other features
detailed view FBtr0087944 FBtr0087945 FBtr0087943 FBtr0087942 FBtr0087939 FBpp0087055 FBpp0087056 FBpp0087054 FBpp0087053 FBpp0087050 FBti0072752 FBti0038691 FBti0047497 FBti0010854 FBti0047982 FBti0033554
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
achi-RA
FBtr0087943
 NM_165911
  2115
  426
achi-RC
FBtr0087942
 NM_165912
  2502
  555
Additional Transcript Data & Comments
Reported size (kB)
1.6, 1.2 (northern blot)
(Ayyar et al., 2003)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
achi-PA  
FBpp0087054  
47.7  
426  
4.45  
  NP_725182
  AAF58497
achi-PC  
FBpp0087053  
61.5  
555  
4.85  
  NP_725183
  AAM68695
Additional Polypeptide Data & Comments
Reported size (kD)
80, 60 (kD)
(Wang and Mann, 2003)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Homeobox (IPR001356)
Homeodomain-like (IPR009057)
Homeodomain-related (IPR012287)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA694812
 
AA941119
 
AE003822
AAF58497
 
AE003822
AAM68695
 
AI258795
 
AI516196
 
AI946346
 
AQ072908
 
BE976409
 
BG638437
 
BG641012
 
BI235076
 
BI366278
 
BI371574
 
BI371642
 
BT003510
AAO39514
 
CL525876
 
CO189003
 
CO270041
 
CO275866
 
CO285616
 
CO323783
 
CO329264
 
CO342424
 
CZ479917
 
CZ479918
 
EC069349
 
EC071195
 
EC197291
 
EC197467
 
EC212060
 
EC216160
 
EC245981
 
EC256951
 
LD25085
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Oliver, 2001.8.16)
    (Oliver, 2001.8.16, Wang and Mann, 2003)
    (Wang and Mann, 2003)
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    achi[Z3922]
    2R:8,400,935..8,400,935
    comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The line is found to be a combination of two alleles, achi[Z3922] and vis[Z3922].
    evidence=experimental
    na_change=C8400935T
    pr_change=Q230|achi-PC,Q230|achi-PA
    reported_pr_change=Q230@
    (Ayyar et al., 2003)
    rescue fragment
    achi[+t4.8]
    2R:8,398,518..8,403,408
    comment=Rescues the male sterility of homozygous Df(2R)pingpong animals; position of rescue fragment on reference sequence inferred by FlyBase curator.
    evidence=experimental
    (Wang and Mann, 2003)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     adult stage
     whole organism
    (Ayyar et al., 2003)
     adult stage
     testis | restricted
    (Ayyar et al., 2003)
     embryonic stage-adult stage
     whole organism
    (Ayyar et al., 2003)
     adult stage
     testes
    (Ayyar et al., 2003)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       adult stage
       whole organism
      (Wang and Mann, 2003)
       embryonic stage
       whole organism
      (Wang and Mann, 2003)
       larval stage
       dorsal mesothoracic disc
      (Wang and Mann, 2003)
       adult stage
       egg chamber
      (Wang and Mann, 2003)
       adult stage
       testis
      (Wang and Mann, 2003)
       adult stage
       spermatid
      (Wang and Mann, 2003)
       adult stage
       spermatocyte
      (Wang and Mann, 2003)
       adult stage
       spermatocyte
      (Wang and Mann, 2003)
       adult stage
       testis
      (Wang and Mann, 2003)
       adult stage
       ovary
      (Wang and Mann, 2003)
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0087944 FBtr0087945 FBtr0087943 FBtr0087942 FBtr0087939
        AA941119
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for achi
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        viable, with Scer\GAL4[-]:
        Sterility
        Allele
        male sterile | partially, with Scer\GAL4[-]:
        fertile
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 10 )
        For All Classical Alleles Show

        Allele of achiClassMutagenStocksKnown lesion
        achi10 Yes
        achi20 Yes
        achi30 Yes
        achi40 Yes
        achiDG163091 --
        achiEP21072 Yes
        achiEY030841 --
        achiZ39220 Yes
        achie020901 --
        achipingpong
          		3 Yes
          hide Alleles Carried on Transgenic Constructs ( 5 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of achiClassMutagenStocksKnown lesion
          achi+t4.81 Yes
          achiGD13470 Yes
          achiGD175393 Yes
          achiL.αTub84B1 Yes
          achiS2.αTub84B0 Yes
          hide Aneuploid Aberrations
          Useful duplication
           
          Disrupted in
          (Ayyar et al., 2003)
          (Ayyar et al., 2003)
          (Ayyar et al., 2003)
          (Ayyar et al., 2003)
          (Wang and Mann, 2003)
          hide Transgenic Constructs & Insertions
          Transgenic Constructs
          Type of construct
          Name
          Expression data
          UAS construct
          P{GD1347}
          NA
          P{GD17539}
          NA
          P{GD4395}
          NA
          characterization construct
          P{achi}
          NA
          P{tub-achi.L}
          NA
          P{tub-achi.S2}
          NA
          Insertions
          Type of insertions
          Name
          Expression data
          miscellaneous insertions
          PBac{RB}achie02090
          NA
          P{wHy}achiDG16309
          NA
          insertion of mobile activating element
          P{EPgy2}achiEY03084
          NA
          P{EP}achiEP2107
          NA
          hide Related Comments
          Please look at the allele reports for the complete phenotype data
          hide Gene Ontology: Function, Process & Cellular Component ( 7 )
          hide Molecular Function
          CV term
          Evidence
          References
          sequence-specific DNA binding
          inferred from electronic annotation with InterPro:IPR001356
          (FlyBase Curators et al., 2004-)
          transcription corepressor activity
          inferred from sequence or structural similarity
          (Ayyar and White, 2001)
          transcription factor activity
          inferred from sequence or structural similarity with NCBI_NP:777480
          (FlyBase, 1992-)
          hide Biological Process
          CV term
          Evidence
          References
          regulation of transcription
          inferred from sequence or structural similarity with NCBI_NP:777480
          (FlyBase, 1992-)
          regulation of transcription, DNA-dependent
          inferred from electronic annotation with InterPro:IPR001356
          (FlyBase Curators et al., 2004-)
          spermatogenesis
          inferred from expression pattern AND inferred from mutant phenotype
          (Ayyar et al., 2003)
          inferred from expression pattern AND inferred from mutant phenotype AND inferred from physical interaction with FLYBASE:aly; FB:FBgn0004372 AND inferred from physical interaction with FLYBASE:comr; FB:FBgn0034667
          (Wang and Mann, 2003)
          hide Cellular Component
          CV term
          Evidence
          References
          nucleus
          inferred from sequence or structural similarity
          (Ayyar and White, 2001)
          inferred from sequence or structural similarity with NCBI_NP:777480
          (FlyBase, 1992-)
          hide Sequence Ontology: Class of Gene
          protein_coding_gene
           
          nuclear_gene
           
          hide Interactions & Pathways
          hide Summary of Genetic Interactions
          Interacts with
          Please look at the allele data for full details of the genetic interactions
          achi allele
          Gene
          References
          achi1
          vis
          (Ayyar et al., 2003)
          achiZ3922
          vis
          (Ayyar et al., 2003)
          hide External Data
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          Drosophila PIMRider - The Drosophila Protein Interaction map
          •
          hide Orthologs
          Genome-wide drosophilid orthologs
          Curated drosophilid orthologs
           
          Linkouts
          InParanoid orthologs - Eukaryotic orthologs
          •
          hide Functional Complementation between Species
          hide Inter-Species Misexpression Data
          Produces phenotype in
          Produces NO phenotype in
          hide Stocks & Reagents
          hide Stocks Listed in FlyBase ( 12 )
          Szeged
          -
          P{EP}achiEP2107
          Harvard
          -
          PBac{RB}achie02090
          VDRC
          v49635
          w1118; P{GD17539}v49635
          v49637
          w1118; P{GD17539}v49637
          v52632
          w1118; P{GD17539}v52632/TM3
          Bloomington
          17025
          w1118; P{EP}achiEP2107
          8512
          w*; P{tub-achi.L}3/TM6B, Tb1
          8510
          w*; Df(2R)pingpong, achipingpong/CyO
          21530
          y1 w67c23; P{wHy}achiDG16309
          17315
          y1 w67c23; P{EPgy2}achiEY03084
          8509
          w*; Df(2R)pingpong, achipingpong/CyO; P{vis}3/MKRS
          8511
          y1 w*/Dp(1;Y)y+; Df(2R)pingpong, achipingpong/CyO; P{achi}3
          hide Genomic Clones ( 1 )
          hide cDNA Clones ( 18 )
          Please Note
          This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
          cDNA Clones, Fully Sequenced
          BDGP DGC clones
          Other clones
          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          hide RNAi & Array Information
          Affy Oligo
          Linkouts
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          hide Antibody Information
          polyclonal
          (Wang and Mann, 2003)
          hide Other Information
          hide Discoverer
          hide Etymology
          Etymology: "achintya" is a Sanskrit word meaning "inconceivable" or "beyond thought", reflecting the initial complexity of the molecular anlysis of the locus.
          (Ayyar, 2001.3.5)
          Etymology: 'achintya' is a Sanskrit word meaning 'that which is beyond thought and contemplation'.
          (Ayyar et al., 2003)
          hide Identification
          hide Position Effect Variegation Data
          hide Relationship to Other Genes
          Source for database identity of
          Source for identity of: achintya CG8819
          (Ayyar, 2001.3.5)
          Source for database merge of
          Additional comments
          The zaa line is found to be a combination of two alleles visZ3922 and visZ3922.
          (Ayyar et al., 2003)
          hide Comments About Role
          achi and vis are essential for developmentally regulated transcription during spermatogenesis.
          (Ayyar et al., 2003)
          achi is required during spermatogenesis.
          (Wang and Mann, 2003)
          hide Comments About Molecular Function
          achi, vis, achi and comr proteins may exist in a complex.
          (Wang and Mann, 2003)
          hide Other Comments
          aly-like meiotic arrest gene.
          (White-Cooper and Fuller, 1999)
          hide External Crossreferences & Linkouts
          Sequence Crossreferences
          RefSeq (Transcripts)
          •
          RefSeq (Proteins)
          •
          DDBJ / EMBL / GenBank
          •
          UniProtKB/TrEMBL
          •
          Other Crossreferences
          InterPro domains - A database of protein families, domains, and functional sites
          •
          Homeobox (IPR001356)
          Homeodomain-like (IPR009057)
          Homeodomain-related (IPR012287)
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          DEDB - Drosophila exon database: splicing graphs
          •
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          Drosophila PIMRider - The Drosophila Protein Interaction map
          •
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          •
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          •
          FlyExpress - Embryonic expression images (BDGP data)
          •
          FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
          •
          GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          InParanoid orthologs - Eukaryotic orthologs
          •